J Cell Sci 2008,
PMID: 18187454
Fournier, Alaina K; Campbell, Latoya E; Castagnino, Paola; Liu, Wendy F; Chung, Betty M; Weaver, Valerie M; Chen, Christopher S; Assoian, Richard K
Integrin-mediated adhesion to substratum is required for cyclin D1 induction in mesenchymal cells, but we show here that the induction of cyclin D1 persists despite blockade of ECM-integrin signaling in MCF10A mammary epithelial cells. E-cadherin-mediated cell-cell adhesion also supports cyclin D1 induction in these cells, and the combined inhibition of both E-cadherin and integrin adhesion is required to prevent the expression of cyclin D1 mRNA and protein. Our previous studies described a pro-proliferative effect of E-cadherin in MCF10A cells, mediated by Rac, and we now show that Rac is required for cyclin D1 mRNA induction by both E-cadherin and integrin engagement. The levels of p21Cip1 and p27Kip1, Cdk inhibitors that are also targets of integrin signaling, are not affected by E-cadherin-mediated cell-cell adhesion. Finally, we show that the increased expression of cyclin D1 mRNA associated with E-cadherin-dependent cell-cell adhesion is causally linked to an increased entry into S phase. Our results identify Rac signaling to cyclin D1 as a crucial pro-proliferative effect of E-cadherin-mediated cell-cell adhesion.
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Text Mining Data
cyclin D1 → Rac: "
Rac dependent
cyclin D1 gene expression regulated by cadherin- and integrin mediated adhesion
"
cyclin D1 ⊣ E-cadherin: "
E-cadherin mediated cell-cell adhesion also supports cyclin D1 induction in these cells, and the combined inhibition of both E-cadherin and integrin adhesion is required to prevent the expression of cyclin D1 mRNA and protein
"
cyclin D1 → E-cadherin: "
Our previous studies described a pro-proliferative effect of E-cadherin in MCF10A cells, mediated by Rac, and we now show that Rac is required for cyclin D1 mRNA induction by both E-cadherin and integrin engagement
"
cyclin D1 → Rac: "
Our previous studies described a pro-proliferative effect of E-cadherin in MCF10A cells, mediated by Rac, and we now show that Rac is required for cyclin D1 mRNA induction by both E-cadherin and integrin engagement
"
Cdk ⊣ p21Cip1: "
The levels of p21Cip1 and p27Kip1, Cdk inhibitors that are also targets of integrin signaling, are not affected by E-cadherin mediated cell-cell adhesion
"
Cdk ⊣ p27Kip1: "
The levels of p21Cip1 and p27Kip1 , Cdk inhibitors that are also targets of integrin signaling, are not affected by E-cadherin mediated cell-cell adhesion
"
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