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H2AFX — RAD50

Pathways - manually collected, often from reviews:

• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → ABRA1 (FAM175A) (modification, collaborate) Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) → ABRA1 (FAM175A) (modification, collaborate) Wang et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM complex (MRE11A-NBN-RAD50-ATM) → H2AX (H2AFX) (modification, activates) Burma et al., J Biol Chem 2001, Berkovich et al., Nat Cell Biol 2007, Rogakou et al., J Biol Chem 1998
  Evidence: mutant phenotype, assay
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Cortez et al., Science 1999, Li et al., Nature 2000, Xu et al., Mol Cell Biol 2001, Stewart et al., Nature 2003, Lou et al., J Biol Chem 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1 complex (MRE11A-NBN-RAD50-ATM-MDC1) → MRN complex/ATM/MDC1/H2AX complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1) (modification, collaborate)
  Goldberg et al., Nature 2003, Stewart et al., Nature 2003, Lukas et al., EMBO J 2004, Lou et al., Mol Cell 2006, Franco et al., Mol Cell 2006
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1 complex (MRE11A-NBN-RAD50-ATM-MDC1) → H2AX (H2AFX) (modification, collaborate) Goldberg et al., Nature 2003, Stewart et al., Nature 2003, Lukas et al., EMBO J 2004, Lou et al., Mol Cell 2006, Franco et al., Mol Cell 2006
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1) → H2AX (H2AFX) (modification, collaborate) Goldberg et al., Nature 2003, Stewart et al., Nature 2003, Lukas et al., EMBO J 2004, Lou et al., Mol Cell 2006, Franco et al., Mol Cell 2006
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1) → RAP80 (UIMC1) (modification, collaborate) Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1/RAP80 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A-UIMC1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: RAP80 (UIMC1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1/ABRA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1-FAM175A) (modification, collaborate)
  Wang et al., Science 2007, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → SMC1 (SMC1A) (modification, activates) Kim et al., Genes Dev 2002, Yazdi et al., Genes Dev 2002, Kitagawa et al., Genes Dev 2004
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → Artemis (DCLRE1C) (modification, activates) Zhang et al., Mol Cell Biol 2004, Riballo et al., Mol Cell 2004, Chen et al., Cancer Sci 2005
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → SMC3 (SMC3) (modification, activates) Luo et al., J Biol Chem 2008
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → 53BP1 (TP53BP1) (modification, collaborate) Rappold et al., J Cell Biol 2001, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007, Wilson et al., Cell cycle (Georgetown, Tex.) 2008, Lee et al., EMBO J 2010
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → MRN complex/ATM/MDC1/H2AX/RNF8 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8) (modification, collaborate)
  Rappold et al., J Cell Biol 2001, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007, Wilson et al., Cell cycle (Georgetown, Tex.) 2008, Lee et al., EMBO J 2010
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: 53BP1 (TP53BP1) → MRN complex/ATM/MDC1/H2AX/RNF8 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8) (modification, collaborate)
  Rappold et al., J Cell Biol 2001, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007, Wilson et al., Cell cycle (Georgetown, Tex.) 2008, Lee et al., EMBO J 2010
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → KAP1 (TRIM28) (modification, activates) Ziv et al., Nat Cell Biol 2006, Noon et al., Nat Cell Biol 2010
  Evidence: mutant phenotype, assay
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1/BRCA1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1-BRCA1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → CtBP/CtIP complex (RBBP8) (modification, collaborate) Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX/RNF8/53BP1 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8-TP53BP1) → BRCA1/CtBP/CtIP complex (BRCA1-CTBP1-RBBP8) (modification, collaborate)
  Li et al., Nature 2000, Xu et al., Cancer Res 2002, Stewart et al., Nature 2003, Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1) → RNF8 (RNF8) (modification, collaborate) Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1) → UBC13 (UBE2N) (modification, collaborate) Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: MRN complex/ATM/MDC1/H2AX complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1) → MRN complex/ATM/MDC1/H2AX/RNF8 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8) (modification, collaborate)
  Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: RNF8 (RNF8) → MRN complex/ATM/MDC1/H2AX/RNF8 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8) (modification, collaborate)
  Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• NCI Pathway Database ATM pathway: UBC13 (UBE2N) → MRN complex/ATM/MDC1/H2AX/RNF8 complex (MRE11A-NBN-RAD50-ATM-H2AFX-MDC1-RNF8) (modification, activates)
  Mailand et al., Cell 2007, Huen et al., Cell 2007, Kolas et al., Science 2007
  Evidence: mutant phenotype, assay, physical interaction
• Reactome Reaction: H2AFX → RAD50 (indirect_complex)
• Reactome Reaction: H2AFX → RAD50 (reaction)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: H2AFX — RAD50 (colocalization, imaging technique) Paull et al., Curr Biol 2000*
• IRef Biogrid Interaction: H2AFX — RAD50 (physical association, affinity chromatography technology) Lukas et al., EMBO J 2004
• IRef Dip Interaction: Complex of RAD50-H2AFX-MDC1-MRE11A (pull down) Cook et al., Nature 2009
• IRef Dip Interaction: H2AFX — RAD50 (physical association, pull down) Cook et al., Nature 2009
• IRef Intact Interaction: Complex of MRE11A-H2AFX-NBN-MDC1-RAD50 (association, pull down) Chapman et al., EMBO Rep 2008

Text-mined interactions from Literome

Furuta et al., J Biol Chem 2003 : Phosphorylation of histone H2AX and activation of Mre11, Rad50 , and Nbs1 in response to replication dependent DNA double-strand breaks induced by mammalian DNA topoisomerase I cleavage complexes
Amiard et al., Plant Cell 2010 (Chromosomal Instability) : We report the absence of ?-ray induced, ATM/ATR dependent histone H2AX phosphorylation in Arabidopsis thaliana rad50 and mre11 mutants, confirming that the MRN complex is required for H2AX phosphorylation by the ATM and ATR kinases in response to irradiation induced DSB in Arabidopsis