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EGF — SMAD3
Text-mined interactions from Literome
Kretzschmar et al., Genes Dev 1999
(Cell Transformation, Neoplastic...) :
EGF , which is weaker than oncogenic mutations at activating Ras,
induces a less extensive phosphorylation and cytoplasmic retention of Smad2 and
Smad3
Peron et al., J Biol Chem 2001
:
Whereas inhibition of JNK had no effect, inhibition of the phosphatidylinositol-3 ' kinase ( PI3-kinase ) pathway by LY294002 or by expression of a dominant negative mutant of PI3-kinase reduced
EGF/TGF-beta induced
Smad3 transcriptional activity ... The
EGF/TGF-beta induced activation of
Smad3 correlated with PI3-kinase and p38 dependent but not JNK dependent phosphorylation of c-Jun
Esguerra et al., Development 2007
:
Stimulation of
Alk4/EGF-CFC receptor complexes by Nodal
activates Smad2/3 , leading to left sided expression of target genes that promote asymmetric placement of certain internal organs
Yang et al., Am J Physiol Lung Cell Mol Physiol 2008
(MAP Kinase Signaling System) :
We show that
EGF does not
prevent the TGF-beta induced nuclear accumulation of
Smad2/3 ; rather, EGF stabilizes the short lived Smad transcriptional corepressor TG-interacting factor (TGIF) via EGFR/Mek/Erk mediated phosphorylation of TGIF
Diamond et al., J Cell Sci 2008
(Carcinoma, Squamous Cell...) :
Mechanistically,
EGF enhanced Smad phosphorylation in the linker region, and
attenuated TGFbeta1 mediated phosphorylation of
Smad at the C-terminus, localization of Smad to the nucleus as well as Smad-driven promoter activity exclusively in oral keratinocytes but not in OSCC cells ... The
effect of
EGF on TGFbeta1 mediated
Smad-driven promoter activity and N-cadherin expression was reversed when activation of ERK1/2 was blocked
Matsuura et al., J Biol Chem 2010
(Neoplasm Invasiveness...) :
Although epidermal growth factor also induces phosphorylation of threonine 179 and other residues in the Smad3 linker region the same as TGF-beta, Pin1 is unable to bind to the
epidermal growth factor stimulated
Smad3
Kim et al., Cancer Lett 2012
(Breast Neoplasms) :
EGF induced the phosphorylation of EGFR,
smad3 , ERK, and JNK, and MMP-9 expression was decreased by the EGFR inhibitor, AG1478 ... Interestingly,
EGF induced
smad3 phosphorylation was completely blocked by smad7 over-expression, but not the phosphorylation of ERK and JNK ... This study showed that
EGF induced
smad3 phosphorylation mediates the induction of MMP-9, whereas smad7 inhibits TGF-ß1 as well as the EGF signaling pathway in SKBR3 cells
Iwayama et al., Nephron extra 2011
:
Inhibition of p38MAPK but not of ERK abolished the protective
effect of
EGF on CyA induced nuclear translocation of
p-Smad2/3 and apoptosis
de Caestecker et al., Genes Dev 1998
:
We demonstrate here that HGF and
EGF , which signal through RTKs, can also mediate SMAD dependent reporter gene activation and
induce rapid phosphorylation of endogenous
SMAD proteins by kinase ( s ) downstream of MEK1