J Biochem 2007,
PMID: 17202194
Nakanishi, Osamu; Suetsugu, Shiro; Yamazaki, Daisuke; Takenawa, Tadaomi
Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2 and WAVE3. Among them, WAVE2 is crucial for activation of the Arp2/3 complex for the formation of branched actin filaments in lamellipodia. Activation of mitogen-activated protein (MAP) kinase signalling results in the phosphorylation of the WAVE family proteins; however, which of the three WAVE proteins is phosphorylated is unclear. We found that in vitro WAVE2 is directly phosphorylated by a MAP kinase, i.e. extracellular signal-regulated kinase (ERK) 2. The proline-rich region and the verprolin, cofilin and acidic (VCA) region of WAVE2 were phosphorylated. Interestingly, the phosphorylated VCA region had a higher affinity for the Arp2/3 complex. However, the phosphorylation of the VCA region resulted in reduced induction of Arp2/3-mediated actin polymerization in vitro. The role of the phosphorylation of the proline-rich region was not determined.
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Text Mining Data
Arp2/3 complex — WAVE2: "
Effect of
WAVE2 phosphorylation on activation of the
Arp2/3 complex
"
Manually curated Databases
-
Reactome Reaction:
BRK1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
MAPK3
→
WASF2
(reaction)
-
Reactome Reaction:
MAPK1
→
NCKAP1
(reaction)
-
Reactome Reaction:
RAC1
→
CYFIP2
(indirect_complex)
-
Reactome Reaction:
NCKAP1
→
RAC1
(indirect_complex)
-
Reactome Reaction:
WASF2
→
RAC1
(indirect_complex)
-
Reactome Reaction:
CYFIP2
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
MAPK1
→
BAIAP2
(reaction)
-
Reactome Reaction:
MAPK1
→
WASF2
(reaction)
-
Reactome Reaction:
MAPK1
→
CYFIP2
(reaction)
-
Reactome Reaction:
MAPK3
→
CYFIP2
(reaction)
-
Reactome Reaction:
MAPK1
→
ABI1
(reaction)
-
Reactome Reaction:
NCKAP1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
MAPK1
→
BRK1
(reaction)
-
Reactome Reaction:
MAPK3
→
ABI1
(reaction)
-
Reactome Reaction:
MAPK1
→
RAC1
(reaction)
-
Reactome Reaction:
MAPK3
→
NCKAP1
(reaction)
-
Reactome Reaction:
RAC1
→
ABI1
(indirect_complex)
-
Reactome Reaction:
MAPK3
→
BAIAP2
(reaction)
-
Reactome Reaction:
MAPK3
→
RAC1
(reaction)
-
Reactome Reaction:
RAC1
→
BRK1
(indirect_complex)
-
Reactome Reaction:
WASF2
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
MAPK3
→
BRK1
(reaction)
-
Reactome Reaction:
RAC1
→
BAIAP2
(indirect_complex)
-
Reactome Reaction:
ABI1
→
BAIAP2
(indirect_complex)
In total, 25 gene pairs are associated to this article in curated databases