Gene interactions and pathways from curated databases and text-mining
J Dent Res 2007, PMID: 17720860

VIP inhibits P. gingivalis LPS-induced IL-18 and IL-18BPa in monocytes.

Foster, N; Andreadou, K; Jamieson, L; Preshaw, P M; Taylor, J J

IL-18 is a pro-inflammatory cytokine that is important in the regulation of T-cells and is elevated in inflammatory disorders such as periodontal disease. Vasoactive intestinal peptide (VIP) modulates immune responses to the periodontal pathogen Porphyromonas gingivalis (Pg). Our objective was to investigate the effect of Pg LPS on IL-18 and its natural inhibitor, IL-18 binding protein (IL-18BPa), in human monocytes, and the effect of VIP on this system. We demonstrated that Pg LPS induced both IL-18 and IL-18BPa secretion in cultures of the human monocytic cell line THP-1, as measured by specific ELISA. The addition of antibodies to IL-18BPa to the stimulated THP-1 cultures resulted in increased levels of free IL-18, indicating a specific interaction between IL18 and IL-18BPa in this system. VIP (10(-8)M) inhibited both IL-18 and IL-18Bpa secretion by stimulated monocytes. We conclude that IL-18 and IL-18BPa secretion by monocytes is part of the immune response to Pg, and that VIP can inhibit this process.

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Text Mining Data

IL-18BPa ⊣ VIP: " VIP inhibits P. gingivalis LPS induced IL-18 and IL-18BPa in monocytes "

IL-18 ⊣ VIP: " VIP inhibits P. gingivalis LPS induced IL-18 and IL-18BPa in monocytes "

IL-18BPa → LPS: " VIP inhibits P. gingivalis LPS induced IL-18 and IL-18BPa in monocytes "

IL-18 → LPS: " VIP inhibits P. gingivalis LPS induced IL-18 and IL-18BPa in monocytes "

IL-18 — VIP: " Our objective was to investigate the effect of Pg LPS on IL-18 and its natural inhibitor, IL-18 binding protein ( IL-18BPa ), in human monocytes, and the effect of VIP on this system "

IL-18 — LPS: " Our objective was to investigate the effect of Pg LPS on IL-18 and its natural inhibitor, IL-18 binding protein ( IL-18BPa ), in human monocytes, and the effect of VIP on this system "

IL-18BPa → LPS: " We demonstrated that Pg LPS induced both IL-18 and IL-18BPa secretion in cultures of the human monocytic cell line THP-1, as measured by specific ELISA "

IL-18 → LPS: " We demonstrated that Pg LPS induced both IL-18 and IL-18BPa secretion in cultures of the human monocytic cell line THP-1, as measured by specific ELISA "

IL-18Bpa ⊣ VIP: " VIP ( 10 ( -8 ) M ) inhibited both IL-18 and IL-18Bpa secretion by stimulated monocytes "

IL-18 ⊣ VIP: " VIP ( 10 ( -8 ) M ) inhibited both IL-18 and IL-18Bpa secretion by stimulated monocytes "

Manually curated Databases

No curated data.