Barsacchi et al., Mol Pharmacol 2003
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Activation of endothelial nitric-oxide synthase by tumor necrosis factor-alpha : a novel pathway involving sequential activation of neutral sphingomyelinase , phosphatidylinositol-3 ' kinase, and Akt ... Akt and eNOS phosphorylation, as well as eNOS activation , were blocked by inhibitors of both phosphatidylinositol-3 ' kinase and neutral sphingomyelinase Mogami et al., FEBS Lett 2005
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N-SMase induced translocation of endothelial NOS (eNOS) from plasma membrane caveolae to intracellular region, eNOS phosphorylation on serine 1179, and an increase of ceramide level in endothelial cells ... We propose the involvement of N-SMase and ceramide in Ca ( 2+ ) -independent eNOS activation and NO production in endothelial cells in situ, linking to endothelium dependent vasorelaxation