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IRF6 — NFKBIA

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Liu et al., Mol Pharmacol 1999 (Shock, Septic) : Pretreatment of rats with pyrrolidine dithiocarbamate ( 50, 100, and 200 mg/kg, i.p. ), an inhibitor of NF-kappaB activation, prevented LPS induced I-kappaBalpha degradation and the resultant NF-kappaB activation and inhibited, in a dose related manner, the LPS induced CINC and ICAM-1 mRNA and protein expression
Han et al., Eur J Immunol 1999 : The LPS induced degradation of IkappaBalpha was prevented by pretreatment with BN 50739, suggesting that PAF is a critical intermediate in the LPS triggered degradation of IkappaBalpha protein ... DEX prevented the LPS induced IkappaBalpha degradation, which was also reversed by exogenous PAF
Kramer et al., J Surg Res 1999 (Hemorrhage...) : LPS stimulation of sham Mphi increased NF-kappaB activation with corresponding loss of its inhibitor IkappaB-alpha
Lauzurica et al., Eur J Immunol 1999 (Disease Models, Animal...) : The in vivo effect of PDTC on NF-kappaB activation in liver tissues involved the inhibition of both LPS induced I kappaB-alpha degradation and the translocation of the p50 and p65 NF-kappaB subunits to the nucleus
Laflamme et al., J Neurochem 1999 (Inflammation) : Systemic LPS , IL-1beta, and TNF-alpha caused a rapid and transient transcriptional activation of IkappaB alpha along the blood vessels of the entire brain ; the signal was very intense 30-60 min after the i.v. injections and returned to undetectable levels from 2 to 12 h depending on the challenge
Simpson et al., J Neurochem 1999 : Simultaneously, the cytoplasmic expression of phosphorylated inhibitory protein IkappaB alpha was dramatically increased by SNAP ( 200 microM ), LPS ( 10 microg/ml ), and kainate ( 50 microM ) treatment
Mustafa et al., Am J Physiol 1999 : Treatment of KC with calcium channel antagonists prior to the addition of LPS decreased nuclear levels of the p65 subunit of nuclear factor-kappaB and prevented the LPS dependent degradation of the inhibitory protein IkappaBalpha
Raychaudhuri et al., Am J Respir Cell Mol Biol 1999 (Asthma...) : NO prevented an LPS mediated decrease in the NF-kappaB inhibitory protein IkappaB-alpha
Mustafa et al., Hepatology 1999 (Endotoxemia) : There was concomitant inhibition of LPS dependent degradation of the inhibitory protein IkappaBalpha and increase in intracellular Ca ( 2+ ) in KC treated with BN 50739 or WEB 2170
Mandrekar et al., Int Immunol 1999 : Alcohol did not prevent LPS induced IkappaBalpha degradation but decreased the levels of phospho-specific IkappaBalpha ( Ser32 )
Medvedev et al., J Immunol 2000 (MAP Kinase Signaling System) : Pretreatment of mouse macrophages with LPS inhibited phosphorylation of the extracellular signal regulated kinases, c-Jun NH2-terminal kinases, and p38 kinase ; degradation of I-kappaBalpha ( inhibitory protein that dissociates from NF-kappaB ) and I-kappaBbeta ; and activation of the transcription factors NF-kappaB and AP-1 in response to subsequent LPS stimulation
Williams et al., Shock 2000 (Intestinal Perforation...) : Pre- or post-treatment with glucan phosphate decreased ( P < 0.05 ) tissue phospho-IkappaBalpha levels in CLP mice. Phospho-IkappaBalpha in the glucan-CLP group were not significantly different from the unoperated controls. To investigate mechanisms we examined IKKbeta kinase activity, IkappaBalpha phosphorylation and degradation, and NFkappaB activity in a murine macrophage cell line, J774a.1, treated with LPS ( 1 microg/mL ) and/or glucan phosphate ( 1 microg/mL ) for up to 120 min. The glucan ligand blunted LPS induced IKKbeta kinase activity, phosphorylation and degradation of IkappaBalpha , and NFkappaB nuclear binding activity. The data indicate that one mechanism by which ( 1 -- > 3 ) -beta-D-glucan may alter the response to endotoxin or polymicrobial sepsis involves modulation of IKK3 kinase activity with subsequent decreases in IkappaBalpha phosphorylation and NFkappaB activation
Xia et al., Biochem Pharmacol 2000 : However, both the LPS induced degradation of the cytoplasmic NF-kappaB inhibitor IkappaBalpha and the nuclear translocation of the NF-kappaB p50, p65, and c-Rel peptides were unaffected by treatment of the cells with the nucleoside
Daun et al., Am J Physiol Regul Integr Comp Physiol 2000 : Physiological doses of hydrocortisone ( 50-200 ng/ml ) diminished both the LPS stimulated decrease in cytosolic IkappaBalpha levels and the subsequent increase in nuclear NF-kappaB DNA binding ... In the absence of hydrocortisone, MIF had no effect on LPS induced decreases in IkappaBalpha
Kim et al., Liver 2000 : Pretreatment of rats with DDB prevented LPS induced hepatic I-kappaBalpha degradation and the resultant NF-kappaB activation, and inhibited the LPS induced plasma TNF-alpha protein and hepatic TNF-alpha mRNA expression in a dose dependent manner ... LPS induced I-kappaBalpha degradation and TNF-alpha production were also inhibited by DDB in RAW264.7 cells, which was consistent with the results in rats
Choi et al., Int J Immunopharmacol 2000 : LPS reduced I-kappaBalpha level in RAW264.7 cells by 77 % ... ACF attenuated LPS induced decrease in I-kappaBalpha protein in a concentration dependent manner
Farmer et al., Am J Physiol Lung Cell Mol Physiol 2000 : Although the initial IkappaB-alpha degradation induced by LPS was minimally affected by beta-agonists, the latter induced a marked rebound of the cytosolic IkappaB-alpha levels at later time points ( > 1 h ), accompanied by an increased IkappaB-alpha cytoplasmic half-life
