◀ Back to SMAD5

SMAD1 — SMAD5

Pathways - manually collected, often from reviews:

• KEGG TGF-beta signaling pathway: Complex of SMAD1-SMAD4-SMAD5-SMAD9 → ID1/ID2/ID3/ID4 (gene expression, expression)
• KEGG TGF-beta signaling pathway: MAPK1/MAPK3 → Complex of SMAD1-SMAD4-SMAD5-SMAD9 (protein-protein, inhibition)
  
• NCI Pathway Database ALK2 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Visser et al., Mol Endocrinol 2001, Clarke et al., Mol Endocrinol 2001
  Evidence: mutant phenotype, assay
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Scharpfenecker et al., J Cell Sci 2007, Macías-Silva et al., J Biol Chem 1998, Chen et al., J Biol Chem 1999
  Evidence: assay
• NCI Pathway Database ALK2 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Macías-Silva et al., J Biol Chem 1998, Chen et al., J Biol Chem 1999
  Evidence: assay, physical interaction
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Oh et al., Proc Natl Acad Sci U S A 2000, Goumans et al., EMBO J 2002, Goumans et al., Mol Cell 2003
  Evidence: mutant phenotype, assay
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Oh et al., Proc Natl Acad Sci U S A 2000, Goumans et al., EMBO J 2002, Goumans et al., Mol Cell 2003, Lebrin et al., EMBO J 2004, Blanco et al., J Cell Physiol 2005, Lee et al., FASEB J 2009
  Evidence: mutant phenotype, assay
• NCI Pathway Database ALK2 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD4/SMAD1-5-8 (dimer) complex (SMAD4-SMAD1_SMAD9_SMAD5) (modification, collaborate)
  Visser et al., Mol Endocrinol 2001, Clarke et al., Mol Endocrinol 2001, Macías-Silva et al., J Biol Chem 1998
  Evidence: assay
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD4/SMAD1-5-8 (dimer) complex (SMAD4-SMAD1_SMAD9_SMAD5) (modification, collaborate)
  Oh et al., Proc Natl Acad Sci U S A 2000, Goumans et al., EMBO J 2002, Goumans et al., Mol Cell 2003
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD4/SMAD1-5-8 (dimer) complex (SMAD4-SMAD1_SMAD9_SMAD5) (modification, collaborate)
  Scharpfenecker et al., J Cell Sci 2007, Chen et al., J Biol Chem 1999
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  David et al., Blood 2007, Scharpfenecker et al., J Cell Sci 2007
  Evidence: mutant phenotype, assay
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Oh et al., Proc Natl Acad Sci U S A 2000, Goumans et al., EMBO J 2002, Goumans et al., Mol Cell 2003, Lebrin et al., EMBO J 2004, Blanco et al., J Cell Physiol 2005, Valdimarsdottir et al., BMC cell biology 2006, Santibanez et al., Cardiovasc Res 2008
  Evidence: mutant phenotype, assay
• NCI Pathway Database ALK1 signaling events: SMAD1-5-8-active (SMAD1/SMAD9/SMAD5) → SMAD1-5-8 (SMAD1/SMAD9/SMAD5) (modification, collaborate)
  Lee et al., FASEB J 2009, Macías-Silva et al., J Biol Chem 1998
  Evidence: mutant phenotype
• WikiPathways ESC Pluripotency Pathways: Complex of SMAD5-SMAD1-SMAD9-SMAD4 → Complex of SMAD1-SMAD5-SMAD9-SMAD4 (activation)
  
• WikiPathways ESC Pluripotency Pathways: Complex of SMAD5-SMAD1-SMAD9-SMAD4 → MAPK7/MAPK1/MAPK6/MAPK4/MAPK12 (inhibition)
  
• WikiPathways ESC Pluripotency Pathways: SMAD1/SMAD5/SMAD9 → Complex of SMAD5-SMAD1-SMAD9-SMAD4 (activation)
  
• WikiPathways ESC Pluripotency Pathways: SMAD5/SMAD1/SMAD9 → SMAD1/SMAD5/SMAD9 (activation)
• WikiPathways BMP Signaling Pathway in Eyelid Development: BMP4 → Complex of SMAD1-SMAD5 (activation)
• WikiPathways BMP Signaling Pathway in Eyelid Development: Complex of SMAD1-SMAD5 → SMAD4 (activation)

Protein-Protein interactions - manually collected from original source literature:

