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TNF — WNK1

Text-mined interactions from Literome

Manna et al., Biochem Pharmacol 1999 : TNF induced NF-kappaB activation, p65 translocation, IkappaBalpha degradation, and NF-kappaB dependent reporter gene expression were inhibited in cells pretreated with beta-lapachone
Diaz-Meco et al., J Biol Chem 1999 : We show here that expression of Par-4 inhibits the TNFalpha induced nuclear translocation of p65 as well as the kappaB dependent promoter activity
Rohr et al., Nucleic Acids Res 1999 : However, in contrast with TNF-alpha , dopamine treatment did not affect NF-kappaB DNA binding activity nor the concentrations of p50, p65 and IkappaB-alpha proteins, which suggests a distinct NF-kappaB activation mechanism
Yoo et al., J Immunol 2000 : Although HSP induction did not affect total cellular expression of NF-kappa B, TNF-alpha induced increase in NF-kappa B-DNA binding activity and nuclear translocation of the p65 subunit of NF-kappa B were inhibited by prior HSP induction, suggesting that activation of NF-kappa B was blocked
Manna et al., J Immunol 2000 : Resveratrol also suppressed TNF induced phosphorylation and nuclear translocation of the p65 subunit of NF-kappaB, and NF-kappaB dependent reporter gene transcription
Marshall et al., Biochemistry 2001 : This inhibition was reversed by addition of the denitrosylating agent dithiothreitol to cellular extracts, whereas NO bioactivity did not affect the TNFalpha induced degradation of IkappaBalpha or the nuclear translocation of p65
Ariga et al., J Biol Chem 2002 : Moreover, DHMEQ inhibited the TNF-alpha induced nuclear accumulation of p65 , a component of NF-kappaB
Gutierrez et al., J Biol Chem 2002 (Crohn Disease...) : Upon stimulation with TNFalpha or lipopolysaccharide, both p50 and p65 subunits of NF-kappaB were bound to the Nod2 promoter
Umezawa et al., Mol Cells 2002 : Moreover, DHMEQ inhibited the TNF-alpha induced nuclear accumulation of p65 , a component of NF-kappaB
Ashikawa et al., J Immunol 2002 : When examined for the mechanism, we found that piceatannol inhibited TNF induced IkappaBalpha phosphorylation, p65 phosphorylation, p65 nuclear translocation, and IkappaBalpha kinase activation, but had no significant effect on IkappaBalpha degradation
Moustafa et al., J Invest Dermatol 2002 : Inhibition of tumor necrosis factor-alpha stimulated NFkappaB/p65 in human keratinocytes by alpha-melanocyte stimulating hormone and adrenocorticotropic hormone peptides
Okazaki et al., Biochem Biophys Res Commun 2003 : Moreover, TNF induced phosphorylation of p65 was severely impaired in S276A mutant, indicating that S276 is the major phosphorylation site of p65 and its phosphorylation is essential for p65 dependent cellular responses
Chen et al., Kidney Int 2003 : The present data indicate that TNF-alpha activation of PKCzeta/iota, p42/44 MAPK, c-Jun/AP-1, and p65/NF-kappaB are involved in TNF-alpha stimulated MC fractalkine expression, with the soluble fractalkine mediating in part the TNF-alpha induced monocyte transmigration in vitro
Uchiba et al., Crit Care Med 2003 : Tumor necrosis factor-alpha induced increase in DNA binding of p65 was inhibited by gabexate mesilate through inhibition of the nuclear translocation of p65
Hanson et al., J Biol Chem 2003 : This suppression of NF kappa B is manifested by an inhibition of TNF induced inhibitor of NF kappa B ( IKK ) activity and NF kappa B DNA binding potential but not by blocking TNF induced nuclear accumulation of NF kappa B/p65
Joo et al., Biochem Biophys Res Commun 2003 (Hepatoblastoma...) : Electrophoretic mobility shift assays showed that TNFalpha induced p65 binding to a potential NF-kappaB binding site at -460/-451
