Gene interactions and pathways from curated databases and text-mining
J Immunol 2007, PMID: 17202341

IL-10 regulation by HIV-Tat in primary human monocytic cells: involvement of calmodulin/calmodulin-dependent protein kinase-activated p38 MAPK and Sp-1 and CREB-1 transcription factors.

Gee, Katrina; Angel, Jonathan B; Mishra, Sasmita; Blahoianu, Maria A; Kumar, Ashok

The anti-inflammatory cytokine, IL-10 plays an important role in HIV immunopathogenesis. The HIV accessory protein, Tat is not only critical for viral replication, but affects the host immune system by influencing cytokine production including IL-10. During HIV infection, IL-10 production by monocytic cells is up-regulated, representing a critical pathway by which HIV may induce immunodeficiency. Herein, we show that extracellular Tat-induced IL-10 expression in normal human monocytes. To understand the signaling pathways underlying HIV-Tat induced IL-10 transcription, we investigated the involvement of MAPK as well as calcium signaling and the downstream transcription factor(s). Our results suggest that Tat-induced calcium influx regulated IL-10 transcription in monocytic cells. The experiments designed to further understand the molecules involved in the calcium signaling suggested that calmodulin and calmodulin-dependent protein kinase-II (CaMK-II)-activated p38 MAPK played a role in extracellular Tat-induced IL-10 expression in primary human monocytes. Furthermore, Tat-induced IL-10 expression was regulated by p38 MAPK- and CaMK II-activated CREB-1 as well as Sp-1 transcription factors. Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II-dependent p38 MAPK.

Document information provided by NCBI PubMed

Text Mining Data

MAPK → HIV-Tat: " IL-10 regulation by HIV-Tat in primary human monocytic cells : involvement of calmodulin/calmodulin dependent protein kinase activated p38 MAPK and Sp-1 and CREB-1 transcription factors "

MAPK → IL-10: " IL-10 regulation by HIV-Tat in primary human monocytic cells : involvement of calmodulin/calmodulin dependent protein kinase activated p38 MAPK and Sp-1 and CREB-1 transcription factors "

IL-10 — HIV-Tat: " IL-10 regulation by HIV-Tat in primary human monocytic cells : involvement of calmodulin/calmodulin dependent protein kinase activated p38 MAPK and Sp-1 and CREB-1 transcription factors "

IL-10 → Tat: " Herein, we show that extracellular Tat induced IL-10 expression in normal human monocytes "

IL-10 → Tat: " The experiments designed to further understand the molecules involved in the calcium signaling suggested that calmodulin and calmodulin dependent protein kinase-II ( CaMK-II ) -activated p38 MAPK played a role in extracellular Tat induced IL-10 expression in primary human monocytes "

IL-10 — calmodulin: " The experiments designed to further understand the molecules involved in the calcium signaling suggested that calmodulin and calmodulin dependent protein kinase-II ( CaMK-II ) -activated p38 MAPK played a role in extracellular Tat induced IL-10 expression in primary human monocytes "

IL-10 → Tat: " Furthermore, Tat induced IL-10 expression was regulated by p38 MAPK- and CaMK II-activated CREB-1 as well as Sp-1 transcription factors "

IL-10 → CREB-1: " Furthermore, Tat induced IL-10 expression was regulated by p38 MAPK- and CaMK II-activated CREB-1 as well as Sp-1 transcription factors "

IL-10 → MAPK-: " Furthermore, Tat induced IL-10 expression was regulated by p38 MAPK- and CaMK II-activated CREB-1 as well as Sp-1 transcription factors "

p38 → calmodulin/CaMK-II: " Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II dependent p38 MAPK "

p38 → calmodulin/CaMK-II: " Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II dependent p38 MAPK "

IL-10 → Sp-1: " Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II dependent p38 MAPK "

IL-10 → CREB-1: " Taken together, our results suggest that extracellular HIV-Tat induced IL-10 transcription in primary human monocytes is regulated by CREB-1 and Sp-1 transcription factors through the activation of calmodulin/CaMK-II dependent p38 MAPK "

Manually curated Databases

No curated data.