Shanley et al., Shock 2000 (Inflammation) : Heat shock, 1 h before treatment with LPS, completely inhibited LPS mediated degradation of I-kappaBalpha ... Western-blot analyses using a phosphorylated I-kappaBalpha-specific antibody demonstrated that heat shock inhibited LPS mediated phosphorylation of I-kappaBalpha ... We conclude that the mechanism by which heat shock inhibits LPS mediated degradation of I-kappaBalpha involves specific inhibition of I-kappaBalpha phosphorylation and subsequent I-kappaBalpha ubiquitination
Xagorari et al., J Pharmacol Exp Ther 2001 (Inflammation) : Pretreatment of cells with luteolin abolished the effects of LPS on IkappaB-alpha
Cruz et al., Nitric Oxide 2001 : LPS induction of I kappa B-alpha degradation and iNOS expression in a skin dendritic cell line is prevented by the janus kinase 2 inhibitor, Tyrphostin b42 ... In addition, LPS induces the activation of NF-kappa B, and tyrphostin B42 prevents the degradation of the cytosolic factor I kappa B-alpha and blocks the translocation of the NF-kappa B p65 protein subunit into the nucleus
Nomura et al., Life Sci 2001 : Experiments using stable clones of rat C6 glioma cells transfected with dominant negative IkappaB alpha ( serines 32 and 36 replaced by alanine ) suggest that NF-kappaB activation ( phosphorylation of IkappaB alpha ) is involved in LPS/IFNgamma- or IL-1beta/IFNgamma induced iNOS expression
Lancaster et al., Inflammation 2001 (Endotoxemia...) : Treatment with Cl-1 prevented degradation of IkappaB-alpha and activation of NF-kappaB in the liver in response to LPS ; however, Cl-1 treatment had no detected effect on NF-kappaB activation in lung tissue
Koo et al., Planta Med 2001 : CTN also significantly inhibited LPS induced DNA binding activity of NF-kappa B as well as the LPS induced degradation of I kappa B-alpha and -beta ... Furthermore, CTN inhibited LPS induced phosphorylation of I kappa B-alpha
Ferlito et al., Shock 2001 (MAP Kinase Signaling System) : Pretreatment with pertussis toxin, which inactivates inhibitor G protein ( G alpha i ) function, significantly inhibited LPS induced p38 phosphorylation, but not LPS induced IkappaB alpha degradation ... LPS desensitized cells exhibited augmented IkappaB alpha content and were refractory to LPS induced IkappaB alpha degradation and p38 phosphorylation
Guyton et al., J Leukoc Biol 2001 : BRL inhibited LPS induced NO, but augmented LPS induced TNF-alpha and TxB2. 15-PGJ2 also inhibited degradation of LPS induced IkappaB alpha and phosphoactivation of ERK 1/2, but BRL had no significant effect on either protein
Haddad et al., Br J Pharmacol 2001 : Pre-treatment with Y-40138 ameliorated LPS induced degradation of I kappa B-alpha in the cytosolic compartment and retarded its phosphorylation, suggesting the involvement of an upstream kinase
Dokka et al., Am J Physiol Lung Cell Mol Physiol 2001 : Addition of IL-10 inhibited both IkappaB-alpha degradation and generation of.OH radicals in response to LPS stimulation
Saha et al., J Leukoc Biol 2001 : In addition, we have shown that following MPL pretreatment, LPS stimulation does not cause the loss of cytoplasmic IkappaBalpha , which occurs when human monocytes are cultured with LPS
Ferlito et al., J Leukoc Biol 2001 (Leukemia, Monocytic, Acute...) : In naive cells, LPS , TNF-alpha, and IL-1beta induced IkappaBalpha degradation, kinase phosphorylation, and NF-kappaB DNA binding
Haddad et al., Am J Respir Cell Mol Biol 2002 : Amiloride and HMA suppressed the phosphorylation of IkappaB-alpha mediated by LPS , thereby allowing its cytosolic accumulation
Li et al., Cytokine 2001 : LPS did not induce degradation of the inhibitory protein I-kappa B alpha in the shocked cells, which might be a potential mechanism to block NF-kappa B activation
Altavilla et al., Cardiovasc Res 2002 (Shock, Septic) : Moreover we studied in LPS stimulated ( 50 microg ml(-1) ) peritoneal macrophages ( Mphi ), NF-kappaB activation, cytoplasmic IkappaB-alpha degradation, the message for TNF-alpha, and TNF-alpha and MAL levels ... In addition, in vitro LPS stimulation ( 50 microg ml(-1) ) significantly induced NF-kappaB activation and cytoplasmic IkappaBalpha degradation in Mphi, enhanced TNF-alpha mRNA levels and increased Mphi TNF-alpha and MAL
Ward et al., J Immunol 2002 : Furthermore, Delta ( 12 ) PGJ ( 2 ) and 15dPGJ ( 2 ) inhibited LPS induced I kappa B alpha degradation and subsequent inhibition of neutrophil apoptosis, suggesting that apoptosis is mediated via PPAR-gamma independent inhibition of NF-kappa B activation
Lo et al., Carcinogenesis 2002 : These activities were referred to down-regulation of inhibitor kappaB (IkappaB) kinase ( IKK ) activity by carnosol ( 5 microM ), thus inhibited LPS induced phosphorylation as well as degradation of IkappaBalpha
De Plaen et al., Immunology 2002 : These observations suggest that LPS causes slow degradation and synthesis of IkappaBalpha and IkappaBbeta and therefore activates NF-kappaBeta via at least two mechanisms : initially, through an IkappaB independent mechanism, and later, via an increased turnover of the inhibitor IkappaB
Yu et al., Ann Clin Lab Sci 2002 : LPS stimulation of rat PBMC increased TNFalpha production and NFkappaB activation, with corresponding loss of IkappaBalpha
Zhou et al., J Biol Chem 2002 : Western blot analyses were performed showing that RON activation inhibits LPS induced I kappa B alpha degradation
Aksoy et al., Eur J Immunol 2002 : Furthermore, we found that PKC inhibition impaired LPS induced I kappa B-alpha degradation and subsequent nuclear factor (NF)-kappa B activation in DC
