◀ Back to LCK
CBL — LCK
Pathways - manually collected, often from reviews:
-
KEGG T cell receptor signaling pathway:
CBL/CBLB/CBLC
→
LCK
(protein-protein, ubiquination)
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
TCR/CD3/MHC II/CD4/LCK/CBL/Ubiquitin complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247-CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247)
→
CBL (CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/CBL/Ubiquitin complex (CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-CD247-CBL)
→
CBL (CBL)
(modification, collaborate)
Rao et al., Proc Natl Acad Sci U S A 2002
Evidence: assay
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
CBL (CBL)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
CBL (CBL)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70/CBL/ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70-CBL)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD8+ T cells:
TCR/CD3/MHC I/CD8/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70)
→
TCR/CD3/MHC I/CD8/LCK/ZAP-70/CBL/ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-A-B2M-CD8A-CD8B-LCK-ZAP70-CBL)
(modification, collaborate)
Davanture et al., J Immunol 2005
Evidence: mutant phenotype, other species
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
CBL (CBL)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
SLAP-2 (SLA2)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
TCR/CD3/MHC II/CD4/LCK/ZAP-70/CBL/SLAP-2/Ubiquitin complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70-CBL-SLA2)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
NCI Pathway Database TCR signaling in naïve CD4+ T cells:
CBL (CBL)
→
TCR/CD3/MHC II/CD4/LCK/ZAP-70 complex (CD247-CD3E-CD3G-CD3D-HLA-DRA-HLA-DRB1-CD4-LCK-ZAP70)
(modification, collaborate)
Wang et al., J Biol Chem 2001, Loreto et al., Mol Cell Biol 2002
Evidence: assay, physical interaction
-
Reactome Reaction:
CBL
→
LCK
(reaction)
Rao et al., J Leukoc Biol 2002*, Wollberg et al., Biochem J 2003, Masson et al., Biochem J 2006*, Sun et al., Exp Cell Res 2007
-
Reactome Reaction:
CBL
→
LCK
(indirect_complex)
Rao et al., J Leukoc Biol 2002*, Wollberg et al., Biochem J 2003, Masson et al., Biochem J 2006*, Sun et al., Exp Cell Res 2007
-
WikiPathways T-Cell antigen Receptor (TCR) pathway during Staphylococcus aureus infection:
CBL
→
LCK
(mim-inhibition)
Protein-Protein interactions - manually collected from original source literature:
Studies that report less than 10 interactions are marked with *
-
IRef Biogrid Interaction:
LCK
—
CBL
(physical association, affinity chromatography technology)
Rao et al., Proc Natl Acad Sci U S A 2002
-
IRef Biogrid Interaction:
LCK
—
CBL
(direct interaction, pull down)
Tsygankov et al., J Biol Chem 1996*
-
IRef Biogrid Interaction:
LCK
—
CBL
(physical association, affinity chromatography technology)
Nervi et al., Gene 2005*
-
IRef Biogrid Interaction:
LCK
—
CBL
(direct interaction, pull down)
Reedquist et al., J Biol Chem 1996*
-
IRef Hprd Interaction:
LCK
—
CBL
(in vivo)
Hawash et al., J Biol Chem 2002*, Rao et al., Proc Natl Acad Sci U S A 2002, Husson et al., Oncogene 1997*
-
IRef Intact Interaction:
LCK
—
CBL
(physical association, coimmunoprecipitation)
Marcilla et al., J Biol Chem 1995*
-
IRef Intact Interaction:
LCK
—
CBL
(direct interaction, pull down)
Marcilla et al., J Biol Chem 1995*
-
IRef Intact Interaction:
LCK
—
CBL
(physical association, pull down)
Nervi et al., Gene 2005*
-
IRef Intact Interaction:
LCK
—
CBL
(physical association, coimmunoprecipitation)
Panchamoorthy et al., J Biol Chem 1996
-
IRef Ophid Interaction:
LCK
—
CBL
(aggregation, confirmational text mining)
Hawash et al., J Biol Chem 2002*
-
IRef Ophid Interaction:
LCK
—
CBL
(aggregation, interologs mapping)
Brown et al., Bioinformatics 2005
Text-mined interactions from Literome
van Leeuwen et al., Mol Cell Biol 1999
(Cell Transformation, Neoplastic) :
The requirement for Fyn,
Lck , and ZAP-70 is not
due to tyrosine phosphorylation of 70Z
Cbl , as mutation of all tyrosines in, or deletion of, the C-terminal region of 70Z Cbl ( amino acids 655 to 906 ) blocks 70Z Cbl tyrosine phosphorylation but enhances 70Z Cbl mediated NFAT activation
Rao et al., Proc Natl Acad Sci U S A 2002
:
Negative
regulation of
Lck by
Cbl ubiquitin ligase ... Coexpression in 293T cells demonstrated that Lck kinase activity and
Cbl ubiquitin ligase activity were
essential for
Lck ubiquitination and negative regulation of Lck dependent serum response element-luciferase reporter activity ... The Lck SH3 domain was pivotal for Cbl-Lck association and
Cbl mediated
Lck degradation, with a smaller role for interactions mediated by the Cbl tyrosine kinase binding domain ... Our results demonstrate a direct, ubiquitination dependent, negative regulatory
role of
Cbl for
Lck in T cells, independent of Cbl mediated regulation of ZAP-70
Yang et al., Virology 2005
(HIV Infections) :
Furthermore,
Lck is
required for Nef mediated
c-Cbl tyrosine phosphorylation