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GRB2 — PTK2

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: Complex of GRB2-PTK2-SOS1 → PTK2 (increases, GRB2/PTK2/SOS1 Activity) Giancotti et al., Science 1999
  Evidence: FAK also combines with, and may activate, phosphoinositide 3-OH kinase (PI 3-kinase), either directly or through the Src kinase (13). Finally, there is evidence that Src phosphorylates FAK at Tyr925, creating a binding site for the complex of the adapter Grb2 and Ras guanosine 5'-triphosphate exchange factor mSOS (10). These interactions link FAK to signaling pathways that modify the cytoskeleton and activate mitogen-activated protein kinase (MAPK) cascades (Fig. 3A).
• BioCarta pten dependent cell cycle arrest and apoptosis: Integrin-beta/SHC/SOS-1/GRB2 complex (ITGB1-SHC1-SOS1-GRB2) → FAK (PTK2) (modification, activates)
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: None → FAK/Src-Yes-Fyn/GRB2/SOS1 complex (PTK2-GRB2-SOS1-YES1_SRC_FYN) (modification, collaborate)
  Furuhjelm et al., J Cell Sci 2003, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1997, Schlaepfer et al., J Biol Chem 1997
  Evidence: mutant phenotype
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: RRAS/GDP complex (RRAS) → FAK/Src-Yes-Fyn/GRB2/SOS1 complex (PTK2-GRB2-SOS1-YES1_SRC_FYN) (modification, collaborate)
  Furuhjelm et al., J Cell Sci 2003, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1997, Schlaepfer et al., J Biol Chem 1997
  Evidence: mutant phenotype
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → None (modification, activates) Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → HRAS/GTP complex (HRAS) (modification, activates) Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → HRAS/GDP complex (HRAS) (modification, activates) Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → None (modification, activates) Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: FAK/Src-Yes-Fyn/GRB2/SOS1 complex (PTK2-GRB2-SOS1-YES1_SRC_FYN) → FAK/Src-Yes-Fyn complex (PTK2-YES1_SRC_FYN) (modification, collaborate)
  Shen et al., FEBS Lett 2001, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1996, Schlaepfer et al., Mol Cell Biol 1997, Schlaepfer et al., J Biol Chem 1997, Li et al., J Biol Chem 1997, Schlaepfer et al., Mol Cell Biol 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: FAK/Src-Yes-Fyn/GRB2/SOS1 complex (PTK2-GRB2-SOS1-YES1_SRC_FYN) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Shen et al., FEBS Lett 2001, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1996, Schlaepfer et al., Mol Cell Biol 1997, Schlaepfer et al., J Biol Chem 1997, Li et al., J Biol Chem 1997, Schlaepfer et al., Mol Cell Biol 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by focal adhesion kinase: FAK/Src-Yes-Fyn complex (PTK2-YES1_SRC_FYN) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Shen et al., FEBS Lett 2001, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1996, Schlaepfer et al., Mol Cell Biol 1997, Schlaepfer et al., J Biol Chem 1997, Li et al., J Biol Chem 1997, Schlaepfer et al., Mol Cell Biol 1998
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → GRB2/SOS1 complex (GRB2-SOS1) (modification, collaborate)
  Lai et al., J Biol Chem 2000*, Ishibe et al., Mol Cell 2003, Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): FAK/GRB2/SOS1 complex (GRB2-SOS1-PTK2) → FAK (PTK2) (modification, collaborate) Lai et al., J Biol Chem 2000*, Ishibe et al., Mol Cell 2003, Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• NCI Pathway Database Signaling events mediated by Hepatocyte Growth Factor Receptor (c-Met): GRB2/SOS1 complex (GRB2-SOS1) → FAK (PTK2) (modification, collaborate) Lai et al., J Biol Chem 2000*, Ishibe et al., Mol Cell 2003, Chen et al., J Biol Chem 1998