Monteleone et al., J Biol Chem 2004 (Crohn Disease) : Pretreatment of normal human intestinal lamina propria mononuclear cells ( LPMC ) with transforming growth factor-beta1 ( TGF-beta1 ) resulted in a marked suppression of TNF-alpha induced NF-kappaB p65 accumulation in the nucleus, NF-kappaB binding DNA activity, and NF-kappaB dependent gene activation
Giri et al., Apoptosis 1999 : TNF induced NF-kappa B activation, I kappa B alpha degradation, and nuclear translocation of p65 were all found to be highly suppressed in the U-937-VR cells
Takada et al., J Biol Chem 2004 : PTD-p65-P1 had no effect on TNF induced inhibitory subunit of NF-kappaB ( IkappaBalpha ) phosphorylation, IkappaBalpha degradation, or IkappaBalpha kinase activation, but it blocked TNF induced p65 phosphorylation and nuclear translocation
Miao et al., J Biol Chem 2004 (Diabetes Mellitus...) : Overexpression of histone deacetylase (HDAC) isoforms inhibited p65 mediated TNF-alpha transcription
Mitsuyama et al., J Bone Miner Res 2004 (Calcium Signaling) : EMSA revealed that this site binds with NF-kappaB containing p65 that was activated by Ion/PMA and TNF-alpha and that CnI inhibited only Ion/PMA dependent NF-kappaB activation
Hu et al., Carcinogenesis 2004 (Cell Transformation, Neoplastic) : Phosphorylation of p65 at S536 appears to play a role in TNF-alpha induced p65 DNA binding and recruitment of p300 to the p65 complex as well as in release of p65 bound to HDAC1 and 3
Kwon et al., Mol Cell Biol 2004 : SIMPL interacts with nuclear p65 in a TNF-alpha dependent manner to promote endogenous NF-kappaB dependent gene expression
Wadgaonkar et al., J Cell Biochem 2005 : Furthermore, our data revealed that inhibition of MLCK attenuated the TNFalpha induced IkappaB phosphorylation, translocation of p65 , NFkappaB-DNA binding, and NFkappaB transcriptional activity
Uchiba et al., Thromb Haemost 2004 : AT inhibited the TNF-alpha induced interaction of NF-kappaB p65 with p300, a homologue of cAMP-responsive element binding protein ( CREB) binding protein (CBP )
Hall et al., Am J Physiol Heart Circ Physiol 2005 : Overexpression of dominant negative p65 reduced TNF-alpha production 3.5-fold, whereas overexpression of dominant negative IKK-beta reduced LPS induced TNF-alpha production 2-fold and p65 phosphorylation 2-fold
Chen et al., Invest Ophthalmol Vis Sci 2005 : IL-1beta, TNFalpha , and VEGF ( 165 ) induced nuclear translocation and binding of p65 and p50 NF-kappaB isoforms to the VCAM-1 promoter
Corbetta et al., Endocr Relat Cancer 2005 (Parathyroid Neoplasms) : Tumor necrosis factor-alpha (TNFalpha) caused a significant increase in phosphorylated p65 levels ( 183 +/- 13.8 % of basal ) while calcium sensing receptor (CaR) agonists exerted a significant inhibition ( 19.2 +/- 3.3 % of basal )
Takada et al., J Biol Chem 2006 (Neoplasm Invasiveness) : We also found that SAHA had no effect on direct binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation
Ye et al., Am J Physiol Gastrointest Liver Physiol 2006 : Additionally, siRNA silencing of NF-kappaB p65 also prevented the TNF-alpha increase in MLCK promoter activity
Takada et al., J Immunol 2006 : AKBA did not directly affect the binding of NF-kappaB to the DNA but inhibited sequentially the TNF induced activation of IkappaBalpha kinase (IKK), IkappaBalpha phosphorylation, IkappaBalpha ubiquitination, IkappaBalpha degradation, p65 phosphorylation, and p65 nuclear translocation
Issa et al., Am J Physiol Lung Cell Mol Physiol 2006 (MAP Kinase Signaling System) : AS-602868 but not PD-98059 or SP-600125 inhibited p65 DNA binding induced by IL-1beta and TNF-alpha
Sandur et al., J Biol Chem 2006 (Inflammation) : The suppression of NF-kappaB activation correlated with sequential inhibition of the tumor necrosis factor (TNF) induced activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 phosphorylation, p65 nuclear translocation, and the NF-kappaB dependent reporter gene expression activated by TNF, TNFR1, TRAF2, NIK, IKK-beta, and the p65 subunit of NF-kappaB
