bigGenePred Track Format

The bigGenePred format stores positional annotations for collections of exons in a compressed format, similar to how BED files are compressed into bigBeds. The bigGenePred format includes 8 additional fields that contain details about coding frames, annotation status, and other gene-specific information. This is commonly used in the Browser to display start codons, stop codons, and amino acid translations.

Before compression, bigGenePred files can be described as bed12+8 files. bigGenePred files can be created using the program bedToBigBed, run with the -as option to pull in a special autoSql (.as) file that defines the extra fields of the bigGenePred.

Much like bigBed, bigGenePred files are in an indexed binary format. The advantage of using a binary format is that only the portions of the file needed to display a particular region are read, allowing for much faster performance when working with large data sets. As with all big* files, bigGenePred files must be hosted on a web-accessible server (http, https, or ftp) to be displayed. For more information on finding a hosting location for your bigGenePred files, please see the hosting section of the Track Hub Help documentation.

bigGenePred format description

The following autoSql definition specifies bigGenePred gene prediction files. This definition, contained in the file bigGenePred.as, is pulled in when the bedToBigBed utility is run with the -as=bigGenePred.as option.

table bigGenePred
"bigGenePred gene models"
    (
    string chrom;       	"Reference sequence chromosome or scaffold"
    uint   chromStart;  	"Start position in chromosome" 
    uint   chromEnd;    	"End position in chromosome"
    string name;        	"Name or ID of item, ideally both human-readable and unique"
    uint score;         	"Score (0-1000)"
    char[1] strand;     	"+ or - for strand"
    uint thickStart;    	"Start of where display should be thick (start codon)"
    uint thickEnd;      	"End of where display should be thick (stop codon)"
    uint reserved;       	"RGB value (use R,G,B string in input file)"
    int blockCount;     	"Number of blocks"
    int[blockCount] blockSizes; "Comma separated list of block sizes"
    int[blockCount] chromStarts;"Start positions relative to chromStart"
    string name2;       	"Alternative/human readable name"
    string cdsStartStat; 	"Status of CDS start annotation (none, unknown, incomplete, or complete)"
    string cdsEndStat;   	"Status of CDS end annotation (none, unknown, incomplete, or complete)"
    int[blockCount] exonFrames; "Exon frame {0,1,2}, or -1 if no frame for exon"
    string type;        	"Transcript type"
    string geneName;    	"Primary identifier for gene"
    string geneName2;   	"Alternative/human-readable gene name"
    string geneType;    	"Gene type"
    )  

The following bed12+8 is an example of a pre-bigGenePred text file .

Creating a bigGenePred track from a bed12+8 file

Step 1. Format your pre-bigGenePred file. The first 12 fields of pre-bigGenePred files are described by the BED file format. Your file must also contain the 8 extra fields described in the autoSql file definition shown above: name2, cdsStartStat, cdsEndStat, exonFrames, type, geneName, geneName2, geneType. For example, you can use this bed12+8 input file, bigGenePred.txt. Your pre-bigGenePred file must be sorted first on the chrom field, and secondarily on the chromStart field. You can use the UNIX sort command to do this:

sort -k1,1 -k2,2n unsorted.bed > input.bed

Step 2. Download the bedToBigBed program from the binary utilities directory.

Step 3. Download the chrom.sizes file for your assembly from our downloads page (click on "Full data set" for your organism). For example, the hg38.chrom.sizes file for the hg38 database is located at http://hgdownload.soe.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes. Alternatively, you can use the fetchChromSizes script from the utilities directory.

Step 4. Create the bigGenePred file from your pre-bigGenePred file using the bedToBigBed utility command:

bedToBigBed -as=bigGenePred.as -type=bed12+8 bigGenePred.txt chrom.sizes myBigGenePred.bb

Step 5. Move the newly created bigGenePred file (myBigGenePred.bb) to a web-accessible http, https, or ftp location. See hosting section if necessary.

