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E2F1 — MDM2

Pathways - manually collected, often from reviews:

• OpenBEL Selventa BEL large corpus: E2F1 → MDM2 (directlyIncreases, MDM2 Activity)
  Evidence: Mdm2 binds with E2F1:TFDP1 - this enhances the transcriptional activity of E2F1:TFDP1
• BioCarta p53 signaling pathway: MDM2 → RB/E2F-1 complex (E2F1-RB1) (modification, activates)
  
• BioCarta p53 signaling pathway: MDM2 → E2F-1 (E2F1) (modification, activates)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Biogrid Interaction: MDM2 — E2F1 (physical association, affinity chromatography technology) Zhang et al., Oncogene 2005*
• IRef Biogrid Interaction: MDM2 — E2F1 (direct interaction, enzymatic study) Stevens et al., FEBS J 2008*
• IRef Biogrid Interaction: MDM2 — E2F1 (physical association, affinity chromatography technology) Stevens et al., FEBS J 2008*
• IRef Hprd Interaction: Complex of MDM2-RB1-E2F1-RB1-MDM2-E2F1-E2F1-MDM2-RB1 (in vivo) Markham et al., EMBO Rep 2006

Text-mined interactions from Literome

Reinke et al., Cell Growth Differ 1999 : Because MDM2 also binds and activates the S phase-specific transcription factor E2F1 , we hypothesized that increased E2F1 activity causes the development of the BLGmdm2 phenotype
Yang et al., Clin Cancer Res 1999 (Neoplasms, Experimental) : Adenovirus mediated E2F-1 gene transfer inhibits MDM2 expression and efficiently induces apoptosis in MDM2 overexpressing tumor cells ... These results indicate that adenovirus mediated E2F-1 overexpression in MDM2 overexpressing tumor cells results in decreased MDM2 expression and widespread apoptosis
Wunderlich et al., Oncogene 2002 : Mdm2 inhibition of p53 induces E2F1 transactivation via p21 ... Using a set of cell lines with differing p53 and Rb status we determined that Mdm2 induction of E2F1 transactivation was p53 dependent, resulting from release of repression by p53 ... Consistent with Mdm2 activation of E2F1 via an inhibition of p53 transactivation we demonstrate a concomitant reduction in p21 protein levels with Mdm2 overexpression ... Taken together, these results suggest that Mdm2 activation of E2F1 occurs through the repression of p53 dependent transcription of p21, a p53-target gene and cyclin dependent kinase inhibitor
Zhang et al., Proc Natl Acad Sci U S A 2003 (Prostatic Neoplasms) : In PC3 cells, MDM2 inhibition resulted in elevated p21, Bax, and pRb levels and decreased ppRb and E2F1 levels
Wunderlich et al., Cell cycle (Georgetown, Tex.) 2004 : While examining the effect of Mdm2 overexpression on E2F1 transactivation we uncovered a novel MdmX function, the ability to inhibit E2F1 transactivation in a p53 and Mdm2 independent manner
Sdek et al., J Biol Chem 2004 : In addition, we show that overexpression of MDM2 reduces Rb-E2F complexes in vivo ... Moreover, the ectopic expression of the wild type MDM2, but not mutant MDM2 defective in Rb interaction, stimulates E2F transactivation activity and inhibits Rb growth suppression function
Zhang et al., Oncogene 2005 (Breast Neoplasms...) : The present study investigated the mechanisms responsible for the MDM2 mediated increase in E2F1 expression
Zhang et al., Ann N Y Acad Sci 2005 : This article summarizes the biochemical and molecular studies of the role of MDM2 in the regulation of p21 and E2F1 expression, stability and function, providing evidence for the utility of RNA silencing technologies, including antisense oligonucleotides and siRNAs
Huart et al., J Biol Chem 2009 : Although the treatment of cells with D4476 resulted in a partial p53 dependent growth arrest, the induction of p53 independent apoptosis by D4476 suggested a critical role for the MDM2 x CK1 complex in maintaining E2F-1 anti-apoptotic signaling