J Biol Chem 1996,
PMID: 8702662
Bubeck Wardenburg, J; Fu, C; Jackman, J K; Flotow, H; Wilkinson, S E; Williams, D H; Johnson, R; Kong, G; Chan, A C; Findell, P R
Two families of tyrosine kinases, the Src and Syk families, are required for T-cell receptor activation. While the Src kinases are responsible for phosphorylation of receptor-encoded signaling motifs and for up-regulation of ZAP-70 activity, the downstream substrates of ZAP-70 are unknown. Evidence is presented herein that the Src homology 2 (SH2) domain-containing leukocyte protein of 76 kDa (SLP-76) is a substrate of ZAP-70. Phosphorylation of SLP-76 is diminished in T cells that express a catalytically inactive ZAP-70. Moreover, SLP-76 is preferentially phosphorylated by ZAP-70 in vitro and in heterologous cellular systems. In T cells, overexpression of wild-type SLP-76 results in a hyperactive receptor, while expression of a SLP-76 molecule that is unable to be tyrosine-phosphorylated attenuates receptor function. In addition, the SH2 domain of SLP-76 is required for T-cell receptor function, although its role is independent of the ability of SLP-76 to undergo tyrosine phosphorylation. As SLP-76 interacts with both Grb2 and phospholipase C-gamma1, these data indicate that phosphorylation of SLP-76 by ZAP-70 provides an important functional link between the T-cell receptor and activation of ras and calcium pathways.
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Manually curated Databases
-
IRef Hprd Interaction:
ZAP70
—
LCP2
(in vitro)
-
IRef Hprd Interaction:
ZAP70
—
LCP2
(in vivo)
-
IRef Hprd Interaction:
LCK
—
LCP2
(in vitro)
-
IRef Hprd Interaction:
LCK
—
LCP2
(in vivo)
-
IRef Hprd Interaction:
TXK
—
LCP2
(in vivo)
-
IRef Hprd Interaction:
TXK
—
LCP2
(in vitro)
-
Reactome Reaction:
GRAP2
→
IGLC3
(reaction)
-
Reactome Reaction:
IGHE
→
SOS1
(reaction)
-
Reactome Reaction:
IGKV1-5
→
SOS1
(reaction)
-
Reactome Reaction:
GRB2
→
HLA-E
(reaction)
-
Reactome Reaction:
KLRC2
→
LAT
(reaction)
-
Reactome Reaction:
IGKV4-1
→
PLCG1
(reaction)
-
Reactome Reaction:
FYN
→
PLCG2
(reaction)
-
Reactome Reaction:
B2M
→
PLCG2
(reaction)
-
Reactome Reaction:
KLRK1
→
SOS1
(reaction)
-
Reactome Reaction:
LCP2
→
SHC1
(indirect_complex)
-
Reactome Reaction:
GRB2
→
IGLC2
(reaction)
-
Reactome Reaction:
LYN
→
SOS1
(reaction)
-
Reactome Reaction:
GRAP2
→
KLRC2
(reaction)
-
Reactome Reaction:
GRB2
→
IGLC6
(reaction)
-
Reactome Reaction:
LCP2
→
KLRC2
(reaction)
-
Reactome Reaction:
LCP2
→
PLCG2
(indirect_complex)
-
Reactome Reaction:
