J Biol Chem 2008,
PMID: 18838386
Wang, Yuan-Liang; Faiola, Francesco; Xu, Muyu; Pan, Songqin; Martinez, Ernest
Eukaryotic GCN5 acetyltransferases influence diverse biological processes by acetylating histones and non-histone proteins and regulating chromatin and gene-specific transcription as part of multiprotein complexes. In lower eukaryotes and invertebrates, these complexes include the yeast ADA complex that is still incompletely understood; the SAGA (Spt-Ada-Gcn5 acetylase) complexes from yeast to Drosophila that are mostly coactivators; and the ATAC (Ada Two-A containing) complex, only known in Drosophila and still poorly characterized. In contrast, vertebrate organisms, express two paralogous GCN5-like acetyltransferases (GCN5 and PCAF), which have been found so far only in SAGA-type complexes referred to hereafter as the STAGA (SPT3-TAF9-GCN5/PCAF acetylase) complexes. We now report the purification and characterization of vertebrate (human) ATAC-type complexes and identify novel components of STAGA. We show that human ATAC complexes incorporate in addition to GCN5 or PCAF (GCN5/PCAF), other epigenetic coregulators (ADA2-A, ADA3, STAF36, and WDR5), cofactors of chromatin assembly/remodeling and DNA replication machineries (POLE3/CHRAC17 and POLE4), the stress- and TGFbeta-activated protein kinase (TAK1/MAP3K7) and MAP3-kinase regulator (MBIP), additional cofactors of unknown function, and a novel YEATS2-NC2beta histone fold module that interacts with the TATA-binding protein (TBP) and negatively regulates transcription when recruited to a promoter. We further identify the p38 kinase-interacting protein (p38IP/FAM48A) as a novel component of STAGA with distant similarity to yeast Spt20. These results suggest that vertebrate ATAC-type and STAGA-type complexes link specific extracellular signals to modification of chromatin structure and regulation of the basal transcription machinery.
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Text Mining Data
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Manually curated Databases
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IRef Biogrid Interaction:
WDR5
—
TADA2A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DRAP1
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA3
—
SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2B
—
SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
SUPT3H
(colocalization, biochemical)
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IRef Biogrid Interaction:
CCDC101
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
UBAP2L
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DR1
—
SUPT20H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
HCFC1
—
YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DR1
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
POLE4
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YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCDC101
—
SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2B
—
SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
CSRP2BP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
ZZZ3
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YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
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POLE3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
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KAT2B
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
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DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2B
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2B
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
—
KAT2A
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2A
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DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCDC101
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MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCDC101
—
MAP3K7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2A
—
YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2B
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCDC101
—
YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
MAP3K7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2B
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA2A
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
YEATS2
(colocalization, biochemical)
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IRef Biogrid Interaction:
KAT2A
—
YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DR1
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
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KAT2B
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
—
TADA3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
TADA3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA3
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
DR1
(colocalization, biochemical)
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IRef Biogrid Interaction:
KAT2A
—
DR1
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
YEATS2
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TADA3
—
MAP3K7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DR1
—
MAP3K7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
—
YEATS2
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
CCDC101
—
WDR5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
DR1
—
TADA3
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
MAP3K7
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
DRAP1
(colocalization, biochemical)
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IRef Biogrid Interaction:
WDR5
—
WDR5
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
KAT2A
—
KAT2A
(colocalization, biochemical)
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IRef Biogrid Interaction:
TRRAP
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SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MBIP
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
WDR5
—
MBIP
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
TAF12
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SUPT3H
(physical association, affinity chromatography technology)
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IRef Biogrid Interaction:
MAP3K7
—
MAP3K7
(physical association, affinity chromatography technology)
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Gene Ontology Complexes SAGA-type complex:
SAGA-type complex complex (TADA2B-TADA1-USP22)
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Gene Ontology Complexes Ada2/Gcn5/Ada3 transcription activator complex:
Ada2/Gcn5/Ada3 transcription activator complex complex (WDR5-ZZZ3-UBAP2L-DR1-KAT2B-KAT2A-YEATS2-POLE4-POLE3-TADA3-CSRP2BP)
In total, 95 gene pairs are associated to this article in curated databases