Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2006, PMID: 16455083

Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes.

Ligon, Lee A; Shelly, Spencer S; Tokito, Mariko K; Holzbaur, Erika L F

Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP-170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued, which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo.

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Text Mining Data

CLIP-170 ⊣ p150Glued: " The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued , which also localizes to microtubule plus ends in vivo "

EB1 ⊣ p150Glued: " The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150Glued , which also localizes to microtubule plus ends in vivo "

Manually curated Databases

  • IRef Biogrid Interaction: MAPRE1 — TUBB (direct interaction, pull down)
  • IRef Biogrid Interaction: TUBA1A — CLIP1 (direct interaction, pull down)
  • IRef Biogrid Interaction: MAPRE1 — TUBA1A (direct interaction, pull down)
  • IRef Biogrid Interaction: TUBB — CLIP1 (direct interaction, pull down)
  • IRef Biogrid Interaction: MAPRE1 — CLIP1 (direct interaction, pull down)
  • IRef Hprd Interaction: CLIP1 — MAPRE2 (in vitro)
  • IRef Intact Interaction: TUBA4A — CLIP1 (direct interaction, affinity chromatography technology)
  • IRef Intact Interaction: TUBA4A — CLIP1 (direct interaction, pull down)
  • IRef Intact Interaction: TUBA4A — CLIP1 (direct interaction, saturation binding)
  • IRef Intact Interaction: MAPRE1 — CLIP1 (direct interaction, chromatography technology)
In total, 7 gene pairs are associated to this article in curated databases