Gene interactions and pathways from curated databases and text-mining
Biochem Biophys Res Commun 2003, PMID: 12890486

Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1-induced MEK/ERK phosphorylation and migration in endothelial cells.

Yoon, Mi-Jeong; Cho, Chung-Hyun; Lee, Chang Sup; Jang, Il-Ho; Ryu, Sung Ho; Koh, Gou Young

Angiopoietin-1 (Ang1) and its receptor, Tie2, play critical roles in blood vessel formation. Ang1 triggers a variety of signaling events in endothelial cells leading to vasculogenic and angiogenic processes. However, the underlying mechanism for Ang1/Tie2 signaling is not fully understood. Here, we show that Tie2 and phospholipase D (PLD) are localized in the caveolae, specialized subdomains of the endothelial cell plasma membrane enriched with signaling molecules. Interestingly, Ang1 increased PLD activities in a dose- and time-dependent manner. Ang1-induced MEK/ERK activation was abrogated when PLD was inhibited, suggesting that PLD mediates Ang1-induced MEK/ERK activation. Moreover, PLD inhibitor, 1-butanol, inhibited Ang1-induced endothelial cell migration. Our results indicate that: (1) caveolae may be the platform for Tie2/PLD association in endothelial cells; (2) PLD is a new mediator of Ang1/Tie2-induced signaling pathway, and it participates in MAPK activation and endothelial cell migration.

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Text Mining Data

MEK/ERK → Tie2: " Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1 induced MEK/ERK phosphorylation and migration in endothelial cells "

MEK/ERK → phospholipase D: " Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1 induced MEK/ERK phosphorylation and migration in endothelial cells "

MEK/ERK → angiopoietin-1: " Localization of Tie2 and phospholipase D in endothelial caveolae is involved in angiopoietin-1 induced MEK/ERK phosphorylation and migration in endothelial cells "

PLD → Ang1: " Interestingly, Ang1 increased PLD activities in a dose- and time dependent manner "

MEK/ERK → PLD: " Ang1 induced MEK/ERK activation was abrogated when PLD was inhibited, suggesting that PLD mediates Ang1 induced MEK/ERK activation "

MEK/ERK → Ang1: " Ang1 induced MEK/ERK activation was abrogated when PLD was inhibited, suggesting that PLD mediates Ang1 induced MEK/ERK activation "

MAPK → Ang1/Tie2: " Our results indicate that : ( 1 ) caveolae may be the platform for Tie2/PLD association in endothelial cells ; ( 2 ) PLD is a new mediator of Ang1/Tie2 induced signaling pathway, and it participates in MAPK activation and endothelial cell migration "

MAPK → Ang1/Tie2: " Our results indicate that : ( 1 ) caveolae may be the platform for Tie2/PLD association in endothelial cells ; ( 2 ) PLD is a new mediator of Ang1/Tie2 induced signaling pathway, and it participates in MAPK activation and endothelial cell migration "

Manually curated Databases

In total, 8 gene pairs are associated to this article in curated databases