  Evidence: assay, physical interaction
• Reactome Reaction: PTK2 → GRB2 (indirect_complex) Arold et al., Structure 2002, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1998
• Reactome Reaction: PTK2 → GRB2 (direct_complex)
• Reactome Reaction: PTK2 → GRB2 (reaction) Arold et al., Structure 2002, Cory et al., J Biol Chem 2002, Panicker et al., Frontiers in bioscience : a journal and virtual library 2003, Wu et al., J Biol Chem 2004, Probstmeier et al., J Neurochem 1989, Schlaepfer et al., Nature 1994, Schlaepfer et al., Mol Cell Biol 1998
• WikiPathways Focal Adhesion: PTK2 → GRB2 (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: PTK2 — GRB2 Kharbanda et al., Proc Natl Acad Sci U S A 1995*
• IRef Bind_translation Interaction: PTK2 — GRB2 (coimmunoprecipitation) Schlaepfer et al., J Biol Chem 1997
• IRef Bind_translation Interaction: GRB2 — PTK2 (coimmunoprecipitation) Li et al., J Biol Chem 1997
• IRef Bind_translation Interaction: PTK2 — GRB2 (affinity chromatography technology) Kharbanda et al., Proc Natl Acad Sci U S A 1995*
• IRef Bind_translation Interaction: PTK2 — GRB2 (coimmunoprecipitation) Kharbanda et al., Proc Natl Acad Sci U S A 1995*
• IRef Bind_translation Interaction: PTK2 — GRB2 (competition binding) Kharbanda et al., Proc Natl Acad Sci U S A 1995*
• IRef Biogrid Interaction: GRB2 — PTK2 (direct interaction, pull down) Arold et al., Structure 2002
• IRef Biogrid Interaction: GRB2 — PTK2 (physical association, affinity chromatography technology) Sieg et al., Nat Cell Biol 2000*
• IRef Biogrid Interaction: GRB2 — PTK2 (direct interaction, pull down) Hildebrand et al., Mol Cell Biol 1996*
• IRef Biogrid Interaction: GRB2 — PTK2 (direct interaction, pull down) Angers-Loustau et al., J Cell Biol 1999*
• IRef Biogrid Interaction: GRB2 — PTK2 (physical association, affinity chromatography technology) Messina et al., J Neurochem 2003*
• IRef Biogrid Interaction: GRB2 — PTK2 (direct interaction, two hybrid) Vinayagam et al., Science signaling 2011
• IRef Hprd Interaction: PTK2 — GRB2 (in vitro) Schlaepfer et al., Nature 1994
• IRef Hprd Interaction: PTK2 — GRB2 (in vivo) Schlaepfer et al., Nature 1994
• IRef Innatedb Interaction: PTK2 — GRB2 (unknown, -) Ohmori et al., Biochem J 2000*
• IRef Intact Interaction: PTK2 — GRB2 (physical association, two hybrid) Vinayagam et al., Science signaling 2011
• IRef Intact Interaction: PTK2 — GRB2 (association, pull down) Kharbanda et al., Proc Natl Acad Sci U S A 1995*
• IRef Intact Interaction: Complex of 23 proteins (association, anti bait coimmunoprecipitation) Thelemann et al., Mol Cell Proteomics 2005
• IRef Intact Interaction: Complex of 70 proteins (association, anti bait coimmunoprecipitation) Thelemann et al., Mol Cell Proteomics 2005
• IRef Ophid Interaction: PTK2 — GRB2 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005
• IRef Ophid Interaction: PTK2 — GRB2 (aggregation, confirmational text mining) Schlaepfer et al., Nature 1994

Text-mined interactions from Literome

Kharbanda et al., Proc Natl Acad Sci U S A 1995 : Stimulation of human monocytes with macrophage colony stimulating factor induces a Grb2 mediated association of the focal adhesion kinase pp125FAK and dynamin
Sieg et al., EMBO J 1998 : Significantly, repression of endogenous Src-family PTK activity by p50 ( csk ) overexpression inhibited FN-stimulated cell spreading, Pyk2 tyrosine phosphorylation, Grb2 binding to Shc, and ERK2 activation in the FAK- but not in FAK+ cells
Lebrun et al., J Biol Chem 1998 : In contrast, no change in mitogen activated protein kinase activity was observed, indicating that pp125(FAK) induced association between IRS-1 and growth factor receptor bound protein 2 does not affect the mitogen activated protein kinase pathway