Eur J Immunol 2003,
PMID: 12672049
Nishioka, Hiroaki; Baesso, Ilenia; Semenzato, Gianpietro; Trentin, Livio; Rappuoli, Rino; Del Giudice, Giuseppe; Montecucco, Cesare
Infection by Helicobacter pylori causes an acute inflammatory response followed by a chronic infection of the human gastric mucosa characterized by the infiltration of neutrophils andmononuclear inflammatory cells. The neutrophil-activating protein of Helicobacter pylori (HP-NAP) is a virulence factor that activates neutrophils, monocytes, and mast cells. However, the mechanism by which HP-NAP activates these cells is not fully understood. Here, we show that HP-NAP induces extracellular regulated kinase (ERK) and p38-mitogen-activated protein kinase (MAPK) activation in human neutrophils; c-Jun N-terminal kinase is not activated by HP-NAP. A MAPK/ERK kinase inhibitor and a p38-MAPK inhibitor suppress HP-NAP-mediated neutrophil oxidative burst, adhesion, andchemotaxis, but not actin polymerization. Pertussis toxin (PTX) inhibits all these neutrophil functions and the MAPK activation caused by HP-NAP. These results demonstrate that HP-NAP activates neutrophils through a PTX-sensitive pathway and that ERK and p38-MAPK are involved in many neutrophil functions stimulated by HP-NAP.
Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
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Text Mining Data
p38-mitogen activated protein kinase → HP-NAP: "
Here, we show that
HP-NAP induces extracellular regulated kinase (ERK) and
p38-mitogen activated protein kinase ( MAPK ) activation in human neutrophils ; c-Jun N-terminal kinase is not activated by HP-NAP
"
extracellular regulated kinase (ERK) → HP-NAP: "
Here, we show that HP-NAP induces extracellular regulated kinase (ERK) and p38-mitogen activated protein kinase ( MAPK ) activation in human neutrophils ; c-Jun N-terminal kinase is not activated by HP-NAP
"
p38-mitogen → HP-NAP: "
Here, we show that HP-NAP induces extracellular regulated kinase (ERK) and p38-mitogen activated protein kinase ( MAPK ) activation in human neutrophils ; c-Jun N-terminal kinase is not activated by HP-NAP
"
MAPK ⊣ Pertussis toxin ( PTX ): "
Pertussis toxin ( PTX ) inhibits all these neutrophil functions and the MAPK activation caused by HP-NAP
"
HP-NAP → p38-MAPK: "
These results demonstrate that HP-NAP activates neutrophils through a PTX-sensitive pathway and that ERK and p38-MAPK are involved in many neutrophil functions stimulated by HP-NAP
"
HP-NAP → ERK: "
These results demonstrate that HP-NAP activates neutrophils through a PTX-sensitive pathway and that ERK and p38-MAPK are involved in many neutrophil functions stimulated by HP-NAP
"
HP-NAP → p38-MAPK: "
These results demonstrate that HP-NAP activates neutrophils through a PTX-sensitive pathway and that ERK and p38-MAPK are involved in many neutrophil functions stimulated by HP-NAP
"
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