Ishii et al., Biochim Biophys Acta 2003 : Western blot analysis showed that LPS reduced the IkappaB-alpha level in macrophages, while 10 microM isomallotochromanol and 10 microM isomallotochromene attenuated the LPS induced decrease in IkappaB-alpha protein
Haller et al., J Biol Chem 2003 (Bacteroides Infections) : The inhibitory effect of TGF-beta1 is mediated independently of B. vulgatus/LPS induced IkappaBalpha , Akt, and RelA phosphorylation as well as NF-kappaB DNA binding activity
Spek et al., J Clin Immunol 2003 : LPS induced IkappaBalpha production, whereas p50 was unresponsive and p65 and p49/p100 remained undetectable ... LPS induced IkappaBalpha expression was delayed ( 1 hr ) and reduced by IC14
Yang et al., J Immunol 2003 : Activation of the I kappa B kinase (IKK) complex by LPS induces phosphorylation and degradation of I kappa B alpha , leading to the nuclear translocation of NF-kappa B
Huang et al., J Biomed Sci 2003 : We found that LPS induced I kappa B kinase and nuclear factor-kappa B (NF-kappa B) activation, as well as IFN-gamma induced signal transducer and activator of transcription 1 ( STAT1 ) phosphorylation, were reduced by lovastatin
Ma et al., Mol Pharmacol 2003 : Analyses of the NF-kappaB activation pathway revealed that the antioxidants do not inhibit LPS induced activation of the IkappaB kinase activity, degradation of IkappaBalpha, or translocation of activated NF-kappaB into the nucleus, but they do block the formation of NF-kappaB/DNA binding complexes
Andreakos et al., Blood 2004 : We found that MyD88 and Mal/TIRAP are essential for LPS induced I kappa B alpha phosphorylation, NF-kappa B activation, and interleukin 6 (IL-6) or IL-8 production in fibroblasts and endothelial cells in a pathway that also requires IKK2
Cao et al., Acta Pharmacol Sin 2003 (Respiratory Distress Syndrome, Adult) : Immunoblotting of AM cytoplasmic extract showed that LPS stimulation of AM resulted in the low expression of IkappaB-alpha protein, which was not observed in the presence of SNP and methylprednisolone
Aga et al., Am J Physiol Cell Physiol 2004 (MAP Kinase Signaling System) : The observation that U0126 and BzATP exhibit overlapping actions with respect to LPS induced changes in I kappa B alpha levels is supported by the finding that Ras activation, which is upstream of MEK/ERK activation, is reduced upon macrophage cotreatment with BzATP and LPS compared with the effects of BzATP treatment alone
Hwang et al., Planta Med 2003 : However, an electrophoretic mobility shift assay ( EMSA ) demonstrated that sauchinone did not suppress the DNA binding activity of NF-kappaB or the degradation of IkappaB-alpha induced by LPS
Zhao et al., J Am Coll Nutr 2004 : LPS induced NF-kappaB activation, translocation of p65 subunit to the nucleus, phosphorylation and degradation of IkappaB-alpha were partially prevented by EPA
Barhoumi et al., Brain Res Mol Brain Res 2004 (Manganese Poisoning) : Phosphorylation of IkappaBalpha was potentiated by Mn and LPS and was not blocked by U0126, a selective inhibitor of ERK1/2 ... Blocking mitochondrial ROS also attenuated the enhancing effect of Mn on LPS induced phosphorylation of IkappaBalpha and expression of NOS2, suggesting a link between Mn-induced mitochondrial dysfunction and activation of NF-kappaB ... Overexpression of a dominant negative mutant of the NF-kappaB interacting kinase (Nik) prevented enhancement of LPS induced phosphorylation of IkappaBalpha by Mn
Lee et al., Int J Mol Med 2004 (Pneumonia) : LPS induced expression of I-kappaBalpha , which is expressed by LPS induced activation of NF-kappaB, is inhibited by AEPR as well
Jeong et al., Pharm Res 2004 : ITCs, CUR, ( - ) -epigallocatechin-3-gallate ( EGCG ), and RES reduced LPS induced IkappaBalpha phosphorylation
Yang et al., Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi 2003 : LPS can induce IKK-beta and NF-kappaB activation, IkappaB-alpha degradation, accelerate TNF-alpha release, but PMB can counteract those effects induced by LPS stimulation
Hattori et al., Cardiovasc Res 2004 : LPS stimulates IkappaB-alpha phosphorylation by inducing IkappaB kinase (IKK) activity, and NO, but not ONOO-, inhibits LPS induced IkappaB-alpha phosphorylation and IKK activity
Olsen et al., Int J Cancer 2004 (Carcinoma, Non-Small-Cell Lung...) : Moreover, CHS 828 has also been shown to inhibit the LPS induced degradation of the I kappa B alpha and I kappa B beta in THP-1 cells, leading us to identify the I kappa B kinase complex as a molecular target of CHS 828
Ruiz et al., J Biol Chem 2004 : This inhibitory effect of 15d-PGJ ( 2 ) was mediated independently of LPS induced IkappaBalpha phosphorylation/degradation and RelA nuclear translocation as well as RelA DNA binding activity
Migita et al., FEBS Lett 2004 : Stimulation of human hepatocytes with LPS results in rapid degradation of IkappaB-alpha and mitogen activated protein kinase activation
Ichikawa et al., Biol Pharm Bull 2004 : In addition, both EGCG and GCG inhibited LPS induced degradation of IkappaBalpha with concomitant inhibition of nuclear protein binding to NF-kappaB site and synthesis of IRF-1
Yip et al., J Bone Miner Res 2004 (Bone Resorption...) : Furthermore, PAR inhibited LPS induced NF-kappaB activation, p65 translocation, and IkappaB-alpha degradation both in mature osteoclasts and their precursors in a time- and dose dependent manner
Bi et al., Int Immunopharmacol 2005 : Further studies revealed that resveratrol suppressed LPS induced degradation of IkappaBalpha , expression of iNOS and phosphorylation of p38 mitogen activated protein kinases ( MAPKs ) in N9 microglial cells