Step 6. Construct a custom track using a single track line. Any of the track attributes will be available for use on bigBed tracks. The basic version of the track line will look something like this:

track type=bigGenePred name="My Big GenePred" description="A Gene Set Built from Data from My Lab" bigDataUrl=http://myorg.edu/mylab/myBigGenePred.bb

Step 7. Paste this custom track line into the text box on the custom track page with your modified URL. Click and your track should load successfully. Then click to be taken to the Browser window with your custom track at the top. Note that there might not be data at all positions.

Examples

Example #1: Create a Custom Track

Create a bigGenePred custom track using the bigGenePred file located on the UCSC Genome Browser http server, bigGenePred.bb. This file contains data for the hg38 assembly.

  1. Construct a track line that references the hosted file:

    track type=bigGenePred name="bigGenePred Example One" description="A bigGenePred file" bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigGenePred.bb
  2. Paste the track line into the custom track page for the human assembly, hg38.
  3. Click the button.

Custom tracks can also be loaded via one URL line. The link below loads the same bigGenePred track and sets additional parameters in the URL:

http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&hgct_customText=track%20type=bigGenePred%20bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigGenePred.bb

After this example bigGenePred track is loaded in the Genome Browser, click on the track to change display from dense to pack, then click on a gene in the Browser's track display to view the gene details page. Note that the page offers links to translated protein, predicted mRNA, and genomic sequence.

Example #2: Display Amino Acids

In this example, you will configure the bigGenePred track loaded in Example #1 to display codons and amino acid numbering:

  1. On the bottom of the gene details page, click the "Go to User Track track controls" link.
  2. Change the "Color track by codons:" option from "OFF" to "genomic codons" and change the display mode to "full". Then click .
  3. Zoom to a region with track data, such as chr9:133,255,650-133,255,700, and note that the track now displays amino acids.
  4. Return to the track controls page and click the box next to "Show codon numbering", then click . The Browser tracks display will now show amino acid letters and numbering.

Alternatively, you can also add a parameter in the custom track line, baseColorDefault=genomicCodons, to set amino acids to display by default:

browser position chr10:67,884,600-67,884,900
track type=bigGenePred baseColorDefault=genomicCodons name="bigGenePred Example Two" description="A bigGenePred file" visibility=pack bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigGenePred.bb 

Paste the above into the hg38 custom track page to view an example of bigGenePred amino acid display at the beginning of the SIRT1 gene on chromosome 10.

An image of a track with codons colored

Example #3: Bed12+8 to BigGenePred

In this example, you will create your own bigGenePred file from an existing pre-bigGenePred input file, a bed12+8 file.

  1. Save the example bed12+8 input file to your computer, bigGenePred.txt.
  2. Download the bedToBigBed utility (Step 2, in the Creating a bigGenePred section above).
  3. Save the hg38.chrom.sizes text file to your computer. This file contains the chrom.sizes for the human hg38 assembly (Step 3, above).
  4. Save the autoSql file bigGenePred.as to your computer.
  5. Run the bedToBigBed utility to create the bigGenePred output file (step 4, above):
    bedToBigBed -type=bed12+8 -tab -as=bigGenePred.as bigGenePred.txt hg38.chrom.sizes bigGenePred.bb
  6. Place the newly created bigGenePred file (bigGenePred.bb) on a web-accessible server (Step 5, above).
  7. Construct a track line that points to the bigGenePred file (Step 6, above).
  8. Create the custom track on the human assembly hg38 (Dec. 2013), and view it in the Genome Browser (step 7, above).

Example #4: GTF (or GFF) to BigGenePred

In this example, you will convert a GTF file to bigGenePred using command line utilities. You will need gtfToGenePred, genePredTobigGenePred, and bedToBigBed. If you would like to convert a GFF file to bigGenePred, you will use gff3ToGenePred in place of the gtfToGenePred. You can download utilities from the utilities directory.