IGKV4-1
→
PLCG2
(reaction)
-
Reactome Reaction:
IGKV4-1
→
SOS1
(reaction)
-
Reactome Reaction:
SHC1
→
IGLC1
(reaction)
-
Reactome Reaction:
IGKC
→
PLCG2
(reaction)
-
Reactome Reaction:
B2M
→
LAT
(reaction)
-
Reactome Reaction:
PLCG1
→
IGLC7
(reaction)
-
Reactome Reaction:
LYN
→
PLCG2
(reaction)
-
Reactome Reaction:
B2M
→
SHC1
(reaction)
-
Reactome Reaction:
LYN
→
SHC1
(reaction)
-
Reactome Reaction:
LCK
→
LCP2
(reaction)
-
Reactome Reaction:
GRB2
→
KLRK1
(reaction)
-
Reactome Reaction:
PLCG2
→
IGLC3
(reaction)
-
Reactome Reaction:
SHC1
→
IGLC7
(reaction)
-
Reactome Reaction:
SYK
→
PLCG1
(reaction)
-
Reactome Reaction:
LCP2
→
LAT
(indirect_complex)
-
Reactome Reaction:
LCK
→
PLCG2
(reaction)
-
Reactome Reaction:
IGHE
→
GRAP2
(reaction)
-
Reactome Reaction:
LCK
→
PLCG1
(reaction)
-
Reactome Reaction:
FYN
→
LAT
(reaction)
-
Reactome Reaction:
IGHE
→
SHC1
(reaction)
-
Reactome Reaction:
IGKC
→
LCP2
(reaction)
-
Reactome Reaction:
LAT
→
TREM2
(reaction)
-
Reactome Reaction:
LAT
→
IGLC6
(reaction)
-
Reactome Reaction:
GRAP2
→
IGLC2
(reaction)
-
Reactome Reaction:
SYK
→
LAT
(reaction)
-
Reactome Reaction:
PLCG2
→
IGLC1
(reaction)
-
Reactome Reaction:
PLCG1
→
TREM2
(reaction)
-
Reactome Reaction:
GRB2
→
KLRC2
(reaction)
-
Reactome Reaction:
LCP2
→
SOS1
(indirect_complex)
-
Reactome Reaction:
GRB2
→
KLRD1
(reaction)
-
Reactome Reaction:
B2M
→
SOS1
(reaction)
-
Reactome Reaction:
IGHE
→
GRB2
(reaction)
-
Reactome Reaction:
KLRK1
→
PLCG2
(reaction)
-
Reactome Reaction:
IGHE
→
PLCG2
(reaction)
-
Reactome Reaction:
PLCG2
→
KLRD1
(reaction)
-
Reactome Reaction:
FYN
→
SOS1
(reaction)
-
Reactome Reaction:
B2M
→
GRB2
(reaction)
-
Reactome Reaction:
IGKV1-5
→
LCP2
(reaction)
-
Reactome Reaction:
SOS1
→
IGLC7
(reaction)
-
Reactome Reaction:
HLA-E
→
PLCG2
(reaction)
-
Reactome Reaction:
PLCG2
→
SHC1
(indirect_complex)
-
Reactome Reaction:
GRAP2
→
PLCG2
(indirect_complex)
-
Reactome Reaction:
LAT
→
IGLC1
(reaction)
-
Reactome Reaction:
PLCG2
→
IGLC6
(reaction)
-
Reactome Reaction:
GRAP2
→
IGKV4-1
(reaction)
-
Reactome Reaction:
IGKV1-5
→
PLCG2
(reaction)
-
Reactome Reaction:
HLA-E
→
LAT
(reaction)
-
Reactome Reaction:
TYROBP
→
SHC1
(reaction)
-
Reactome Reaction:
FYN
→
SHC1
(reaction)
-
Reactome Reaction:
SHC1
→
IGLC3
(reaction)
-
Reactome Reaction:
IGHE
→
LAT
(reaction)
-
Reactome Reaction:
HLA-E
→
LCP2
(reaction)
-
Reactome Reaction:
LAT
→
KLRD1
(reaction)
-
Reactome Reaction:
LCK
→
SOS1
(reaction)
-
Reactome Reaction:
PLCG2
→
IGLC2
(reaction)
-
Reactome Reaction:
FYN
→
GRB2
(reaction)
-
