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EPHB2 — RAF1
Text-mined interactions from Literome
Ozaki et al., J Biol Chem 1999
:
Upstream of
ERK , Shc was shown to be activated, but its downstream
Raf1 and ERK were
inhibited
Huang et al., Oncogene 1999
:
Taxol induced apoptosis was associated with phosphorylation of both
c-Raf-1 and Bcl-2 and
activation of
ERK and JNK MAP kinases
English et al., J Biol Chem 1999
(Cell Transformation, Neoplastic) :
We found that
Raf-1 does
contribute to Ras activation of
ERK5 but in a manner that does not correlate with Raf-1 catalytic activity
DeFea et al., J Cell Biol 2000
:
The complex, which contains internalized receptor, beta-arrestin,
raf-1 , and activated ERK, is
required for
ERK1/2 activation
Piiper et al., Mol Pharmacol 2000
:
PTx also inhibited
ERK1/2 activation in response to the PKC activator 12-O-tetradecanoylphorbol-13-acetate ( TPA ) and epidermal growth factor (EGF) as well as
activation of
c-Raf-1 by EGF and CCK
Florio et al., J Physiol Paris 2000
(MAP Kinase Signaling System) :
We show that the activation of
ERK via SSTR1 is mediated by the betagamma subunit of a pertussis toxin-sensitive G-protein and
requires both the small G protein Ras and the serine/threonine kinase
Raf-1
Luttrell et al., Proc Natl Acad Sci U S A 2001
:
The phosphorylation of
ERK2 in beta-arrestin complexes was markedly
enhanced by coexpression of
cRaf-1 , and this effect is blocked by expression of a catalytically inactive dominant inhibitory mutant of MEK1
Reusch et al., J Biol Chem 2001
:
Pertussis toxin,
N17-Ras/Raf , and PD98059
prevented both the serum- and thrombin induced second phase
ERK phosphorylation and SM-MHC promoter activation
Kampfer et al., J Biol Chem 2001
:
Expression of kinase-defective, dominant negative ( DN ) mutants of nPKC-epsilon or aPKC-zeta inhibit
ERK activation by constitutively active
Raf-1
Fernandez-Patron et al., FASEB J 2001
(MAP Kinase Signaling System) :
Stimulation of
Erk activity was
due to activation of Ras,
Raf-1 , and MEK ( MAPK kinase )
Tohgo et al., J Biol Chem 2002
(MAP Kinase Signaling System) :
beta-Arrestin overexpression resulted in formation of a cytoplasmic pool of beta-arrestin bound phospho-ERK, decreased nuclear translocation of phospho-ERK1/2, and inhibition of Elk1-driven luciferase transcription even when
ERK1/2 was
activated by overexpression of
cRaf-1 in the absence of AT1aR stimulation
Chan et al., Mol Carcinog 2002
(Cell Transformation, Neoplastic...) :
Expression of G361S
Raf-1 in Rat2 fibroblasts
activated MEK and
ERK
Correa-Meyer et al., Am J Physiol Lung Cell Mol Physiol 2002
(MAP Kinase Signaling System) :
The inhibition of Grb2-SOS interaction with an SH3 binding sequence peptide, Ras with a farnesyl transferase inhibitor, and
Raf-1 with forskolin did not
affect the stretch induced
ERK1/2 phosphorylation
Dhillon et al., Mol Cell Biol 2002
:
Using RafS259 mutants we also demonstrate that
Raf-1 is the sole
target for PKA inhibition of
ERK and ERK induced gene expression, and that Raf-1 inhibition is mediated mainly through serine 259 phosphorylation
Nadal-Wollbold et al., FEBS Lett 2002
:
Platelet
ERK2 activation by thrombin is
dependent on calcium and conventional protein kinases C but not
Raf-1 or B-Raf
Norum et al., J Biol Chem 2003
(MAP Kinase Signaling System) :
ERK1/2 activation was
reduced in the presence of `` dominant negative ''