Studies that report less than 10 interactions are marked with *

• IRef Bind Interaction: SMAD1 — SMAD5 Colland et al., Genome Res 2004
• IRef Bind_translation Interaction: SMAD1 — SMAD5 (two hybrid) Colland et al., Genome Res 2004
• IRef Biogrid Interaction: SMAD5 — SMAD1 (direct interaction, two hybrid) Colland et al., Genome Res 2004
• IRef Dip Interaction: Complex of SMAD5-DROSHA-DDX5-SMAD1-DDX5-SMAD1-DROSHA-SMAD5 (anti bait coimmunoprecipitation) Davis et al., Nature 2008*
• Gene Ontology Complexes transcription factor complex: transcription factor complex complex (ATF7IP-GSC-GCM1-MAFB-NKX2-1-NKX2-5-RARG-KLF4-FOXF1-FOXF2-FOXE3-LDB1-ZFHX3-GATA6-SNAI3-FOXH1-KAT5-AHR-EYA3-NFATC2-CRX-MED27-HES6-SKI-XRCC6-ARNTL-SUB1-JUN-SOX17-SCX-DMBX1-TCF4-TCF7-PDLIM1-TFEB-LBX1-TRRAP-NAA16-EPAS1-MGA-PTF1A-HOXD12-MEF2B-TFDP3-TFDP1-HDAC2-YY1-SMAD9-CLOCK-ONECUT3-SMAD5-SMAD6-SMAD7-SMAD1-SMAD2-PRKDC-RCOR2-NHLH2-REL-TBX5-ARNTL2-BSX-HOXA10-HOXB13-MED23-PUS1-TAL1-RBL1-RBL2-MINA-HMGA1-BARX2-LEF1-EP300-PMF1-ARID5A-WWTR1-LMO2-LMO4-TP73-ABT1-CDK2-DKFZp686M216-CREG1-CTNNB1-SOX9-HAND2-SOX2-TEAD2-MYOG-TEAD4-CEBPA-GFI1B-MYOD1-ALX1-NPAS4-TFAP2D-FIGLA-ALX4-ETS1-PROP1-ASCL3-HCLS1-MSX1-MSX2-SRA1-AJUBA-MTA2-SIN3A-POU3F2-POU3F1-BEX1-MLXIPL-NR2E3-PBX2-ANKRD1-E2F6-E2F5-E2F4-E2F3-E2F2-E2F1-E2F8-CREBBP-ATF5-ATF4-POU2F3) Kaspar et al., J Biol Chem 1999, Wang et al., J Biol Chem 1999, Nagpal et al., J Biol Chem 1999, Blixt et al., Genes Dev 2000, Carlberg et al., Mol Cell Biol Res Commun 2000, Bae et al., Development 2000, Cairo et al., Hum Mol Genet 2001, Tutter et al., Genes Dev 2001, Willis et al., J Biol Chem 2002, Bayne et al., Mol Hum Reprod 2004, Schubert et al., J Biol Chem 2004, Han et al., J Mol Biol 2005, Rodriguez et al., EMBO J 2005, Han et al., Nucleic Acids Res 2005, Zhang et al., Mol Cell Biol 2007, Wong et al., Cell 2009, Stevens et al., Immunology 2009, Stefanovic et al., J Cell Biol 2009, Skokowa et al., Nat Med 2012, Ge et al., Cell 1994, Durocher et al., EMBO J 1997, Hogenesch et al., Proc Natl Acad Sci U S A 1998, Hellqvist et al., J Biol Chem 1998, Ryu et al., Nature 1999
• IRef Hprd Interaction: SMAD5 — SMAD1 (two hybrid) Colland et al., Genome Res 2004
• IRef Intact Interaction: SMAD5 — SMAD1 (physical association, two hybrid) Colland et al., Genome Res 2004
• IRef Ophid Interaction: SMAD5 — SMAD1 (aggregation, interologs mapping) Brown et al., Bioinformatics 2005

Text-mined interactions from Literome

Harada et al., J Biol Chem 2003 : HIPK2 efficiently inhibited Smad1/4 induced transcription from the Smad site containing promoter
Fuller et al., Ann Neurol 2007 (Demyelinating Diseases...) : Cultured mature astrocytes respond directly to BMPs with Smad1 translocation to the nucleus, increased phosphorylated Smad1/5/8 , and increases in glial fibrillary acidic protein and CSPG expression
Matsubara et al., J Biol Chem 2008 : Furthermore, overexpression of Smad1 and Smad4 up-regulated Osterix expression, and an inhibitory Smad , Smad6, markedly suppressed BMP2 induced Osterix expression in the Runx2-deficient cells
Wang et al., J Biol Chem 2011 : Thus, we conclude that CHIP inhibits the signaling activities of Smad1/5 by recruiting Smad1/5 from the functional R-/Co-Smad complex and further promoting the ubiquitination/degradation of Smad1/5 in a chaperone independent manner
Lv et al., Nan Fang Yi Ke Da Xue Xue Bao 2013 : Transfection of the BMSCs with Smad1 siRNA decreased the basal level of Smad1/5/8 protein expression, and also inhibited Sr-induced up-regulation of p-Smad1/5/8 and Runx2 expressions as well as Sr-induced enhancement of ALP activity and formation of mineralized nodules
Ellman et al., Gene 2013 : The synergism results from LfcinB mediated activation of Sp1 and SMAD signaling pathways by ( i ) phosphorylation of SMAD 1/5/8 ; ( ii ) downregulation of SMAD inhibitory factors [ i.e., noggin and SMAD6 ( inhibitory SMAD ) ] ; and ( iii ) upregulation of SMAD4 ( universal co-SMAD ) ... The synergism results from LfcinB mediated activation of Sp1 and SMAD signaling pathways by ( i ) phosphorylation of SMAD 1/5/8 ; ( ii ) downregulation of SMAD inhibitory factors [ i.e., noggin and SMAD6 ( inhibitory SMAD ) ] ; and ( iii ) upregulation of SMAD4 ( universal co-SMAD )