FASEB J 2006,
PMID: 16571778
Kang, Bit-Na; Tirumurugaan, K G; Deshpande, Deepak A; Amrani, Yassine; Panettieri, Reynold A; Walseth, Timothy F; Kannan, Mathur S
The transmembrane glycoprotein CD38 catalyzes the synthesis of the calcium mobilizing molecule cyclic ADP-ribose from NAD. In human airway smooth muscle (HASM) cells, the expression and function of CD38 are augmented by the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha), leading to increased intracellular calcium response to agonists. A glucocorticoid response element in the CD38 gene has been computationally described, providing evidence for transcriptional regulation of its expression. In the present study, we investigated the effects of dexamethasone, a glucocorticoid, on CD38 expression and ADP-ribosyl cyclase activity in HASM cells stimulated with TNF-alpha. In HASM cells, TNF-alpha augmented CD38 expression and ADP-ribosyl cyclase activity, which were attenuated by dexamethasone. TNF-alpha increased NF-kappaB expression and its activation, and dexamethasone partially reversed these effects. TNF-alpha increased the expression of IkappaBalpha, and dexamethasone increased it further. An inhibitor of NF-kappaB activation or transfection of cells with IkappaB mutants decreased TNF-alpha-induced CD38 expression. The results indicate that TNF-alpha-induced CD38 expression involves NF-kappaB expression and its activation and dexamethasone inhibits CD38 expression through NF-kappaB-dependent and -independent mechanisms.
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Text Mining Data
CD38 — tumor necrosis factor-alpha: "
Transcriptional
regulation of
CD38 expression by
tumor necrosis factor-alpha in human airway smooth muscle cells : role of NF-kappaB and sensitivity to glucocorticoids
"
CD38 → tumor necrosis factor-alpha (TNF-alpha): "
In human airway smooth muscle ( HASM ) cells, the expression and function of CD38 are augmented by the inflammatory cytokine tumor necrosis factor-alpha (TNF-alpha) , leading to increased intracellular calcium response to agonists
"
CD38 → TNF-alpha: "
In HASM cells, TNF-alpha augmented CD38 expression and ADP-ribosyl cyclase activity, which were attenuated by dexamethasone
"
NF-kappaB → TNF-alpha: "
TNF-alpha increased NF-kappaB expression and its activation, and dexamethasone partially reversed these effects
"
IkappaBalpha → TNF-alpha: "
TNF-alpha increased the expression of IkappaBalpha , and dexamethasone increased it further
"
CD38 → TNF-alpha: "
An inhibitor of NF-kappaB activation or transfection of cells with IkappaB mutants decreased TNF-alpha induced CD38 expression
"
CD38 → TNF-alpha: "
The results indicate that TNF-alpha induced CD38 expression involves NF-kappaB expression and its activation and dexamethasone inhibits CD38 expression through NF-kappaB dependent and -independent mechanisms
"
CD38 → NF-kappaB: "
The results indicate that TNF-alpha induced CD38 expression involves NF-kappaB expression and its activation and dexamethasone inhibits CD38 expression through NF-kappaB dependent and -independent mechanisms
"
Manually curated Databases
No curated data.