  1. Obtain a GTF file using the wget command. Skip this step if you already have a GTF or GFF file.
    wget http://genome.ucsc.edu/goldenPath/help/examples/bigGenePredExample4.gtf
  2. Convert the GTF file to genePred extended format using the gtfToGenePred command.
    gtfToGenePred -genePredExt bigGenePredExample4.gtf example4.genePred
    If you are converting a GFF file, use the gff3ToGenePred command.
    gff3ToGenePred yourFile.gff example4.genePred 
  3. Convert the genePred extended file to a pre-bigGenePred text file.
    genePredToBigGenePred example4.genePred bigGenePredEx4.txt
  4. Download a helper file that specifies column names.
    wget https://genome.ucsc.edu/goldenPath/help/examples/bigGenePred.as
  5. Convert your text bigGenePred to a binary indexed format. If you are not using hg38, you will need to replace the hg38.chrom.sizes file path with your organism's file path from the downloads directory under "Genome Sequence Files".
    bedToBigBed -type=bed12+8 -tab -as=bigGenePred.as bigGenePredEx4.txt http://hgdownload.soe.ucsc.edu/goldenPath/hg38/bigZips/hg38.chrom.sizes bigGenePredEx4.bb
  6. Put your binary indexed file in a web-accessible location. See the hosting section for more information.
  7. To view this example, you can click this into this Browser link. To view your own data, paste the link into your web browser and replace the URL after "bigDataUrl=" with a link to your own hosted data.
     http://genome.ucsc.edu/cgi-bin/hgTracks?db=hg38&position=chr19:44905790-44909388&hgct_customText=track%20type=bigGenePred%20bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigGenePredEx4.bb
    You can also add your data in the custom track management page. This allows you to set position, configuration options, and write a more complete desciption. If you want to see codons, you can right click, then click configure codon view or set this options using baseColorDefault=genomicCodons as is done below.
    browser position chr19:44905790-44909388 
    track type=bigGenePred baseColorDefault=genomicCodons name="bigGenePred Example Four" description="Ex4:BigGenePred Made from GTF" visibility=pack bigDataUrl=http://genome.ucsc.edu/goldenPath/help/examples/bigGenePredEx4.bb
    Once you are done, you should have a track on the Genome Browser like the one below.

An image of a bigGenePred track on the Browser

Example #5: Create a BigGenePred Track Hub

In this example, you will set up a Track Hub that displays bigGenePred data and uses one of the bigGenePred-specific settings to display gene codons. You can see a pre-built version of this hub by clicking this link.

  1. Make sure you have access to a web-hosted file location like GitHub, CyVerse, or an institutional website. This is where you will store your bigData files and configuration files. For more information, please visit the hosting section of our Track Hub help guide.
  2. Copy the text from the bigGenePred example hub files into identically named files on your hosted website. You will need a hub.txt and a genomes.txt file, along with a directory for each assembly you would like to visualize (hg38). This assembly directory stores your data files (bigGenePred.bb) and a trackDb.txt file which defines track settings, such as track name, description, and the bigGenePred setting for amino acid display (baseColorDefault genomicCodons). Visit the trackDb help page for more information about trackDb settings.
  3. Verify that you have the four minimum files, two of which in the hg38 directory:
    --hub.txt
    --genomes.txt
    --hg38
    ----trackDb.txt
    ----bigGenePred.bb
    You may also include a hub description page and a track description page, where you will find additional instructions.
  4. Copy the URL address of your hub.txt file and paste it into the text input box on the Track Hub page. For an example, you can paste the following link into the input box: http://genome.ucsc.edu/goldenPath/help/examples/hubExamples/hubBigGenePred/hub.txt. Clicking the button will connect to your track hub and navagate you to your assembly of interest. For more instructions on setting up Track Hubs, visit the Track Hub set-up page.
  5. An image of a bigGenePred track hub on the Browser

Sharing your data with others

If you would like to share your bigGenePred data track with a colleague, learn how to create a URL link to your data by looking at Example #6 on the custom track help page.

Extracting data from bigBed format

Because the bigGenePred files are an extension of bigBed files, which are indexed binary files, it can be difficult to extract data from them. UCSC has developed the following programs to assist in working with bigBed formats, available from the binary utilities directory.

As with all UCSC Genome Browser programs, simply type the program name (with no parameters) at the command line to view the usage statement.

Troubleshooting

If you encounter an error when you run the bedToBigBed program, check your input file for data coordinates that extend past the end of the chromosome. If these are present, run the bedClip program (available here) to remove the problematic row(s) before running the bedToBigBed program.