Gene interactions and pathways from curated databases and text-mining
J Immunol 2006, PMID: 16818775

Granzyme M directly cleaves inhibitor of caspase-activated DNase (CAD) to unleash CAD leading to DNA fragmentation.

Lu, Hongxia; Hou, Qiang; Zhao, Tongbiao; Zhang, Honglian; Zhang, Qixiang; Wu, Lianfeng; Fan, Zusen

Granzyme (Gzm)M is constitutively highly expressed in NK cells that may play a critical role in NK cell-mediated cytolysis. However, the function of GzmM has been less defined. Just one report showed GzmM induces a caspase-independent death pathway. In this study, we demonstrate a protein transfection reagent Pro-Ject can efficiently transport GzmM into target cells. GzmM initiates caspase-dependent apoptosis with typical apoptotic nuclear morphology. GzmM induces DNA fragmentation, not DNA nicking. GzmM can directly degrade inhibitor of caspase-activated DNase to release the nuclease activity of caspase-activated DNase for damaging DNA. Furthermore, GzmM cleaves the DNA damage sensor enzyme poly(ADP-ribose) polymerase to prevent cellular DNA repair and force apoptosis.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: GZMM — PARP1 (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: GZMM — DFFA (direct interaction, enzymatic study)
  • IRef Innatedb Interaction: GZMM — CASP3 (unknown, -)
  • IRef Innatedb Interaction: GZMM — DFFA (unknown, -)
In total, 3 gene pairs are associated to this article in curated databases