Syrovets et al., J Immunol 2005 : Instead, both pentacyclic triterpenes inhibited the LPS induced degradation of IkappaBalpha , as well as phosphorylation of p65 at Ser ( 536 ) and its nuclear translocation
Sangrador-Vegas et al., Vet Immunol Immunopathol 2005 : Expression of IkappaBalpha is up-regulated by LPS and TNFalpha treatment, two known activators of NF-kappaB, suggesting the existence of an autoregulatory loop in fish, as is the case for mammals
Fiebich et al., Planta Med 2005 : C and D did not affect the LPS induced activation of p38 MAPK and IkappaBalpha , but they prevented the LPS induced activation of p42/44 MAPK
Yang et al., Biochem Biophys Res Commun 2005 : The two peptides were found to significantly inhibit LPS induced NF-kappaB activation in HEK-293 cells that were transfected with TLR4 cDNA, LPS induced IkappaBalpha ( IkappaB alpha ) phosphorylation and NF-kappaB activation in monocytes, and release of IL-1, IL-6, and TNF-alpha by monocytes
Hebeis et al., Blood 2005 : Likewise, activation of Akt ( a PI3K [ phosphatidylinositol 3-kinase ] target ) and phosphorylation of IkappaBalpha in response to CD180 or LPS required Vav1 and Vav2, while Vav1 deficiency led to defective responses to CD180
Ueta et al., Biochem Biophys Res Commun 2005 : Similarly, polyI : C, but not LPS , induced the gene expression of IkappaBalpha and MAIL, members of the IkappaB family, in HCEC
Doyle et al., J Biol Chem 2005 : Treatment of the murine monocytic cell line Raw264.7 with LFM-A13, a specific Btk inhibitor, blocked LPS induced NFkappaB dependent reporter gene expression but not IkappaB alpha degradation
Kim et al., Biochem Pharmacol 2005 : Furthermore, the compound inhibited LPS mediated nuclear translocation of NF-kappaB p65 and DNA binding activity of NF-kappaB complex, in parallel, but did not influence LPS mediated IkappaBalpha degradation
Shimomura-Shimizu et al., Biochem Biophys Res Commun 2005 : LPS induced nuclear translocation of NF-kappaB, which is necessary for the expression of IFN-beta and iNOS, was inhibited by these chemicals : however, the LPS induced degradation of IkappaB-alpha and IkappaB-beta was inhibited only by alachlor
Kim et al., Immunology 2005 : Moreover, in vitro kinase assays show that luteolin directly inhibits LPS induced IkappaB kinase (IKK) activity in IEC-18 cells
Kim et al., Clin Exp Immunol 2005 (Translocation, Genetic) : SME significantly blocked LPS induced EGFP expression and IkappaBalpha phosphorylation in intestinal explants and primary IECs, respectively
Aoki et al., J Pharmacol Sci 2005 (Glioma) : This crude drug decreased LPS induced IkappaBalpha degradation in a concentration dependent manner without affecting LPS induced IkappaB phosphorylation
Qiao et al., Free radical research 2005 : Moreover, Western blot analysis demonstrated that LPS induced phosphorylation of Ikappa-Balpha was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite mediated damage, and as a potent inhibitor of superoxide and NO synthesis ; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Ikappa-Balpha
Youn et al., J Immunol 2005 : The suppressive effect of resveratrol on LPS induced NF-kappaB activation was abolished in TRIF-deficient mouse embryonic fibroblasts, whereas LPS induced degradation of IkappaBalpha and expression of cyclooxygenase-2 and inducible NO synthase were still inhibited in MyD88-deficient macrophages
Fukuda et al., Invest Ophthalmol Vis Sci 2005 : Combined stimulation with LPS and either sCD14 or LBP also induced the phosphorylation and degradation of IkappaB-alpha and the translocation of NF-kappaB from the cytoplasm to the nucleus of corneal fibroblasts
Uto et al., Biochem Pharmacol 2005 : LPS caused the c-Jun phosphorylation ( a major component of AP-1 ) and IkappaB-alpha degradation ... 6-MITC suppressed LPS induced c-Jun phosphorylation, but did not inhibit IkappaB-alpha degradation
Campa et al., Inflamm Res 2005 (Inflammation) : Thus, poly I induced an increase in nuclear factor kappaB (NF-kappaB) transcriptional activity due to a long-term degradation of inhibitory NF-kappaB (IkappaB) beta while lipopolysaccharide (LPS) induced the degradation of both IkappaBalpha and IkappaBbeta
Kim et al., Biochem Biophys Res Commun 2005 : Rottlerin pretreatment also attenuated LPS induced phosphorylation of IkappaB-alpha , nuclear translocation of NF-kappaB, and expression of type II nitric oxide synthase
Sheu et al., Food Chem Toxicol 2006 (Glomerulonephritis) : Moreover, addition of 100 microM CLA significantly diminished LPS-IFN-gamma induced protein degradation of inhibitor kappaB-alpha ( IkappaB-alpha ) and the protein expression of phosphorylated IkappaB-alpha in the cytosolic fraction as well as nuclear p65 expression ( P < 0.05 )
Sun et al., J Surg Res 2005 : NF-kappaB activation and IkappaBalpha degradation induced by LPS were attenuated by HSP
Yanaka et al., Int J Mol Med 2005 : Vitamin B6 inhibited LPS induced nuclear translocation of the NF-kappaB, the proinflammatory transcription factor, with reduction of the extent of LPS induced IkappaBalpha degradation in RAW cells ... Although vitamin B6 did not affect cellular proteasome activity, in vitro phosphorylation analysis with glutathione S-transferase fused IkappaBalpha has shown that vitamin B6 suppressed LPS induced IkappaB kinase activation
Hseu et al., Int Immunopharmacol 2005 : Furthermore, A. camphorata blocks the IkappaB-alpha degradation induced by LPS
