Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2004, PMID: 15465815

Spred-1 negatively regulates interleukin-3-mediated ERK/mitogen-activated protein (MAP) kinase activation in hematopoietic cells.

Nonami, Atsushi; Kato, Reiko; Taniguchi, Koji; Yoshiga, Daigo; Taketomi, Takaharu; Fukuyama, Satoru; Harada, Mine; Sasaki, Atsuo; Yoshimura, Akihiko

Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein (MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity. We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived mast cells. To investigate the roles of Spred-1 in hematopoiesis, we expressed wild-type Spred-1 and a dominant negative form of Spred-1, DeltaC-Spred, in IL-3- and stem cell factor (SCF)-dependent cell lines as well as hematopoietic progenitor cells from mouse bone marrow by retrovirus gene transfer. In IL-3-dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1 resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3. In contrast, DeltaC-Spred augmented IL-3-induced cell proliferation and ERK activation. Wild-type Spred-1 inhibited colony formation of bone marrow cells in the presence of cytokines, whereas DeltaC-Spred-1 expression enhanced colony formation. Augmentation of ERK activation and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells. These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF-induced but also IL-3-induced ERK activation.

Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
Document information provided by NCBI PubMed

Text Mining Data

ERK/mitogen activated protein ( MAP ) kinase → Spred-1: " Spred-1 negatively regulates interleukin-3 mediated ERK/mitogen activated protein ( MAP ) kinase activation in hematopoietic cells "

ERK/mitogen activated protein ( MAP ) kinase → interleukin-3: " Spred-1 negatively regulates interleukin-3 mediated ERK/mitogen activated protein ( MAP ) kinase activation in hematopoietic cells "

ERK/mitogen → Spred-1: " Spred-1 negatively regulates interleukin-3 mediated ERK/mitogen activated protein ( MAP ) kinase activation in hematopoietic cells "

ERK/mitogen → interleukin-3: " Spred-1 negatively regulates interleukin-3 mediated ERK/mitogen activated protein ( MAP ) kinase activation in hematopoietic cells "

ERK ⊣ Spred-1: " In IL-3 dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1 resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3 "

ERK → IL-3: " In IL-3 dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1 resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3 "

ERK → SCF: " In IL-3 dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1 resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3 "

ERK → Spred: " In contrast, DeltaC Spred augmented IL-3 induced cell proliferation and ERK activation "

ERK → IL-3: " In contrast, DeltaC Spred augmented IL-3 induced cell proliferation and ERK activation "

ERK → IL-3: " Augmentation of ERK activation and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow derived mast cells "

ERK → IL-3: " These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF induced but also IL-3 induced ERK activation "

ERK → SCF: " These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF induced but also IL-3 induced ERK activation "

Spred-1 — ERK: " These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF induced but also IL-3 induced ERK activation "

Spred-1 — IL-3: " These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF induced but also IL-3 induced ERK activation "

Spred-1 — SCF: " These data suggest that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF induced but also IL-3 induced ERK activation "

Manually curated Databases

In total, 3 gene pairs are associated to this article in curated databases