Blood 1993,
PMID: 7678512
Takahashi, G W; Andrews, D F; Lilly, M B; Singer, J W; Alderson, M R
Interleukin-8 (IL-8) is a major neutrophil chemoattractant and functional stimulant that is induced by IL-1, tumor necrosis factor alpha (TNF alpha), and lipopolysaccharide (LPS). We report that recombinant human (rh) granulocyte-macrophage colony-stimulating factor (GM-CSF) and rhIL-3 are also potent inducers of IL-8 messenger RNA (mRNA) accumulation and protein secretion by normal peripheral blood monocytes. Neutrophils produce IL-8 in response to GM-CSF but not to IL-3. In contrast, recombinant human granulocyte-CSF (rhG-CSF), at concentrations as high as 100 ng/mL, does not induce IL-8 in either cell type. rhGM-CSF also induces IL-8 mRNA expression and IL-8 protein in the promonocytic cell line, U-937, whereas rhG-CSF does not. IL-8 secretion by monocytes was stimulated within 2 hours after incubation with rhGM-CSF or rhIL-3. Stimulation of neutrophils with rhGM-CSF resulted in an increase in cell-associated IL-8 at 4 hours. At 24 hours, cell-associated IL-8 levels declined, whereas secreted IL-8 levels increased. In contrast, virtually all IL-8 induced in monocytes appeared as secreted protein. Neither rhGM-CSF nor rhIL-3 induced detectable secretion of IL-1, TNF alpha, or IL-6 protein by monocytes. rhGM-CSF, and to a lesser degree rhIL-3, potently stimulated IL-8 secretion in cultures of heparinized whole blood, whereas rhG-CSF had no significant effect on IL-8 secretion. Induction of IL-8 by GM-CSF may be physiologically important in enhancing the acute inflammatory response.
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Text Mining Data
interleukin-8 — colony stimulating factor: "
Effect of granulocyte-macrophage
colony stimulating factor and interleukin-3 on
interleukin-8 production by human neutrophils and monocytes
"
interleukin-8 — interleukin-3: "
Effect of granulocyte-macrophage colony stimulating factor and interleukin-3 on interleukin-8 production by human neutrophils and monocytes
"
IL-8 → GM-CSF: "
Neutrophils produce IL-8 in response to GM-CSF but not to IL-3
"
granulocyte-CSF → IL-8: "
In contrast, recombinant human granulocyte-CSF ( rhG-CSF ), at concentrations as high as 100 ng/mL, does not induce IL-8 in either cell type
"
rhGM-CSF → IL-8: "
rhGM-CSF also induces IL-8 mRNA expression and IL-8 protein in the promonocytic cell line, U-937, whereas rhG-CSF does not
"
IL-8 ⊣ rhGM-CSF: "
Stimulation of neutrophils with rhGM-CSF resulted in an increase in cell associated IL-8 at 4 hours
"
IL-8 → rhG-CSF: "
rhGM-CSF, and to a lesser degree rhIL-3, potently stimulated IL-8 secretion in cultures of heparinized whole blood, whereas rhG-CSF had no significant effect on IL-8 secretion
"
IL-8 → GM-CSF: "
Induction of IL-8 by GM-CSF may be physiologically important in enhancing the acute inflammatory response
"
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