Mandrekar et al., Alcohol Clin Exp Res 2006 (Atherosclerosis) : Further, LPS induced IkappaBalpha degradation was not affected by acute alcohol consumption indicating an IkappaBalpha independent mechanism, as observed earlier in the in vitro acute alcohol studies
Suh et al., Arch Biochem Biophys 2006 : These activities are associated with the down-regulation of inhibitor kappaB (IkappaB) kinase ( IKK ) activity by OC ( 6 microM ), thus inhibiting LPS induced phosphorylation as well as the degradation of IkappaBalpha
Eun et al., Dig Dis Sci 2006 (Colitis) : PPARgamma ligand delayed LPS induced IkappaBalpha degradation
Shin et al., Int Immunopharmacol 2006 : NFD-37 exhibited a dose dependent inhibitory effect on LPS induced phosphorylation of inhibitory kappaB alpha protein ( IkappaB alpha ), and subsequently inhibited LPS induced IkappaB alpha degradation as well as NF-kappaB transcriptional activity
Kim et al., Biochem Biophys Res Commun 2006 (Inflammation) : Further study indicated that LPS induced degradation and phosphorylation of Ikappa-Balpha , along with DNA binding of NF-kappaB, was significantly inhibited by rLE exposure in RAW264.7 cells
Aktan et al., Planta Med 2006 : This occurred at the transcriptional level, since the gingerol compounds decreased LPS induced IkappaB-alpha degradation, prevented nuclear translocation of NF-kappaB p65 and reduced NF-kappaB activity in a concentration dependent manner
Lee et al., Planta Med 2006 : Additionally, pinosylvin was found to inhibit the LPS induced phosphorylation and degradation of IkappaB alpha in THP-1 cells
Kim et al., Toxicology 2006 : SB203580 inhibited LPS induced serine phosphorylation, degradation of IkappaB-alpha , and tyrosine phosphorylation of p65 NF-kappaB
Jang et al., Eur J Pharmacol 2006 : These results suggest that tanshinone IIA may inhibit LPS induced IkappaB alpha degradation and NF-kappaB activation via suppression of the NIK-IKK pathway as well as the MAPKs ( p38, ERK1/2, and JNK ) pathway in RAW 264.7 cells and these properties may provide a potential mechanism that explains the anti-inflammatory activity of tanshinone IIA
Pan et al., Biochem Pharmacol 2006 (Cocarcinogenesis...) : Furthermore, acacetin inhibited LPS induced phosphorylation as well as degradation of I kappa B alpha
Ramana et al., J Biol Chem 2006 : The AR inhibition or ablation significantly attenuated LPS induced activation of protein kinase C ( PKC ) and phospholipase C (PLC), nuclear translocation of NF-kappaB, and phosphorylation and proteolytic degradation of IkappaBalpha in macrophages
Parsons et al., J Leukoc Biol 2006 (Inflammation) : Cultured Fps/Fes null macrophages also displayed prolonged LPS induced degradation of IkappaB-alpha , increased phosphorylation of the p65 subunit of NF-kappaB, and defective TLR4 internalization, compared with wild-type macrophages
Cappello et al., Arterioscler Thromb Vasc Biol 2007 (Atherosclerosis) : The presence of ozLDL led to a decrease in LPS induced IkappaB alpha proteolysis and a reduction of kappaB dependent transcription/target-gene expression
Hassan et al., BMC cancer 2006 (Neuroblastoma) : However, LPS did not significantly induce NF-kappaB activation in NB-1 cells although it slightly degraded IkappaB-alpha
Cheng et al., Biochem Pharmacol 2007 (Endotoxemia...) : Baicalein inhibited iNOS protein production, and suppressed LPS induced degradation of IkappaBalpha , the formation of a nuclear factor kappa B (NF-kappaB)-DNA complex and NF-kappaB dependent reporter gene expression
Kang et al., Pharmacol Res 2007 : Furthermore, EAAD suppressed LPS induced phosphorylation of p38 MAPK and extracellular-signal regulated kinases 1/2 ( ERK1/2 ), I-kappaBalpha degradation, and NF-kappaB activation in RAW 264.7 cells
Lin et al., Cell Signal 2007 : LPS stimulated translocation of NF-kappaB into the nucleus and degradation of IkappaB-alpha was blocked by helenalin, U0126, SB202190, or SP600125
Schwab et al., Mol Immunol 2007 : Cytosolic expression of I kappa B alpha protein was reduced by butyrate, and TNFalpha but not by LPS
Usluoglu et al., Eur J Immunol 2007 : RIP2 mediates LPS induced p38 and IkappaBalpha signaling including IL-12 p40 expression in human monocyte derived dendritic cells
Jeong et al., Int J Neurosci 2007 : YHT also inhibited LPS induced activation of NF-kappaB and degradation of IkappaBalpha
Hou et al., Biochem Pharmacol 2007 : Immunochemical analysis revealed that PDG suppressed LPS induced phosphorylation and degradation of IkappaBalpha , and subsequent nuclear translocation of p65
Zhou et al., Inflamm Res 2007 : ( 3 ) LXA ( 4 ) restrained LPS induced IkappaBalpha degradation, NF-kappaB translocation, and NF-kappaB transcriptional activity
Lin et al., J Leukoc Biol 2007 : PlGF was also able to inhibit LPS induced IkappaBalpha phosphorylation and NF-kappaB activity
von Bülow et al., J Immunol 2007 (MAP Kinase Signaling System) : By this mechanism, zinc suppressed LPS induced activation of IkappaB kinase beta (IKKbeta) and NF-kappaB, and subsequent TNF-alpha production
Moulakakis et al., J Immunol 2007 (Inflammation) : In this study, we show that in AM and PBMC from IkappaB-alpha knockout/IkappaB-beta knockin mice, SP-A fails to inhibit LPS induced TNF-alpha production and p65 nuclear translocation, confirming a critical role for IkappaB-alpha in SP-A mediated LPS inhibition
Choi et al., J Med Food 2007 : Furthermore, MFWE suppressed the LPS induced phosphorylations of p38 MAPK and extracellular signal regulated kinase MAPK, as well as IkappaBalpha degradation and NF-kappaB activation