Raf1 ( RafS621A ) and slightly reduced by dominant negative B-Raf, indicating the involvement of one or more Raf isoforms
Miura et al., Biochem Biophys Res Commun 2003
:
These results demonstrate that
Ras/Raf1 dependent
ERK activation mediated by PTX-sensitive G protein coupled receptors may be a potent signal in S1P induced HCEC tube formation
Sippel et al., Am J Physiol Gastrointest Liver Physiol 2003
(Carcinoid Tumor...) :
Activation of
raf-1 in BON-raf cells
led to a marked induction of phosphorylated MEK and
ERK1/2 within 48 h. Importantly, raf-1 activation resulted in morphological changes accompanied by a marked decrease in neuroendocrine secretory granules by electronmicroscopy ... Furthermore, treatment of BON-raf cells with MEK inhibitors PD-98059 and U-0126 blocked
raf-1 mediated morphological changes and hormone suppression but not
ERK1/2 phosphorylation
Cerioni et al., FEBS Lett 2003
(Adrenal Gland Neoplasms...) :
These results, along with others obtained using respiratory chain inhibitors and respiration-deficient cells, lead to the conclusion that PD98059, while effectively inhibiting the peroxynitrite induced Raf/MEK signaling leading to ERK1/2 phosphorylation, promotes an enforced mitochondrial formation of ROS inducing
ERK1/2 phosphorylation via a
Raf-1-independent/MEK dependent mechanism
Illario et al., J Biol Chem 2003
:
This is the first demonstration that CaMKII interplays with Raf-1 and regulates
Erk activation
induced by Ras stimulated
Raf-1
Kamata et al., Blood 2004
(Thrombocytopenia) :
Raf-1 is not
required for megakaryocytopoiesis or TPO induced
ERK phosphorylation
Sippel et al., Surgery 2003
(Carcinoma, Medullary...) :
Activation of
raf-1 in the TT-raf cells
resulted in high levels of phosphorylated MEK and
ERK1/2 , a morphologic transdifferentiation, and a decrease in chromogranin A and calcitonin levels that are associated with a reduction in hASH1 production
Clark et al., J Biol Chem 2004
(MAP Kinase Signaling System) :
PKC/PKC alpha activity promoted GTP loading of Ras,
activation of
Raf-1 , and phosphorylation/activation of
ERK
Su et al., J Biol Chem 2004
(Chlamydia Infections) :
Blocking either
c-Raf-1 or MEK1/2 activity
prevented the chlamydial activation of
ERK1/2 , leading to the suppression of both chlamydial activation of the host cPLA2 and uptake of glycerophospholipids from the host cells
Schattenberg et al., Hepatology 2004
(MAP Kinase Signaling System) :
CYP2E1 expression
induced ERK1/2 activation through increased epidermal growth factor receptor
(EGFR)/c-Raf signaling
Rössler et al., J Neurochem 2004
:
Activation of this conditionally active form of
Raf-1 induced a sustained phosphorylation of
ERK , and protected the cells from serum withdrawal induced cell death
Keller et al., Biochem Pharmacol 2004
(Neoplasms) :
Protein kinase C ( PKC ) phosphorylates RKIP, resulting in release of
Raf-1 and
activation of MEK and
ERK
Torres et al., Nephron. Experimental nephrology 2004
(Polycystic Kidney Diseases) :
While cAMP inhibits
Ras-Raf-1 stimulated phosphorylation of
ERK in normal kidney cells, it markedly increases B-Raf kinase activity and ERK phosphorylation in polycystic kidney cells
Wang et al., J Cell Physiol 2005
(Liver Neoplasms, Experimental...) :
The suppression of
Raf-1 Ser-259 phosphorylation
caused the induction of Raf-1 kinase activity, as well as
hyper-ERK phosphorylation
Krepinsky et al., Cell Signal 2005
:
The
Raf-1 inhibitor, GW5074, dose-dependently