Fitau et al., J Biol Chem 2006 : Mechanistically, our data indicated that, in response to TNFalpha , NFkappaB/p65 phosphorylation and translocation as well as IkappaBalpha phosphorylation and degradation were unchanged, suggesting that Lnk does not modulate NFkappaB activity
Jun et al., Inflamm Bowel Dis 2006 (Body Weight...) : Pretreatment of human epithelial HT-29 cells with gliotoxin significantly blocked the I-kappaB degradation and NF-kappaB p65 nuclear translocation induced by tumor necrosis factor-alpha or IL-1beta ; these were parallel with the inhibition of IL-8 secretion and intercellular adhesion molecule-1 expression in the same cells
Morello et al., J Immunol 2006 : IL-1beta and TNF-alpha rapidly activated p50 and p65 , but not C-Rel, RelB, or p52, and both IL-1beta- and TNF-alpha stimulated A2AR expression was inhibited by the IkappaB kinase 2 inhibitor AS602868 in a concentration dependent manner
Sethi et al., Blood 2007 (Neoplasm Invasiveness...) : Celastrol was found to inhibit the TNF induced activation of IkappaBalpha kinase, IkappaBalpha phosphorylation, IkappaBalpha degradation, p65 nuclear translocation and phosphorylation, and NF-kappaB mediated reporter gene expression
Choo et al., Mol Cancer Ther 2006 : To clarify the molecular mechanism of TRAIL induced apoptosis, we focused on transforming growth factor-beta activated kinase 1 ( TAK1 ) mitogen activated protein kinase ( MAPK ) kinase kinase, a key regulator of the TNF-alpha induced activation of p65/RelA and c-Jun NH2-terminal kinase/p38 MAPKs
D'Alimonte et al., J Immunol 2007 : Moreover, guanosine abrogated IFN-gamma induced phosphorylation on Ser ( 727 ) and translocation of STAT-1alpha to the nucleus as well as TNF-alpha-/Abeta induced IkappaBalpha and NF-kappaB p65/RelA subunit phosphorylation, thus inhibiting NF-kappaB induced nuclear translocation
Ahn et al., Blood 2007 (Neoplasm Invasiveness) : Further studies showed that salinosporamide A inhibited TNF induced inhibitory subunit of NF-kappaB alpha ( IkappaBalpha ) degradation, nuclear translocation of p65 , and NF-kappaB dependent reporter gene expression but had no effect on IkappaBalpha kinase activation, IkappaBalpha phosphorylation, or IkappaBalpha ubiquitination
Sethi et al., J Immunol 2007 : Further investigation revealed that TNF induced I kappaB alpha kinase activation, I kappaB alpha phosphorylation, I kappaB alpha degradation, and p65 nuclear translocation were all suppressed in MKK4-KO cells
Wang et al., J Biol Chem 2007 (Thrombosis) : By electrophoretic mobility shift assay analyses, TNF-alpha and lipopolysaccharide induce strong p65/p50 and p65/c-Rel heterodimer binding to both NF-kappaB and TF-kappaB probes
Lee et al., Biochem Pharmacol 2007 (Colitis...) : Interestingly, the latter is mediated by HO-1, which presumably blocks the TNF-alpha induced nuclear translocation of NF-kappaB p65 without affecting I-kappaBalpha degradation
Kitano et al., Invest Ophthalmol Vis Sci 2007 (Conjunctivitis, Allergic...) : Emodin inhibited TNF-alpha induced NF-kappaB p65 and JNK activation but did not affect transforming growth factor beta1 induced Smad2/3 signaling
Yoon et al., Cell Death Differ 2008 : Furthermore, TNF induced p65/RelA phosphorylation as well as transcriptional activity of nuclear factor-kappaB (NF-kappaB) was significantly downregulated in T6 ( -/- ) 3T3 cells
László et al., Photochem Photobiol 2008 : We also demonstrate that UV only induced phosphorylation of p65 ( S276 ), while tumor necrosis factor-alpha induced phosphorylation at both Ser276 and 536 sites of p65
Yomogida et al., Int J Mol Med 2008 : Furthermore, glucosamine inhibited the TNF-alpha induced phosphorylation of p38MAPK and NF-kappaB p65 , and the nuclear translocation of NF-kappaB in the cells
