Gene interactions and pathways from curated databases and text-mining
Inflamm Res 2009, PMID: 19214384

Inhibitory effect of ZPDC glycoprotein on the expression of inflammation-related cytokines through p38 MAP kinase and JNK in lipopolysaccharide-stimulated RAW 264.7 cells.

Lee, S-J; Lim, K-T

OBJECTIVE

This study was carried out to investigate the anti-inflammatory potentials of 24 kDa glycoprotein isolated from Zanthoxylum piperitum DC fruit (ZPDC glycoprotein) in lipopolysaccharide (LPS)-stimulated murine macrophage cell line (RAW 264.7 cells).

METHODS

RAW 264.7 cells were treated with ZPDC glycoprotein (50-200 microg/ml) in presence of LPS (2 microg/ml). The changes of the levels of inflammation-related factors were determined by using Western blot, EMSA, and RT-PCR.

RESULTS

ZPDC glycoprotein has inhibitory effects on the phosphorylation of p38 mitogen-activated protein (MAP) kinase and c-Jun N-terminal kinase (JNK), on the DNA binding activity of activator protein-1 (AP-1), and on the expression of c-Jun and c-Fos in LPS-stimulated RAW 264.7 cells. Interestingly, the DNA binding activity of AP-1 was attenuated by treatment with inhibitors of p38 MAP kinase and JNK. In addition, ZPDC glycoprotein (200 microg/ml) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines (IL-1 beta, IL-6, and TNF-alpha) and proteins (iNOS, COX-2, and MMP-9) in LPS- stimulated RAW 264.7 cells.

CONCLUSIONS

The present study demonstrates that ZPDC glycoprotein is a natural anti-oxidant and one of the modulators of pro-inflammatory signal transduction pathways in RAW 264.7 cells.

Diseases/Pathways annotated by Medline MESH: Inflammation, Second Messenger Systems
Document information provided by NCBI PubMed

Text Mining Data

TNF-alpha ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta, IL-6, and TNF-alpha ) and proteins ( iNOS, COX-2, and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

IL-1 beta ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta , IL-6, and TNF-alpha ) and proteins ( iNOS, COX-2, and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

IL-6 ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta, IL-6 , and TNF-alpha ) and proteins ( iNOS, COX-2, and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

MMP-9 ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta, IL-6, and TNF-alpha ) and proteins ( iNOS, COX-2, and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

iNOS ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta, IL-6, and TNF-alpha ) and proteins ( iNOS , COX-2, and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

COX-2 ⊣ glycoprotein: " In addition, ZPDC glycoprotein ( 200 microg/ml ) not only diminished the production of superoxide anion, hydrogen peroxide, and nitric oxide, but also suppressed the expression of pro-inflammatory cytokines ( IL-1 beta, IL-6, and TNF-alpha ) and proteins ( iNOS, COX-2 , and MMP-9 ) in LPS- stimulated RAW 264.7 cells "

Manually curated Databases

No curated data.