Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2008, PMID: 18838386

Human ATAC Is a GCN5/PCAF-containing acetylase complex with a novel NC2-like histone fold module that interacts with the TATA-binding protein.

Wang, Yuan-Liang; Faiola, Francesco; Xu, Muyu; Pan, Songqin; Martinez, Ernest

Eukaryotic GCN5 acetyltransferases influence diverse biological processes by acetylating histones and non-histone proteins and regulating chromatin and gene-specific transcription as part of multiprotein complexes. In lower eukaryotes and invertebrates, these complexes include the yeast ADA complex that is still incompletely understood; the SAGA (Spt-Ada-Gcn5 acetylase) complexes from yeast to Drosophila that are mostly coactivators; and the ATAC (Ada Two-A containing) complex, only known in Drosophila and still poorly characterized. In contrast, vertebrate organisms, express two paralogous GCN5-like acetyltransferases (GCN5 and PCAF), which have been found so far only in SAGA-type complexes referred to hereafter as the STAGA (SPT3-TAF9-GCN5/PCAF acetylase) complexes. We now report the purification and characterization of vertebrate (human) ATAC-type complexes and identify novel components of STAGA. We show that human ATAC complexes incorporate in addition to GCN5 or PCAF (GCN5/PCAF), other epigenetic coregulators (ADA2-A, ADA3, STAF36, and WDR5), cofactors of chromatin assembly/remodeling and DNA replication machineries (POLE3/CHRAC17 and POLE4), the stress- and TGFbeta-activated protein kinase (TAK1/MAP3K7) and MAP3-kinase regulator (MBIP), additional cofactors of unknown function, and a novel YEATS2-NC2beta histone fold module that interacts with the TATA-binding protein (TBP) and negatively regulates transcription when recruited to a promoter. We further identify the p38 kinase-interacting protein (p38IP/FAM48A) as a novel component of STAGA with distant similarity to yeast Spt20. These results suggest that vertebrate ATAC-type and STAGA-type complexes link specific extracellular signals to modification of chromatin structure and regulation of the basal transcription machinery.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: WDR5 — TADA2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DRAP1 — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA3 — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — SUPT3H (colocalization, biochemical)
  • IRef Biogrid Interaction: CCDC101 — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — UBAP2L (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DR1 — SUPT20H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HCFC1 — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DR1 — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: POLE4 — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCDC101 — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2B — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — CSRP2BP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ZZZ3 — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — POLE3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — KAT2B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2B — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — KAT2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2A — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCDC101 — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCDC101 — MAP3K7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2A — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2B — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCDC101 — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — MAP3K7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2B — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA2A — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — YEATS2 (colocalization, biochemical)
  • IRef Biogrid Interaction: KAT2A — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DR1 — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — KAT2B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — TADA3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — TADA3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA3 — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — DR1 (colocalization, biochemical)
  • IRef Biogrid Interaction: KAT2A — DR1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: YEATS2 — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TADA3 — MAP3K7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DR1 — MAP3K7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — YEATS2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CCDC101 — WDR5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DR1 — TADA3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — MAP3K7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — DRAP1 (colocalization, biochemical)
  • IRef Biogrid Interaction: WDR5 — WDR5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KAT2A — KAT2A (colocalization, biochemical)
  • IRef Biogrid Interaction: TRRAP — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MBIP — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — MBIP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TAF12 — SUPT3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAP3K7 — MAP3K7 (physical association, affinity chromatography technology)
  • Gene Ontology Complexes SAGA-type complex: SAGA-type complex complex (TADA2B-TADA1-USP22)
  • Gene Ontology Complexes Ada2/Gcn5/Ada3 transcription activator complex: Ada2/Gcn5/Ada3 transcription activator complex complex (WDR5-ZZZ3-UBAP2L-DR1-KAT2B-KAT2A-YEATS2-POLE4-POLE3-TADA3-CSRP2BP)
In total, 95 gene pairs are associated to this article in curated databases