FEBS Lett 2006,
PMID: 16455083
Ligon, Lee A; Shelly, Spencer S; Tokito, Mariko K; Holzbaur, Erika L F
Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP-170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued, which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo.
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Text Mining Data
CLIP-170 ⊣ p150Glued: "
The interaction between
CLIP-170 and EB1 is competitively
inhibited by the related CAP-Gly protein
p150Glued , which also localizes to microtubule plus ends in vivo
"
EB1 ⊣ p150Glued: "
The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued , which also localizes to microtubule plus ends in vivo
"
Manually curated Databases
-
IRef Biogrid Interaction:
MAPRE1
—
TUBB
(direct interaction, pull down)
-
IRef Biogrid Interaction:
TUBA1A
—
CLIP1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
MAPRE1
—
TUBA1A
(direct interaction, pull down)
-
IRef Biogrid Interaction:
TUBB
—
CLIP1
(direct interaction, pull down)
-
IRef Biogrid Interaction:
MAPRE1
—
CLIP1
(direct interaction, pull down)
-
IRef Hprd Interaction:
CLIP1
—
MAPRE2
(in vitro)
-
IRef Intact Interaction:
TUBA4A
—
CLIP1
(direct interaction, affinity chromatography technology)
-
IRef Intact Interaction:
TUBA4A
—
CLIP1
(direct interaction, pull down)
-
IRef Intact Interaction:
TUBA4A
—
CLIP1
(direct interaction, saturation binding)
-
IRef Intact Interaction:
MAPRE1
—
CLIP1
(direct interaction, chromatography technology)
In total, 7 gene pairs are associated to this article in curated databases