blocked strain induced
Erk activation and Raf-1 phosphorylation
Mariappan et al., J Biol Chem 2005
:
Studies with PP1/2 showed that phosphorylation of c-Src was required for tyrosine phosphorylation of
Raf-1 , an upstream
regulator of
Erk
Calipel et al., J Biol Chem 2006
(Cell Transformation, Neoplastic...) :
siRNA mediated depletion of
Raf-1 did not
affect either
ERK1/2 activation, whereas siRNA mediated depletion of B-Raf reduced cell proliferation by up to 65 % through the inhibition of ERK1/2 activation, irrespective of the mutational status of B-Raf
Raines et al., Biochem J 2006
:
In contrast, exogenous expression of constitutively active mutants of
Raf-1 and H-Ras only partially
restored ERK1/2 activity, by 50 % and 10 % respectively
Sánchez-Tilló et al., J Immunol 2006
:
However, whereas
ERK phosphorylation in response to M-CSF is
Raf-1 dependent , in response to LPS, an alternative pathway directs the activation of these kinases
Lee et al., Am J Physiol Heart Circ Physiol 2006
(Inflammation...) :
However, inhibitors of
Raf-1 and MEK or a dominant negative ERK mutant
blocked FKN induced
ERK , but not Akt and eNOS, phosphorylation
Wang et al., J Cell Physiol 2006
:
In late stage differentiating cells
Raf-1 and p90RSK are found as a complex, and inhibition of Raf-1, but not MEK or
ERK expression
reduces the levels of phosphorylated p90 RSK
Marzec et al., Oncogene 2007
(MAP Kinase Signaling System) :
The NPM/ALK induced
MEK/ERK activation is
independent of
c-Raf as evidenced by the lack of MEK1/2 and ERK1/2 phosphorylation upon c-Raf inactivation by two different inhibitors, RI and ZM336372, and by its siRNA mediated depletion
Kohda et al., J Toxicol Sci 2006
:
The
Raf-1 kinase inhibitor and U0126 also
suppressed ERK1/2 activation in nuclear fractions prepared from slices treated with zinc
Kogut et al., Mol Immunol 2007
:
The classic Ras mediated pathway of ERK1/2 activation by LPS was confirmed when the specific
Raf-1 inhibitor, GW 5074, and the MEK1/2 inhibitor, U0126, both reduced
ERK activation by 51-60 %
Kanno et al., J Cell Biochem 2007
(MAP Kinase Signaling System) :
These findings indicate that mechanical stress regulates Runx2 activation and favors osteoblast differentiation through the activation of MAPK signal transduction pathways and
Ras/Raf dependent
ERK1/2 activation, independent of p38 MAPK signaling
Golding et al., Cancer Res 2007
(Glioma...) :
When
ERK1/2 signaling was
stimulated by oncogenic
RAF-1 , an approximately 2-fold increase in HRR was observed
Morishita et al., Neurosci Res 2007
:
Endothelin induced
ERK phosphorylation was independent of integrin ligands, and an inhibitor of G ( q/11 ), YM-254890, as well as pertussis toxin, partially
inhibited endothelin stimulated phosphorylation of
Raf-1 and ERK
Galabova-Kovacs et al., J Cell Biol 2008
(MAP Kinase Signaling System) :
In B-Raf-deficient cells, more
Raf-1 is recruited to MEK, yet
MEK/ERK phosphorylation is
impaired
Gerits et al., Cell Signal 2008
:
The first- and best characterized interconnections are the PKA dependent inhibition of the MAPKs
ERK1/2 mediated by
RAF-1 , and PKA induced activation of ERK1/2 interceded through B-RAF
Lai et al., Cell Biochem Funct 2009
:
Our results suggest that rapid PTH inhibition of
ERK1/2 activity is
mediated by PKA dependent inhibition of
c-Raf activity and that stimulation of MKP-1 may contribute to maintaining pERK1/2 inhibition over prolonged time