Suh et al., Arch Biochem Biophys 2007 : EMSA and Western blot analysis revealed that OA drastically reduced NF-kappaB translocation by the inhibition effects of LPS induced phosphorylation of IkappaBalpha
Kanayama et al., J Endotoxin Res 2007 : Here, we show that bikunin : ( i ) blocks LPS induced secretion of pro-inflammatory cytokines, including TNF-alpha and IL-1beta, in a dose dependent manner ; ( ii ) has an inhibitory effect on cytokine production at a concentration of 0.2 microM, reaching 65 % inhibition at the highest doses of bikunin tested ( 5 microM ) ; ( iii ) has the suppressive capacity of ERK1/2 and p38 signaling pathways ; and ( iv ) inhibited sequentially the LPS induced phosphorylation of IkappaB-alpha , degradation of IkappaB-alpha, and nuclear translocation of NF-kappaB
Luyendyk et al., J Immunol 2008 (MAP Kinase Signaling System) : Modulating the PI3K-Akt pathway did not affect LPS induced degradation of IkappaBalpha or NF-kappaB nuclear translocation
Abboud et al., Shock 2008 (Anoxia...) : The addition of HSCM also attenuated LPS mediated NF-kappaB-DNA binding and IkappaBalpha degradation
Wiser et al., Free Radic Biol Med 2008 (Asthma) : We further obtained human monocytes from a subset of these volunteers and treated them ex vivo with gammaT, alphaT, gamma-CEHC, and alpha-CEHC and assessed their actions on LPS induced degradation of IkappaBalpha and JNK signaling and ROS generation
Zmijewski et al., Am J Respir Crit Care Med 2008 (Respiratory Distress Syndrome, Adult) : Inhibition of complex I with either rotenone or the antihyperglycemic agent metformin was associated with increased intracellular levels of both superoxide and hydrogen peroxide, as well as inhibition of LPS induced I kappaB-alpha degradation, NF-kappaB nuclear accumulation, and proinflammatory cytokine production
Jang et al., Proc Natl Acad Sci U S A 2008 (Encephalitis) : Consistent with this finding, luteolin did not inhibit LPS induced degradation of IkappaB-alpha but inhibited JNK phosphorylation
Song et al., Acta Otolaryngol 2008 (Otitis Media) : LPS induced IL-8 production was determined by enzyme linked immunosorbent assay ( ELISA ), and LPS induced IkappaB-alpha degradation was followed by Western blot analysis ... Furthermore, LPS induced IkappaB-alpha degradation was suppressed by the CAPE pretreatment
Turnbull et al., Journal of inflammation (London, England) 2008 : LPS stimulated loss of cytoplasmic IkappaBalpha and nuclear translocation of the p65 NF-kappaB subunit was inhibited by the active NO-furoxans
Jin et al., Phytother Res 2008 : UA also inhibited LPS mediated I-kappaBalpha degradation
Sag et al., J Immunol 2008 (Inflammation) : In addition, we found that AMPK negatively regulated LPS induced IkappaB-alpha degradation and positively regulated Akt activation, accompanied by inhibition of glycogen synthase kinase beta and activation of CREB
Zou et al., Am J Physiol Heart Circ Physiol 2009 (Disease Models, Animal...) : LPS ( 2 microg/ml ) increased the levels of IkappaB-alpha phosphorylation, TNF-alpha, ICAM-1, and NF-kappaB in primary cultured cardiomyocytes, which was significantly attenuated by glucosamine treatment and overexpression of O-GlcNAc transferase ; both interventions also significantly increased O-GlcNAc levels ... In contrast, the transfection of neonatal rat ventricular myocytes with OGT small interfering RNA decreased O-GlcNAc transferase and O-GlcNAc levels and enhanced the LPS induced increase in IkappaB-alpha phosphorylation
Walton et al., Am J Physiol Gastrointest Liver Physiol 2009 : LPS induced rapid phosphorylation of NF-kappaB p65, Akt, and p38 MAPK and degradation of Ikappa-Balpha
Prawan et al., Chem Biol Interact 2009 (Inflammation) : Immunoblotting showed that ITCs suppressed LPS induced phosphorylation and degradation of IkappaB alpha and decreased nuclear translocation of p65
Kizaki et al., Mol Immunol 2009 : On the other hand, depletion of beta ( 2 ) AR or beta-arrestin 2 expression by siRNA decreased cytoplasmic IkappaB alpha and abrogated late-phase IkappaB alpha degradation and NF-kappaB activation in response to LPS
Zmijewski et al., Am J Physiol Lung Cell Mol Physiol 2009 (Lung Injury) : Inhibition of complex III activity reduced LPS induced degradation of IkappaB-alpha , nuclear accumulation of NF-kappaB, and proinflammatory cytokine production ... The effects of antimycin A or myxothiazol appeared to be dependent on generation of H ( 2 ) O ( 2 ) since addition of pegylated catalase to neutrophils restored LPS mediated IkappaB-alpha degradation and production of proinflammatory cytokines
Trakala et al., Eur J Immunol 2009 (Disease Susceptibility...) : LPS stimulation of p21-deficient macrophages led to increased IkappaBalpha kinase activity, and increased IkappaBalpha phosphorylation and degradation, resulting in elevated NF-kappaB activity
Joo et al., PloS one 2009 (Colitis...) : In vitro, TLE blocked LPS induced IkappaBalpha degradation, RelA translocation, NF-kappaB transcriptional activity and MIP-2 mRNA accumulation in IEC-18 cells
Wu et al., Inflamm Res 2009 : Preincubation of RPMCs with Ang II significantly increased lipopolysaccharide (LPS) induced phospho-IkappaBalpha and phospho-NF-kappaB p65 protein ( P < 0.05 vs. LPS alone ) and CD40, TNF-alpha, and IL-6 mRNA levels ( P < 0.05 vs. LPS alone )
Okada et al., Int J Exp Pathol 2009 : The three species of Bifidobacterium significantly inhibited phosphorylation of I kappaB-alpha induced by LPS
Bumrungpert et al., J Nutr 2009 (Inflammation...) : Furthermore, gamma-MG but not alpha-MG attenuated LPS mediated IkappaB-alpha degradation and nuclear factor-kappaB (NF-kappaB) activity
