Gene interactions and pathways from curated databases and text-mining
Cell cycle (Georgetown, Tex.) 2007, PMID: 17426454

NFkappaB-independent signaling to the cyclin D1 gene by Rac.

Klein, Eric A; Yang, Chengfeng; Kazanietz, Marcelo G; Assoian, Richard K

In this report we characterize the mechanism of Rac-mediated cyclin D1 gene expression in mouse embryonic fibroblasts. Activated Rac strongly stimulated cyclin D1 gene transcription but did not alter the half-life of cyclin D1 mRNA. Inhibition of NFkappaB signaling with the IkappaB super-repressor blocked the Rac-dependent expression of cyclin D1 mRNA, and this effect was selective since ERK-dependent cyclin D1 mRNA induction was minimally affected by super-repressor expression. However, we found that p65 activity in this system was induced by serum and not by activated Rac. Moreover, mouse cyclin D1 promoter-luciferase assays showed that Rac stimulated cyclin D1 gene expression without activating NFkappaB and that an essential Rac-regulated promoter element is located far upstream or downstream of the cyclin D1 transcription start site. We conclude that, in MEFs, Rac-mediated induction of cyclin D1 mRNA requires activation of a parallel NFkappaB pathway whereas ERK induces cyclin D1 transcription independent of NFkappaB.

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Text Mining Data

cyclin D1 → Rac: " In this report we characterize the mechanism of Rac mediated cyclin D1 gene expression in mouse embryonic fibroblasts "

cyclin D1 → Rac: " Activated Rac strongly stimulated cyclin D1 gene transcription but did not alter the half-life of cyclin D1 mRNA "

cyclin D1 → ERK: " Inhibition of NFkappaB signaling with the IkappaB super-repressor blocked the Rac dependent expression of cyclin D1 mRNA, and this effect was selective since ERK dependent cyclin D1 mRNA induction was minimally affected by super-repressor expression "

p65 → Rac: " However, we found that p65 activity in this system was induced by serum and not by activated Rac "

cyclin D1 → Rac: " Moreover, mouse cyclin D1 promoter-luciferase assays showed that Rac stimulated cyclin D1 gene expression without activating NFkappaB and that an essential Rac regulated promoter element is located far upstream or downstream of the cyclin D1 transcription start site "

cyclin D1 → ERK: " We conclude that, in MEFs, Rac mediated induction of cyclin D1 mRNA requires activation of a parallel NFkappaB pathway whereas ERK induces cyclin D1 transcription independent of NFkappaB "

Manually curated Databases

No curated data.