Zhao et al., Proc Natl Acad Sci U S A 2011
(Asthma...) :
MAPK kinase ( MEK )
/ERK activation is
regulated by interactions of
Raf-1 with phosphatidylethanolamine binding protein 1 ( PEBP1 ) ... siRNA knockdown of 15LO1 decreases the dissociation of
Raf-1 from PEBP1, and the resulting lower
ERK activation leads to lower downstream IL-4Ra related gene expression
Mor et al., Am J Physiol Cell Physiol 2012
(MAP Kinase Signaling System) :
Coexpression of ZnT-1 and nonactive
Raf-1 blocked the ZnT-1 mediated
ERK phosphorylation and abolished the ZnT-1 induced augmentation of I ( caT )
Hutchison et al., Mol Endocrinol 2012
(MAP Kinase Signaling System) :
This work describes a model wherein unopposed IGF-I promotes high ERK/p38 activity ratios favoring proliferation, whereas BDNF signals a transition to differentiation by decreasing the ERK/p38 activity ratio without completely inhibiting
ERK , which
involves the direct inhibition of
raf-1 by p38
Amatangelo et al., Carcinogenesis 2012
(Prostatic Neoplasms) :
Mechanistic studies revealed that in cells stably transfected with activated Ras, TGF-ß alone induced EMT and that a
Ras-Raf-MEK1 , but not MEK2, signaling cascade is necessary and
sufficient for
Erk2 nuclear localization that works in concert with TGF-ß to promote EMT
Wu et al., Mol Cell Biol 2012
(MAP Kinase Signaling System...) :
Furthermore, kinase activating
RAF1 mutants also
required heterodimerization to enhance
MEK/ERK activation
Atabakhsh et al., PloS one 2012
(Cell Transformation, Neoplastic...) :
Activation of
ERK by active
c-Raf was also prevented by RanBPM
Li et al., Cell Physiol Biochem 2012
(Acute Lung Injury...) :
Inhibition of Src or
Raf-1 blocked LPS induced
ERK activation
Deswal et al., J Immunol 2013
:
Although
Raf-1 triggers transient
Erk activation, B-Raf is implicated in sustained Erk signaling after TCR stimulation
Blume-Jensen et al., J Biol Chem 1994
:
SCF induced phosphorylation of
Raf-1 and
activation of
ERK2 still occurred after PKC inhibition but was not increased
Lisbona et al., Eur J Immunol 1994
:
We also show that ERK kinase activation precedes Raf-1 kinase hyperphosphorylation, suggesting that
Raf-1 kinase activation is not
required for
ERK kinase activation
Minden et al., Science 1994
:
Raf-1 contributes directly to
ERK activation but not to JNK activation, whereas MEKK participated in JNK activation but caused ERK activation only after overexpression
Izquierdo et al., J Exp Med 1994
:
In the present study, the
role of
Raf-1 in
ERK2 regulation of ERK2 in T cells has been explored ... These data indicate a
role for
Raf-1 in the regulation of
ERK2 in T cells
Marrero et al., J Biol Chem 1997
:
AG-490, a specific inhibitor of the JAK2 tyrosine kinase, prevented proliferation of vascular smooth muscle cells, complex formation between JAK2 and Raf-1, the tyrosine phosphorylation of
Raf-1 , and the
activation of
ERK1 in response to either angiotensin II or platelet derived growth factor
Carson-Walter et al., Oncogene 1998
(Carcinoma, Medullary...) :
We now show that
raf-1 induction is
followed by activation of the downstream kinases MEK1/2 and
ERK1/2 and that differentiation is dependent on activation of MEK1/2
Florio et al., Mol Endocrinol 1999
:
We show that the activation of
ERK via SSTR1 is pertussis toxin sensitive and
requires the small G protein Ras, phosphatidylinositol 3-kinase, the serine/threonine kinase
Raf-1 , and the protein tyrosine phosphatase SHP-2