Free radical research 2005,
PMID: 16087481
Qiao, Shanlou; Li, Weihua; Tsubouchi, Ryoko; Haneda, Miyako; Murakami, Keiko; Takeuchi, Fumio; Nisimoto, Yukio; Yoshino, Masataka
Antioxidant action of Rosmarinic acid (Ros A), a natural phenolic ingredient in many Lamiaceae herbs such as Perilla frutescens, sage, basil and mint, was analyzed in relation to the Ikappa-B activation in RAW264.7 macrophages. Ros A inhibited nitric oxide (NO) production and inducible nitric oxide synthase (iNOS) protein synthesis induced by lipopolysaccharide (LPS), and also effectively suppressed phorbol 12-myristate 13-acetate (PMA)-induced superoxide production in RAW264.7 macrophages in a dose-dependent manner. Peroxynitrite-induced formation of 3-nitrotyrosine in bovine serum albumin and RAW264.7 macrophages were also inhibited by Ros A. Moreover, Western blot analysis demonstrated that LPS-induced phosphorylation of Ikappa-Balpha was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite-mediated damage, and as a potent inhibitor of superoxide and NO synthesis; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Ikappa-Balpha.
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Text Mining Data
inducible nitric oxide synthase (iNOS) → lipopolysaccharide (LPS): "
Ros A inhibited nitric oxide ( NO ) production and
inducible nitric oxide synthase (iNOS) protein synthesis
induced by
lipopolysaccharide (LPS) , and also effectively suppressed phorbol 12-myristate 13-acetate ( PMA ) -induced superoxide production in RAW264.7 macrophages in a dose dependent manner
"
Ikappa-Balpha → LPS: "
Moreover, Western blot analysis demonstrated that LPS induced phosphorylation of Ikappa-Balpha was abolished by Ros A. Ros A can act as an effective protector against peroxynitrite mediated damage, and as a potent inhibitor of superoxide and NO synthesis ; the inhibition of the formation of reactive oxygen and nitrogen species are partly based on its ability to inhibit the serine phosphorylation of Ikappa-Balpha
"
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