Biol Trace Elem Res 2012,
PMID: 22576985
Matsebatlela, Thabe; Gallicchio, Vincent; Becker, Rolf
Lithium has been an FDA-approved and preferred drug for the treatment of mood disorders for many years, and cumulative evidence has pointed towards its potential use as an anti-cancer agent. Previous studies in our laboratory have demonstrated that lithium induces apoptotic cell death in HL-60 promyelocytes at concentrations of 10 mM and above. A lithium-tolerant HL-60 sub-clone, resistant to up to 15 mM lithium, was also generated and its growth profile reported. Treatment of cells with lithium resulted in a dose-dependent induction of p53, retinoblastoma (Rb) and bax expression which was accompanied by concomitant inhibition of bcl-2 expression as demonstrated using immunohistochemical microscopy. These results seem to suggest that lithium induced cell death in these cells by inhibiting expression of anti-apoptotic protein, bcl-2, while inducing higher expression of its pro-apoptotic counterparts which include bax. Expression of bax and bcl-2 is also linked to expression of inflammation-regulating cytokines. Using ELISA assays, lithium was demonstrated to induce production of pro-inflammatory cytokines, IL-6 and TNF-α, while inhibiting release of anti-inflammation-related IL-2 and IL-10 in a dose-dependent fashion. Our findings identify a critical function for lithium in modulating pro- versus anti-apoptotic gene expression and pro- versus anti-inflammatory cytokines in vitro and provide a rationale for suggesting a promising role of lithium in regulation of inflammation and cancer growth.
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Text Mining Data
TNF-a ⊣ IL-10: "
Using ELISA assays, lithium was demonstrated to
induce production of pro-inflammatory cytokines, IL-6 and
TNF-a , while inhibiting release of anti-inflammation related IL-2 and
IL-10 in a dose dependent fashion
"
TNF-a ⊣ IL-2: "
Using ELISA assays, lithium was demonstrated to induce production of pro-inflammatory cytokines, IL-6 and TNF-a , while inhibiting release of anti-inflammation related IL-2 and IL-10 in a dose dependent fashion
"
IL-6 ⊣ IL-10: "
Using ELISA assays, lithium was demonstrated to induce production of pro-inflammatory cytokines, IL-6 and TNF-a, while inhibiting release of anti-inflammation related IL-2 and IL-10 in a dose dependent fashion
"
IL-6 ⊣ IL-2: "
Using ELISA assays, lithium was demonstrated to induce production of pro-inflammatory cytokines, IL-6 and TNF-a, while inhibiting release of anti-inflammation related IL-2 and IL-10 in a dose dependent fashion
"
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