Reactome Reaction:
HLA-E
→
PLCG1
(reaction)
-
Reactome Reaction:
LCP2
→
LYN
(reaction)
-
Reactome Reaction:
SOS1
→
IGLC3
(reaction)
-
Reactome Reaction:
GRB2
→
IGKV1-5
(reaction)
-
Reactome Reaction:
GRAP2
→
LCK
(reaction)
-
Reactome Reaction:
PLCG1
→
SOS1
(indirect_complex)
-
Reactome Reaction:
IGKV4-1
→
LAT
(reaction)
-
Reactome Reaction:
GRAP2
→
LYN
(reaction)
-
Reactome Reaction:
IGKV4-1
→
LCP2
(reaction)
-
Reactome Reaction:
B2M
→
LCP2
(reaction)
-
Reactome Reaction:
SOS1
→
IGLC2
(reaction)
-
Reactome Reaction:
GRAP2
→
IGLC7
(reaction)
-
Reactome Reaction:
SYK
→
SOS1
(reaction)
-
Reactome Reaction:
KLRC2
→
SOS1
(reaction)
-
Reactome Reaction:
LAT
→
IGLC2
(reaction)
-
Reactome Reaction:
GRAP2
→
TREM2
(reaction)
-
Reactome Reaction:
IGKV1-5
→
PLCG1
(reaction)
-
Reactome Reaction:
LCP2
→
KLRD1
(reaction)
-
Reactome Reaction:
PLCG1
→
LAT
(indirect_complex)
-
Reactome Reaction:
GRAP2
→
IGKV1-5
(reaction)
-
Reactome Reaction:
GRB2
→
IGKC
(reaction)
-
Reactome Reaction:
IGKV1-5
→
LAT
(reaction)
-
Reactome Reaction:
SHC1
→
IGLC2
(reaction)
-
Reactome Reaction:
SOS1
→
KLRD1
(reaction)
-
Reactome Reaction:
PLCG2
→
SOS1
(indirect_complex)
-
Reactome Reaction:
SHC1
→
IGLC6
(reaction)
-
Reactome Reaction:
FYN
→
GRAP2
(reaction)
-
Reactome Reaction:
GRB2
→
IGLC7
(reaction)
-
Reactome Reaction:
LCK
→
LAT
(reaction)
-
Reactome Reaction:
B2M
→
PLCG1
(reaction)
-
Reactome Reaction:
GRAP2
→
IGKC
(reaction)
-
Reactome Reaction:
FYN
→
PLCG1
(reaction)
-
Reactome Reaction:
B2M
→
GRAP2
(reaction)
-
Reactome Reaction:
TREM2
→
SHC1
(reaction)
-
Reactome Reaction:
LCP2
→
PLCG1
(indirect_complex)
-
Reactome Reaction:
LCP2
→
IGLC7
(reaction)
-
Reactome Reaction:
SYK
→
PLCG2
(reaction)
-
Reactome Reaction:
SOS1
→
TYROBP
(reaction)
-
Reactome Reaction:
PLCG1
→
IGLC6
(reaction)
-
Reactome Reaction:
IGKC
→
PLCG1
(reaction)
-
Reactome Reaction:
LCP2
→
TYROBP
(reaction)
-
Reactome Reaction:
GRB2
→
SYK
(reaction)
-
Reactome Reaction:
GRAP2
→
KLRD1
(reaction)
-
Reactome Reaction:
LYN
→
PLCG1
(reaction)
-
Reactome Reaction:
SYK
→
SHC1
(reaction)
-
Reactome Reaction:
GRB2
→
IGLC1
(reaction)
-
Reactome Reaction:
GRB2
→
LCK
(reaction)
-
Reactome Reaction:
GRAP2
→
LAT
(indirect_complex)
-
Reactome Reaction:
LCP2
→
IGLC1
(reaction)
-
Reactome Reaction:
GRB2
→
IGKV4-1
(reaction)
-
Reactome Reaction:
LCP2
→
TREM2
(reaction)
-
Reactome Reaction:
SHC1
→
KLRD1
(reaction)
-
Reactome Reaction:
SYK
→
LCP2
(reaction)
-
Reactome Reaction:
HLA-E
→
SOS1
(reaction)
-
Reactome Reaction:
IGKV4-1
→
SHC1
(reaction)
-
Reactome Reaction:
HLA-E
→
SHC1
(reaction)
-
Reactome Reaction:
GRB2
→
TYROBP
(reaction)
-
Reactome Reaction:
GRAP2
→
IGLC1
(reaction)
-
Reactome Reaction:
TYROBP
→
LAT
(reaction)
-
Reactome Reaction:
KLRC2
→
PLCG1
(reaction)
-
Reactome Reaction:
LAT
→
IGLC3
(reaction)
-
Reactome Reaction:
IGKC
→
SHC1
(reaction)
-
Reactome Reaction:
KLRK1
→
PLCG1
(reaction)
-
Reactome Reaction:
KLRK1
→
LAT
(reaction)
-
Reactome Reaction:
GRAP2
→
SYK
(reaction)
-
Reactome Reaction:
IGKC
→
LAT
(reaction)
-
Reactome Reaction:
LYN
→
LAT
(reaction)
-
Reactome Reaction:
GRAP2
→
HLA-E
(reaction)
-
Reactome Reaction:
GRAP2
→
KLRK1
(reaction)
-
Reactome Reaction:
IGKC
→
SOS1
(reaction)
-
Reactome Reaction:
FYN
→
LCP2
(reaction)
-
Reactome Reaction:
KLRC2
→
PLCG2
(reaction)
-
Reactome Reaction:
GRB2
→
GRAP2
(indirect_complex)
-
Reactome Reaction:
PLCG1
→
IGLC2
(reaction)
-
Reactome Reaction:
LAT
→
IGLC7
(reaction)
-
Reactome Reaction:
GRB2
→
LCP2
(indirect_complex)
-
Reactome Reaction:
LCP2
→
IGLC3
(reaction)
-
Reactome Reaction:
GRB2
→
IGLC3
(reaction)
-
Reactome Reaction:
PLCG1
→
TYROBP
(reaction)
-
Reactome Reaction:
GRAP2
→
PLCG1
(indirect_complex)
-
Reactome Reaction:
PLCG1
→
IGLC3
(reaction)
-
Reactome Reaction:
PLCG1
→
IGLC1
(reaction)
-
Reactome Reaction:
IGHE
→
LCP2
(reaction)
-
Reactome Reaction:
LCP2
→
IGLC6
(reaction)
-
Reactome Reaction:
SOS1
→
TREM2
(reaction)
-
Reactome Reaction:
PLCG2
→
LAT
(indirect_complex)
-
Reactome Reaction:
PLCG1
→
KLRD1
(reaction)
-
Reactome Reaction:
PLCG2
→
IGLC7
(reaction)
-
Reactome Reaction:
LCK
→
SHC1
(reaction)
-
Reactome Reaction:
IGKV1-5
→
SHC1
(reaction)
-
Reactome Reaction:
LCP2
→
IGLC2
(reaction)
-
Reactome Reaction:
KLRK1
→
SHC1
(reaction)
-
Reactome Reaction:
PLCG2
→
TREM2
(reaction)
-
Reactome Reaction:
LCP2
→
KLRK1
(reaction)
-
Reactome Reaction:
KLRC2
→
SHC1
(reaction)
-
Reactome Reaction:
GRB2
→
TREM2
(reaction)
-
Reactome Reaction:
GRAP2
→
SOS1
(indirect_complex)
-
Reactome Reaction:
SOS1
→
IGLC1
(reaction)
-
Reactome Reaction:
GRAP2
→
SHC1
(indirect_complex)
-
Reactome Reaction:
PLCG1
→
SHC1
(indirect_complex)
-
Reactome Reaction:
IGHE
→
PLCG1
(reaction)
-
Reactome Reaction:
PLCG2
→
TYROBP
(reaction)
-
Reactome Reaction:
GRAP2
→
IGLC6
(reaction)
-
Reactome Reaction:
GRB2
→
LYN
(reaction)
-
Reactome Reaction:
GRAP2
→
TYROBP
(reaction)
-
Reactome Reaction:
GRB2
→
PLCG1
(indirect_complex)
-
Reactome Reaction:
SOS1
→
IGLC6
(reaction)
-
Reactome Reaction:
GRB2
→
PLCG2
(indirect_complex)
In total, 182 gene pairs are associated to this article in curated databases