Gene interactions and pathways from curated databases and text-mining

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ORC1 — ORC2

Pathways - manually collected, often from reviews:

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

Text-mined interactions from Literome

Palacios DeBeer et al., EMBO J 1999 : These data were consistent with a role for the ORC bound to the HMR-E silencer ACS in suppressing the function of neighboring ORC molecules capable of inhibiting silencing, and indicated that differences in ORC binding sites within HMR itself had profound effects on ORC function
Pasero et al., Genes Dev 1999 : The association of Dbf4p with a detergent-insoluble chromatin fraction in G ( 1 ) -phase nuclei requires ORC but not Cdc6p or Clb/Cdc28 kinase activity, and correlates with competence for initiation
Findeisen et al., Eur J Biochem 1999 : Cyclin A-dependent kinase activity affects chromatin binding of ORC , Cdc6, and MCM in egg extracts of Xenopus laevis ... We find that chromatin binding of ORC , Cdc6, and MCM proteins does not require cyclin dependent kinase activities ... High cyclin E kinase activity allows the binding of Xenopus ORC and Cdc6, but not MCM, to sperm chromatin, even though the kinase does not phosphorylate MCM directly
Kearsey et al., EMBO J 2000 : Chromatin binding of the fission yeast replication factor mcm4 occurs during anaphase and requires ORC and cdc18
Takayama et al., Genes Cells 2000 : Furthermore, ORC1 inhibited E-box dependent transcription activity of c-Myc by competitive binding to the C-terminal region of c-Myc with SNF5, a component of chromatin remodelling complex SNF/Swi1
Mizushima et al., Genes Dev 2000 : Cdc6p increases the DNA binding specificity of ORC by inhibiting non-specific DNA binding of ORC
Sun et al., EMBO J 2002 : Intriguingly, Xenopus ORC rapidly bound to hamster somatic chromatin in a Cdc6 dependent manner and was then released, concomitant with initiation of DNA replication
Schaarschmidt et al., Nucleic Acids Res 2002 : While the order of assembly -- Mcm binding follows ORC binding -- seems to be conserved in cycling mammalian cells in culture, it has not been determined whether mammalian Mcm proteins associate with ORC bearing chromatin sites
Van Hatten et al., J Cell Biol 2002 : Mini-chromosome maintenance (MCM)2-7 recruitment to origins in G1 requires origin recognition complex (ORC) , Cdt1, and Cdc6, and activation at G1/S requires MCM10 and the protein kinases Cdc7 and S-Cdk, which together recruit Cdc45, a putative MCM2-7 cofactor required for origin unwinding ... Mini-chromosome maintenance (MCM)2-7 recruitment to origins in G1 requires origin recognition complex (ORC) , Cdt1, and Cdc6, and activation at G1/S requires MCM10 and the protein kinases Cdc7 and S-Cdk, which together recruit Cdc45, a putative MCM2-7 cofactor required for origin unwinding
Takahashi et al., J Biol Chem 2004 : We therefore consider that ATP binding to Orc5p is involved in efficient ORC formation and that Orc4p is involved in this process
Prasanth et al., EMBO J 2004 : The initiation of DNA replication in S phase requires the prior assembly of an origin recognition complex (ORC) dependent pre-replicative complex on chromatin during G1 phase of the cell division cycle
Speck et al., Nature structural & molecular biology 2005 : ATPase dependent cooperative binding of ORC and Cdc6 to origin DNA
Randell et al., Mol Cell 2006 : The resulting protein-DNA assembly is called the prereplicative complex (pre-RC) , and its formation requires the origin recognition complex (ORC) , Cdc6, Cdt1, and ATP
Waga et al., J Biol Chem 2006 : Although geminin inhibited MCM loading, MCM interacted weakly but stoichiometrically with the plasmid in an ORC dependent manner, even in the presence of geminin ( with approximately 10 MCM2 molecules per plasmid )
Atanasiu et al., EMBO Rep 2006 : We now show that ORC is recruited selectively to the dyad symmetry ( DS ) region of OriP as a consequence of direct interactions with telomere repeat factor 2 (TRF2) and ORC1 ... We now show that ORC is recruited selectively to the dyad symmetry ( DS ) region of OriP as a consequence of direct interactions with telomere repeat factor 2 (TRF2) and ORC1
Speck et al., J Biol Chem 2007 : Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication ... Cdc6 ATPase activity regulates ORC x Cdc6 stability and the selection of specific DNA sequences as origins of DNA replication ... To initiate DNA replication ORC requires Cdc6 to target initiation to specific DNA sequences in chromosomes and with Cdt1 loads the ring shaped mini-chromosome maintenance (MCM) 2-7 DNA helicase component onto DNA ... Here we analyzed the role of the Cdc6 ATPase on ORC x Cdc6 complex stability in the presence or absence of specific DNA sequences ... Cdc6 ATPase is activated by ORC, regulates ORC x Cdc6 complex stability, and is suppressed by origin DNA
Asano et al., FEMS Yeast Res 2007 : Cdt1p neither bound to origin DNA and ATP nor affected ORC binding to origin DNA and ATP ... These results suggest that interaction of Cdt1p with ORC is involved in the formation of the prereplicative complex, rather than in regulation of the activity of ORC
Chen et al., Genes Dev 2007 : Replacing Orc6 with a fusion protein linking Cdt1 to the Orc6-CTD results in an ORC complex that loads Mcm2-7 onto DNA
Takehara et al., Biochem J 2008 : To reveal the role of this ATPase activity in Saccharomyces cerevisiae ( baker 's yeast ) ORC , we mutated the Orc1p sensor 1 and sensor 2 regions, which are important for ATPase activity in AAA ( + ) proteins
Lutzmann et al., Mol Cell 2008 : We demonstrate that MCM9 binds to chromatin in an ORC dependent manner and is required for the recruitment of the MCM2-7 helicase onto chromatin
Tempera et al., J Virol 2010 : Short hairpin RNA depletion of PARP1 enhances OriP replication activity and increases EBNA1, origin recognition complex 2 (ORC2) , and minichromosome maintenance complex (MCM) association with OriP ... Pharmacological inhibitors of PARP1 enhance OriP plasmid maintenance and increase EBNA1, ORC2 , and MCM3 occupancy at OriP
Lee et al., J Biol Chem 2012 : These results suggest that the phosphorylation of ORC2 dissociates ORC from chromatin and replication origins and inhibits binding of ORC to newly replicated DNA
Sun et al., Structure 2012 : Cdc6 induced conformational changes in ORC bound to origin DNA revealed by cryo-electron microscopy
Fernández-Cid et al., Mol Cell 2013 : Importantly, CDK dependent phosphorylation of ORC inhibits OCM establishment to ensure once per cell cycle replication
Ehrenhofer-Murray et al., Genetics 1997 : The role of Sas2 , an acetyltransferase homologue of Saccharomyces cerevisiae, in silencing and ORC function
Fox et al., Science 1997 : The requirement for ORC in silencing of HMR could be bypassed by tethering the Sir1 protein to the HMR-E silencer