Sánchez-Lemus et al., Brain Behav Immun 2009 (Inflammation) : The ARB reduced LPS induced pituitary gene expression of IL-6, LIF, iNOS, COX-2 and IkappaB-alpha ; and prevented LPS induced increase of nNOS/eNOS activity
Yoo et al., Neuroreport 2009 : In addition, nargenicin blocked LPS induced degradation of IkappaB-alpha , indicating that the initial molecular target of nargenicin is the transcription factor nuclear factor-kappaB
Lee et al., Exp Mol Med 2009 : Tat-SOD inhibited LPS induced NF-kappaB DNA binding activity, IkappaBalpha degradation and activation of MAP kinases
Taya et al., Arch Oral Biol 2009 : Furthermore, trehalose inhibited LPS induced reduction of IkappaB-alpha
Watanabe et al., Am J Physiol Gastrointest Liver Physiol 2009 (Disease Models, Animal...) : Western blot analyses showed that both L-lactic acid and LcS culture supernatants suppressed phosphorylation and degradation of I-kappaB-alpha induced by LPS without affecting expression of TLR4
Yang et al., J Neuroimmunol 2009 : Consistent with this finding, BE inhibited LPS induced IkappaBalpha degradation, NF-kappaB nuclear translocation, and also Akt, c-Jun NH2-terminal kinase (JNK) phosphorylation
Chang et al., J Pharmacol Sci 2009 : Furthermore, the LPS induced degradation of cytosolic IkappaB-alpha and activation of nuclear transcription factor-kappaB ( NF-kappaB ) were also inhibited by FC-77
Chang et al., Inflammation 2010 (Inflammation) : Both GLA and LA treatments dramatically inhibited LPS induced IkappaB-alpha degradation, IkappaB-alpha phosphorylation, and nuclear p65 protein expression
Chhikara et al., PloS one 2009 (MAP Kinase Signaling System) : CO was subsequently shown to block LPS induced phosphorylation and degradation of IkappaBalpha in human monocytes, thereby inhibiting NF-kappaB signal transduction
Raymond et al., Biochem Pharmacol 2010 : However, neither LT nor specific p-38 inhibitor interfered with LPS induced IkappaBalpha degradation or NF-kappaB nuclear translocation in AMs
Yoon et al., Food Chem Toxicol 2010 : Furthermore, AFE inhibited the LPS induced phosphorylation and degradation of Ikappa-B-alpha , which is required for the nuclear translocations of the p50 and p65 NF-kappaB subunits in RAW 264.7 cells
Wang et al., FASEB J 2010 (Hypercapnia) : Notably, hypercapnia did not affect LPS induced degradation of IkappaB alpha , nuclear translocation of RelA/p65, or activation of mitogen activated protein kinases, but it did block IL-6 promoter-driven luciferase activity in mouse RAW 264.7 macrophages
He et al., Chin Med J (Engl) 2010 : However, DEX had little effect on LPS induced NF-kappaB activation, and partially impaired LPS induced I-kappaBalpha degradation
Lee et al., Eur J Pharmacol 2010 (MAP Kinase Signaling System) : Pretreatment of eupatolide inhibited LPS induced phosphorylation and degradation of I kappaB alpha , and phosphorylation of RelA/p65 on Ser-536 as well as the activation of mitogen activated protein kinases ( MAPKs ) and Akt in LPS stimulated RAW264.7 cells
Sánchez et al., Endocrinology 2010 : The LPS induced increase in Ikappa-Balpha in the pars tuberalis was associated with increased TSHbeta gene expression in this tissue, but cAMP response element binding protein ( CREB ) phosphorylation was observed only in a subset of alpha tanycytes ... To determine whether signaling through TNF and/or nuclear factor-kappaB contributes to bacterial lipopolysaccharide (LPS) induced activation of type 2 iodothyronine deiodinase ( D2 ) in tanycytes lining the floor and infralateral walls of the third ventricle, the effect of a TNF antagonist on D2 gene expression and LPS induced Ikappa-Balpha expression in tanycytes were studied
Wu et al., J Sci Food Agric 2010 (Inflammation) : Moreover, LPS induced IkappaB-alpha phosphorylation and nuclear p65 protein expression as well as nuclear factor-kappaB (NF-kappaB) nuclear protein-DNA binding affinity and reporter gene activity were dramatically inhibited by aerial parts of CSEE
Hsieh et al., Phytomedicine 2011 (Bone Resorption...) : On osteoclasts, icariin suppresses LPS mediated activation of the p38 and JNK ; while on the osteoblasts, icariin reduced the LPS induced activation of ERK1/2 and I-kappa-B-alpha ( I?Ba ), but increased the activation of p38
Into et al., Cell Immunol 2010 (Bacterial Infections...) : LL-37 potently suppressed LPS induced gene expression of IL6, IL8 and CXCL10 and intracellular signaling events, degradation of IRAK-1 and IkappaBalpha and phosphorylation of p38 MAPK and IRF3, indicating that the LPS neutralizing activity is also exerted in HGFs. LL-37 also suppressed the expression of IL6, IL8 and CXCL10 induced by the TLR3 ligand poly ( I:C )
Yamashiro et al., Biochem Biophys Res Commun 2010 : ShRNA against TRPV2 inhibited the LPS induced TNFalpha and IL-6 production as well as IkappaBalpha degradation
Zerfaoui et al., J Immunol 2010 : PARP-1 was dispensable for LPS induced IkappaBalpha phosphorylation and subsequent degradation but was required for p65 NF-kappaB phosphorylation
Burke et al., Br J Surg 2010 (Colonic Neoplasms) : LPS treatment of fibroblasts induced IkappaBalpha degradation, enhanced NFkappaB promoter activity and increased collagen contraction
Oeth et al., Blood 1995 : Furthermore, sodium salicylate blocked the LPS induced proteolytic degradation of I kappa B alpha , which prevented the nuclear translocation of c-Rel/p65 heterodimers
Kuno et al., J Biol Chem 1995 : LPS stimulation transiently enhanced the I kappa B alpha bound kinase activity in THP-1 cells ... Moreover, we show that the peptide, corresponding to the C-terminal acidic domain of I kappa B alpha, blocked the LPS induced NF-kappa B activation as well as inducible phosphorylation of endogenous I kappa B alpha in a cell-free system using THP-1 cells