Tsoporis et al., J Biol Chem 2008 (Inflammation) : Electrophoretic mobility shift showed that tumor necrosis factor-alpha induced p65 binding to a potential NF-kappaB binding site at -460/-451
Kustermans et al., Biochem Pharmacol 2008 : Interestingly, actin disruption primed p65 phosphorylation induced by TNFalpha and LPS, on Ser ( 276 ) and Ser ( 536 ), respectively, which suggested actin cytoskeleton could also modulate p65 transactivating activity
Choi et al., Cancer Res 2009 (Cell Transformation, Viral) : We also showed that EGCG prevents TNFalpha induced p65 translocation to the nucleus, confirming that hyperacetylation is critical for NF-kappaB translocation as well as activity
Bao et al., Am J Respir Crit Care Med 2009 (Asthma) : In normal human bronchial epithelial cells, andrographolide blocked tumor necrosis factor-alpha induced phosphorylation of inhibitory kappaB kinase-beta, and downstream inhibitory kappaB alpha degradation, p65 subunit of NF-kappaB phosphorylation, and p65 nuclear translocation and DNA binding activity
Du et al., Cancer Res 2009 (Neoplasms) : Furthermore, SW480 cells stably transformed with wild-type adenomatous polyposis coli showed decreased beta-catenin protein and increased TNFalpha induced p65 NF-kappaB binding as well as iNOS and Traf1 expression
Yu et al., Cell Signal 2009 : We demonstrated that p300 physically associated with p65 rather than Smad4 in the presence of both TNF-alpha and TGF-beta
Seidel et al., Am J Physiol Lung Cell Mol Physiol 2009 (Chronic Disease...) : DMF inhibited TNFalpha induced NF-kappaB p65 phosphorylation, NF-kappaB nuclear entry, and NF-kappaB-DNA complex formation, whereas PDGF-BB appeared not to activate NF-kappaB within 60 min
Hwang et al., Am J Chin Med 2009 : Furthermore, EGE significantly inhibited TNF-alpha induced intracellular reactive oxygen species ( ROS ) production and p65 NF-kappaB activation by preventing IkappaB-alpha phosphorylation
Ji et al., Anticancer Drugs 2009 (Lung Neoplasms) : TNF-alpha treatment induced an elevated activated NFkappaB/p65 , concomitant with induced p21 levels, which resulted in increased sensitivity to gefitinib in PC-9-ZD cells
Gossye et al., Arthritis Rheum 2009 (Arthritis, Rheumatoid) : In addition, CpdA attenuated the TNFalpha induced nuclear translocation and DNA binding of p65 in RA FLS, via the attenuation of IKK phosphorylation and subsequent IkappaBalpha degradation
Tseng et al., J Cell Physiol 2010 : Stimulation of myoblasts with TNF-alpha activated IkappaB kinase alpha/beta ( IKKalpha/beta ), IkappaBalpha phosphorylation, p65 phosphorylation, and kappaB-luciferase activity
Hwang et al., Phytother Res 2010 : A further analysis indicated that EGJ attenuated TNF-alpha induced nuclear p65 nuclear factor-kappa B (NF-kappaB) translocation, suggesting that EGJ primarily affects the TNF-alpha induced NF-kappaB signaling pathway
Yang et al., Atherosclerosis 2010 : N-acetylcysteine, SP600125, SB203580 and MPA had no effect on either TNF-alpha induced IkappaBalpha degradation or p65 nuclear translocation, but attenuated p65 Ser276 phosphorylation
Diel et al., J Virol 2010 (Ecthyma, Contagious) : Further, ORFV024 expression decreased TNF-alpha induced phosphorylation and nuclear translocation of NF-kappaB-p65 , phosphorylation, and degradation of IkappaBalpha, and phosphorylation of IkappaB kinase (IKK) subunits IKKalpha and IKKbeta, indicating that ORFV024 functions by inhibiting activation of IKKs, the bottleneck for most NF-kappaB activating stimuli
Liao et al., Toxicological sciences : an official journal of the Society of Toxicology 2010 : ECs treated with GSH and H ( 2 ) O ( 2 ) show increased sulfhydryl modifications of the p65 subunit of nuclear factor kappa-light-chain-enhancer of activated B cells ( NF-kappaB ), which are responsible for NF-kappaB inactivation, and also a block in TNF-alpha induced p65 nuclear translocation and inter-cellular adhesion molecule-1 ( ICAM-1 ) expression ... In our current study, we find that cinnamaldehyde induces p65 glutathionylation and inhibits TNF-alpha induced p65 nuclear translocation and ICAM-1 expression within 12 h of treatment
