Gene interactions and pathways from curated databases and text-mining
J Biochem 2007, PMID: 17202194

Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex.

Nakanishi, Osamu; Suetsugu, Shiro; Yamazaki, Daisuke; Takenawa, Tadaomi

Members of the family of WASP-family Verprolin homologous proteins (WAVEs) activate the Arp2/3 complex to induce actin polymerization. The WAVE family comprises three proteins, namely, WAVE1, WAVE2 and WAVE3. Among them, WAVE2 is crucial for activation of the Arp2/3 complex for the formation of branched actin filaments in lamellipodia. Activation of mitogen-activated protein (MAP) kinase signalling results in the phosphorylation of the WAVE family proteins; however, which of the three WAVE proteins is phosphorylated is unclear. We found that in vitro WAVE2 is directly phosphorylated by a MAP kinase, i.e. extracellular signal-regulated kinase (ERK) 2. The proline-rich region and the verprolin, cofilin and acidic (VCA) region of WAVE2 were phosphorylated. Interestingly, the phosphorylated VCA region had a higher affinity for the Arp2/3 complex. However, the phosphorylation of the VCA region resulted in reduced induction of Arp2/3-mediated actin polymerization in vitro. The role of the phosphorylation of the proline-rich region was not determined.

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Text Mining Data

Arp2/3 complex — WAVE2: " Effect of WAVE2 phosphorylation on activation of the Arp2/3 complex "

Manually curated Databases

  • Reactome Reaction: BRK1 → BAIAP2 (indirect_complex)
  • Reactome Reaction: MAPK3 → WASF2 (reaction)
  • Reactome Reaction: MAPK1 → NCKAP1 (reaction)
  • Reactome Reaction: RAC1 → CYFIP2 (indirect_complex)
  • Reactome Reaction: NCKAP1 → RAC1 (indirect_complex)
  • Reactome Reaction: WASF2 → RAC1 (indirect_complex)
  • Reactome Reaction: CYFIP2 → BAIAP2 (indirect_complex)
  • Reactome Reaction: MAPK1 → BAIAP2 (reaction)
  • Reactome Reaction: MAPK1 → WASF2 (reaction)
  • Reactome Reaction: MAPK1 → CYFIP2 (reaction)
  • Reactome Reaction: MAPK3 → CYFIP2 (reaction)
  • Reactome Reaction: MAPK1 → ABI1 (reaction)
  • Reactome Reaction: NCKAP1 → BAIAP2 (indirect_complex)
  • Reactome Reaction: MAPK1 → BRK1 (reaction)
  • Reactome Reaction: MAPK3 → ABI1 (reaction)
  • Reactome Reaction: MAPK1 → RAC1 (reaction)
  • Reactome Reaction: MAPK3 → NCKAP1 (reaction)
  • Reactome Reaction: RAC1 → ABI1 (indirect_complex)
  • Reactome Reaction: MAPK3 → BAIAP2 (reaction)
  • Reactome Reaction: MAPK3 → RAC1 (reaction)
  • Reactome Reaction: RAC1 → BRK1 (indirect_complex)
  • Reactome Reaction: WASF2 → BAIAP2 (indirect_complex)
  • Reactome Reaction: MAPK3 → BRK1 (reaction)
  • Reactome Reaction: RAC1 → BAIAP2 (indirect_complex)
  • Reactome Reaction: ABI1 → BAIAP2 (indirect_complex)
In total, 25 gene pairs are associated to this article in curated databases