Rice et al., EMBO J 1993 : In this report we show that lipopolysaccharide (LPS) induced activation of B or pre-B cells results in loss of I kappa B alpha from NF-kappa B complexes in vivo
Milligan et al., Arch Biochem Biophys 1996 : LPS stimulated rapid proteolytic degradation of IkappaB-alpha which was inhibited by approximately 50 % in the presence of calpain inhibitor I
Zhang et al., Arterioscler Thromb Vasc Biol 1997 : NG-R1 inhibited the LPS induced degradation of I kappa B-alpha and superinduced LPS induced I kappa B-alpha mRNA, indicating that the effect of NG-R1 is not restricted to endothelial cells and is at least in part mediated by interference with the NF-kappa B/I kappa B-alpha pathway
Chen et al., Arch Biochem Biophys 1997 : Both silica and lipopolysaccharide (LPS) induce a rapid degradation of I kappa B alpha , an intracellular inhibitor of the nuclear factor (NF)-kappa B transcription factor ... In this report, we demonstrate that MG132, a relatively specific proteasome inhibitor, is capable of suppressing LPS induced I kappa B alpha degradation and NF-kappa B activation in mouse macrophage line RAW 264.7 cells, but is unable to influence the same induction produced by silica ... In contrast, the lysosome inhibitor chloroquine has little effect on I kappa B alpha degradation induced by either silica or LPS ... In contrast, no inhibition of LPS induced I kappa B alpha degradation and NF-kappa B activation was observed by the overexpression of calpastatin ... This suggests that calpain contributes to silica induced I kappa B alpha degradation and NF-kappa B activation but not to LPS induced I kappa B alpha degradation and NF-kappa B activation
Jeong et al., Immunology 1997 : In RAW 264.7 cells TPCK and TLCK significantly suppressed LPS induced increase in TNF-alpha mRNA, induction of nuclear kappa B-binding activity and degradation of I kappa B alpha
Mustafa et al., J Biol Chem 1998 (Shock, Septic) : In addition, the LPS dependent degradation of IkappaBalpha was not observed in forskolin treated cells ; the levels of the p65 subunit of NF-kappaB were minimal in the nucleus at the same time ... Also, we observed that forskolin induced transcription of the IkappaBalpha gene in a time dependent manner and in addition up-regulated LPS induced IkappaBalpha mRNA levels
Haas et al., J Leukoc Biol 1998 : Exposure of human monocytic THP-1 cells to ALLN and Mu873 prevented the LPS induced degradation of IkappaB-alpha and -beta, as did the more potent proteasome inhibitor, PSI, whereas several calpain inhibitors were ineffective ... In primary human blood monocytes ALLN also prevented the LPS induced degradation of IkappaB-alpha and -beta, efficiently blocked the production of TNF and, to a lesser extent, IL-1beta, whereas that of IL-8 was not inhibited
Greenberg et al., Alcohol 1998 : ETOH does not directly inhibit NFkappaB or AP-1, in vivo, but rather inhibits LPS induced activation of the MEKK/MAP kinase system and inhibition of inhibitory protein IkappaBalpha required for formation of AP-1 and NFkappaB, respectively
Feinstein et al., Nitric Oxide 1997 : In parental C6 cells, the presence of LPS plus CM caused a rapid ( within 30 min ) decrease in inhibitory IkappaB-alpha protein levels, and this loss was abolished by prior heat shock of the cells
Shames et al., Am J Physiol 1998 : In saline pretreated rats, LPS caused a rapid decrease in myocardial IkappaB-alpha protein levels, activation of NF-kappaB, and increased TNF-alpha production
Zen et al., Exp Cell Res 1998 : LPS induced IkappaBalpha degradation at 30-60 min after treatment, but did not induce IkappaBbeta degradation up to 120 min ... In contrast, TNF-alpha rapidly induced IkappaBalpha degradation within 5 min and IkappaBbeta degradation within 15 min. Cycloheximide did not prevent LPS induced IkappaBalpha degradation, indicating that newly synthesized proteins induced by LPS were not involved in LPS stimulated IkappaBalpha degradation ... LPS induced IkappaBalpha degradation was inhibited by ALLN, confirming that ALLN inhibits NF-kappaB activation by preventing IkappaBalpha degradation ... Of note, HMA also inhibited LPS induced IkappaBalpha degradation ... We conclude that in HUVEC, LPS induces NF-kappaB dependent genes through degradation of IkappaBalpha , not IkappaBbeta, and propose that this degradation is induced in part by HMA-sensitive kinase ( s ) upstream of IkappaBalpha
Chen et al., J Biol Chem 1998 : LPS induced degradation of IkappaB-alpha as well as the phosphorylation of IkappaB-alpha were also increased by UTP
St-Denis et al., J Biol Chem 1998 : Moreover, LPS induced phosphorylation and degradation of IkappaBalpha , NF-kappaB activation, as well as p38 mitogen activated protein kinase and Jun N-terminal kinase phosphorylation, were not affected by DN PKC-alpha overexpression
Shames et al., Shock 1998 (Endotoxemia) : The purpose of this study was to determine the effect of IL-10 on lipopolysaccharide (LPS) induced human monocyte TNF-alpha production, NF-kappaB activation, and IkappaB-alpha degradation ... IL-10 pretreatment prevented LPS induced decreases in IkappaB-alpha protein levels and attenuated NF-kappaB DNA binding
Manna et al., J Immunol 1999 : Within minutes, LPS through CD14 induced the activation of NF-kappaB, degradation of IkappaBalpha ( inhibitory subunit of NF-kappaB ) and IkappaBbeta, and nuclear translocation of p65