Li et al., Molecular cancer 2010 : Addition of MA inhibited TNFalpha induced IkappaBalpha degradation, p65 phosphorylation, and nuclear translocation
Lee et al., Eur J Pharmacol 2010 : In HCT116 cells, CAPE interfered with TNF-alpha dependent IkappaBalpha degradation and subsequent nuclear accumulation of p65 , which occurred by direct inhibition of inhibitory protein kappaB kinase (IKK)
Lee et al., Am J Respir Cell Mol Biol 2011 : a-MSH suppressed NF-?B/p65 phosphorylation induced by TNF-a as well as IkB-a degradation in a dose dependent manner, as assessed by Western blotting
Sasaki et al., J Biol Chem 2011 : The LTß priming process was not due to an increase in TNF mediated nuclear translocation of p65 , p65 DNA binding, or NF-?B transactivational activity
Li et al., J Biol Chem 2011 (Diabetic Retinopathy...) : Conversely, overexpression of spliced XBP1 attenuated TNF-a induced phosphorylation of IKK, I?Ba, and NF-?B p65 , accompanied by decreased NF-?B activity and reduced adhesion molecule expression
Watari et al., Int J Cancer 2012 : We found that the TNF-a induced phosphorylation of nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha ( I?Ba ) and nuclear translocation of p65 were impaired by AMF-26 in both endothelial cells and cancer cells
Di Bartolo et al., Lipids in health and disease 2011 (Chronic Disease...) : When isolated rabbit HDL was pre incubated with human coronary artery endothelial cells ( HCAECs ), prior to stimulation with TNF-a, it was found that HDL from ETC-642 treated rabbits were more effective at inhibiting the TNF-a induced increase in ICAM-1, VCAM-1 and p65 than HDL isolated from saline treated rabbits ( p < 0.05 )
Lee et al., Phytother Res 2012 : The results were as follows : ( 1 ) resveratrol inhibited the expression of MUC5AC gene induced by EGF or PMA or TNF-a from NCI-H292 cells ; ( 2 ) resveratrol also inhibited the production of MUC5AC mucin protein induced by the same inducers from NCI-H292 cells ; ( 3 ) resveratrol inhibited the activation of NF-?B p65 by TNF-a or PMA in NCI-H292 cells ; ( 4 ) resveratrol significantly decreased ATP induced mucin secretion from cultured RTSE cells
Kim et al., Phytother Res 2012 : The effect of silibinin on TNF-a induced activation of NF-?B p65 was also examined
Ma et al., J Pharmacol Sci 2012 : However, this compound did not interfere with tumor necrosis factor-a (TNF-a) induced inhibitor of ?Ba ( I?Ba ) degradation, p65 nuclear translocation, and DNA binding activity of NF-?B
Peng et al., Am J Physiol Cell Physiol 2012 : In addition, we demonstrated that, by affecting the nuclear translocation of p65, ID1 is essential in regulating TNF-a induced p65 recruitment to its downstream target, the cellular inhibitor of apoptosis protein 2 ( cIAP2 ) promoter
Matsui et al., Intensive Care Med 2012 : The production of tumor necrosis factor-alpha ( TNF-a ), interleukin-10 (IL-10), and NO and the activation of NF-?B p65 were reduced by hypothermia, but augmented by hyperthermia at 3-6, 24-48, 48, and 0.5 h, post-treatment initiation, respectively
Wu et al., Food Funct 2012 : The flavonoid extract attenuated TNF-a induced I?B phosphorylation as well as NF-?B, p65 and p50 translocation from cytosol to nucleus, through inhibition on TNF-a- and H ( 2 ) O ( 2 ) -induced intracellular reactive oxygen species ( ROS ) production
Nguyen et al., Int J Oncol 2012 : Interestingly, reduction of MMS22L by siRNAs in cancer cells inhibited the TNF-a dependent activation of RelA/p65 in the NFKB pathway and expression of its downstream anti-apoptotic molecules such as Bcl-XL and TRAF1
