Mol Biol Cell 2004,
PMID: 15254269
Lewis, Stephen M; Poon, Pak Phi; Singer, Richard A; Johnston, Gerald C; Spang, Anne
The small GTPase Arf and coatomer (COPI) are required for the generation of retrograde transport vesicles. Arf activity is regulated by guanine exchange factors (ArfGEF) and GTPase-activating proteins (ArfGAPs). The ArfGAPs Gcs1 and Glo3 provide essential overlapping function for retrograde vesicular transport from the Golgi to the endoplasmic reticulum. We have identified Glo3 as a component of COPI vesicles. Furthermore, we find that a mutant version of the Glo3 protein exerts a negative effect on retrograde transport, even in the presence of the ArfGAP Gcs1. Finally, we present evidence supporting a role for ArfGAP protein in the generation of COPI retrograde transport vesicles.
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Text Mining Data
Arf — ArfGEF: "
Arf activity is
regulated by guanine exchange factors (
ArfGEF ) and GTPase activating proteins ( ArfGAPs )
"
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