Kwon et al., Atherosclerosis 2012 (Neointima) : Sulforaphane inhibited the mRNA and protein expression of VCAM-1 induced by tumor necrosis factor (TNF)-a in VSMCs. Treatment of VSMCs with sulforaphane blocked TNF-a induced I?Ba degradation and NF-?B p65 and GATA6 expression
Fujita et al., J Biol Chem 2012 (Intervertebral Disc Degeneration) : Prolyl Hydroxylase 3 (PHD3) Modulates Catabolic Effects of Tumor Necrosis Factor-a (TNF-a) on Cells of the Nucleus Pulposus through Co-activation of Nuclear Factor ?B ( NF-?B ) /p65 Signaling
Park et al., Br J Pharmacol 2013 (Arthritis, Rheumatoid...) : Moreover, LPS induced increase of cytokine production ( TNF-a , IL-1ß ) was inhibited by cilostazol, an effect which was accompanied by suppression of I?Ba degradation, and NF-?B p65 nuclear translocation
Yang et al., Cell Physiol Biochem 2012 : The frther sudy idicated that PDTC ( NF-?B inhibitor ) pretreatment attenuated TNF-a induced CD40 expression, and SIRT1 siRNA significantly augmented TNF-a induced acetylated-NF-?B p65 ( Lys310 ) expression
Wang et al., World journal of surgical oncology 2013 (Carcinoma, Non-Small-Cell Lung...) : In the study, we designed to investigate the effect of TNF-a on the activation and expression of nuclear factor kappa B ( NF-?B ) /p65/SLUG/PUMA/Bcl-2 levels in human lung cancer A549 cell line, and in conditions of TNF-a induced apoptosis
Shi et al., PloS one 2013 (Prostatic Neoplasms) : Western blot assay indicated that PMS1077 suppressed the TNF-a induced inhibitor of ?B-a ( I?B-a ) phosphorylation, I?B-a degradation, and p65 phosphorylation
Naumann et al., EMBO J 1994 : In HeLa cells as well as in B cells, TNF-alpha rapidly induced nuclear translocation primarily of p50-p65 , but not of c-rel
Jahnke et al., FEBS Lett 1994 (Melanoma) : Synergistic activation of intercellular adhesion molecule 1 ( ICAM-1 ) by TNF-alpha and IFN-gamma is mediated by p65/p50 and p65/c-Rel and interferon-responsive factor Stat1 alpha ( p91 ) that can be activated by both IFN-gamma and IFN-alpha
Jahnke et al., Immunobiology 1995 (Melanoma) : Using electrophoretic mobility shift assays and specific antibodies we observed that the NF-kappa B like sequence at -187 bound both p65/p50 and p65/c-Rel in the presence of TNF-alpha , while the interferon responsive region at -75 bound Stat1 alpha ( p91 )
Drew et al., J Interferon Cytokine Res 1995 : We demonstrated here that TNF-alpha induced binding of NF kappa B p50 and p65 to the NF kappa B-like element of the MHC class I promoter termed region I and IFN-gamma induced binding of IRF-1 to the adjacent interferon consensus sequence ( ICS )
Kikumori et al., Endocrinology 1998 : The treatment with the antioxidants 20 mM N-acetyl-L-cysteine (NAC) and 10 microM pyrrolidine dithiocarbamate ( PDTC ) inhibited the TNF-alpha dependent activation of p65-p50 heterodimer but not the p50-p50 homodimer, indicating that generation of oxidants is required for the activation of the heterodimer NF-kappaB
Manna et al., J Biol Chem 1998 : Overexpression of manganese superoxide dismutase ( Mn-SOD ) in human breast cancer MCF-7 cells completely abolished TNF mediated NF-kappaB activation, IkappaB alpha degradation, p65 nuclear translocation, and NF-kappaB dependent reporter gene expression
Kurokouchi et al., J Bone Miner Res 1998 (Bone Neoplasms...) : These results suggest that the rapid activation of NF-kappaB by TNF-alpha is mainly due to the nuclear translocation of NF-kappaB pre existing in cytosol, and that the subsequent increase in the expression of p50 and p65 may result in the persistent activation of NF-kappaB during TNF-alpha stimulation
Yang et al., Am J Physiol 1998 (Alcoholism) : TNF-alpha dependent increases in hepatic nuclear factor-kappaB (NF-kappaB) p50 and p65 expression and DNA binding activity are prevented, whereas IL-6 dependent inductions of hepatic Stat-3 phosphorylation and DNA binding activity occur normally