Mouse Gene Pak1 (ENSMUST00000033040.11) from GENCODE VM23 Comprehensive Transcript Set (only Basic displayed by default)
  Description: Mus musculus p21 (RAC1) activated kinase 1 (Pak1), transcript variant 1, mRNA. (from RefSeq NM_011035)
Gencode Transcript: ENSMUST00000033040.11
Gencode Gene: ENSMUSG00000030774.13
Transcript (Including UTRs)
   Position: mm10 chr7:97,842,935-97,912,381 Size: 69,447 Total Exon Count: 15 Strand: +
Coding Region
   Position: mm10 chr7:97,854,460-97,911,182 Size: 56,723 Coding Exon Count: 14 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDRNA Structure
Protein StructureOther SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther Names
Model InformationMethods
Data last updated at UCSC: 2019-09-19

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr7:97,842,935-97,912,381)mRNA (may differ from genome)Protein (544 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsMGI
PubMedReactomeSOURCEUniProtKBWikipedia

-  Comments and Description Text from UniProtKB
  ID: G5E884_MOUSE
DESCRIPTION: SubName: Full=Serine/threonine-protein kinase PAK 1; SubName: Full=p21 (CDKN1A)-activated kinase 1, isoform CRA_b;
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SIMILARITY: Contains 1 CRIB domain.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -110.30231-0.477 Picture PostScript Text
3' UTR -334.901199-0.279 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000095 - PAK_box_Rho-bd
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR002290 - Ser/Thr_dual-sp_kinase_dom
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF00786 - P21-Rho-binding domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
47912 - Wiscott-Aldrich syndrome protein, WASP, C-terminal domain
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on G5E884
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserNo orthologNo orthologGenome BrowserNo ortholog
Gene Details    Gene Details
Gene Sorter    Gene Sorter
 RGDEnsembl WormBaseSGD
 Protein Sequence  Protein Sequence 
 Alignment  Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005518 collagen binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019901 protein kinase binding
GO:0048365 Rac GTPase binding

Biological Process:
GO:0001666 response to hypoxia
GO:0001934 positive regulation of protein phosphorylation
GO:0006468 protein phosphorylation
GO:0008284 positive regulation of cell proliferation
GO:0010033 response to organic substance
GO:0010763 positive regulation of fibroblast migration
GO:0016310 phosphorylation
GO:0021549 cerebellum development
GO:0030010 establishment of cell polarity
GO:0030335 positive regulation of cell migration
GO:0031116 positive regulation of microtubule polymerization
GO:0031532 actin cytoskeleton reorganization
GO:0032869 cellular response to insulin stimulus
GO:0032956 regulation of actin cytoskeleton organization
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0033148 positive regulation of intracellular estrogen receptor signaling pathway
GO:0042060 wound healing
GO:0043507 positive regulation of JUN kinase activity
GO:0045773 positive regulation of axon extension
GO:0046777 protein autophosphorylation
GO:0048012 hepatocyte growth factor receptor signaling pathway
GO:0048754 branching morphogenesis of an epithelial tube
GO:0048812 neuron projection morphogenesis
GO:0051496 positive regulation of stress fiber assembly
GO:0060244 negative regulation of cell proliferation involved in contact inhibition
GO:0061052 negative regulation of cell growth involved in cardiac muscle cell development
GO:0090314 positive regulation of protein targeting to membrane
GO:1904707 positive regulation of vascular smooth muscle cell proliferation
GO:1904754 positive regulation of vascular associated smooth muscle cell migration
GO:2001275 positive regulation of glucose import in response to insulin stimulus

Cellular Component:
GO:0001726 ruffle
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005886 plasma membrane
GO:0005911 cell-cell junction
GO:0014704 intercalated disc
GO:0016020 membrane
GO:0030018 Z disc
GO:0030027 lamellipodium
GO:0030424 axon
GO:0030425 dendrite
GO:0031965 nuclear membrane
GO:0032991 macromolecular complex
GO:0071437 invadopodium


-  Descriptions from all associated GenBank mRNAs
  LF196446 - JP 2014500723-A/3949: Polycomb-Associated Non-Coding RNAs.
BC055740 - Mus musculus p21 (CDKN1A)-activated kinase 1, mRNA (cDNA clone IMAGE:6810707), containing frame-shift errors.
BC060157 - Mus musculus p21 (CDKN1A)-activated kinase 1, mRNA (cDNA clone IMAGE:6810707).
LF204629 - JP 2014500723-A/12132: Polycomb-Associated Non-Coding RNAs.
BC079838 - Mus musculus cDNA clone IMAGE:6812253, containing frame-shift errors.
AF082077 - Mus musculus Cdc42/Rac effector kinase PAK-A (Paka) mRNA, complete cds.
AK077497 - Mus musculus 8 days embryo whole body cDNA, RIKEN full-length enriched library, clone:5730422C08 product:p21 (CDKN1A)-activated kinase 1, full insert sequence.
BC145698 - Mus musculus cDNA clone IMAGE:40131001.
BC167167 - Synthetic construct Mus musculus clone IMAGE:100068067, MGC:195684 p21 (CDKN1A)-activated kinase 1 (Pak1) mRNA, encodes complete protein.
MA432023 - JP 2018138019-A/3949: Polycomb-Associated Non-Coding RNAs.
MA440206 - JP 2018138019-A/12132: Polycomb-Associated Non-Coding RNAs.
AK078711 - Mus musculus adult male eyeball cDNA, RIKEN full-length enriched library, clone:7530419B14 product:unclassifiable, full insert sequence.
LF294396 - JP 2014500723-A/101899: Polycomb-Associated Non-Coding RNAs.
LF294397 - JP 2014500723-A/101900: Polycomb-Associated Non-Coding RNAs.
LF294401 - JP 2014500723-A/101904: Polycomb-Associated Non-Coding RNAs.
LF294408 - JP 2014500723-A/101911: Polycomb-Associated Non-Coding RNAs.
LF294416 - JP 2014500723-A/101919: Polycomb-Associated Non-Coding RNAs.
MA529973 - JP 2018138019-A/101899: Polycomb-Associated Non-Coding RNAs.
MA529974 - JP 2018138019-A/101900: Polycomb-Associated Non-Coding RNAs.
MA529978 - JP 2018138019-A/101904: Polycomb-Associated Non-Coding RNAs.
MA529985 - JP 2018138019-A/101911: Polycomb-Associated Non-Coding RNAs.
MA529993 - JP 2018138019-A/101919: Polycomb-Associated Non-Coding RNAs.
LF294418 - JP 2014500723-A/101921: Polycomb-Associated Non-Coding RNAs.
MA529995 - JP 2018138019-A/101921: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
mmu04010 - MAPK signaling pathway
mmu04012 - ErbB signaling pathway
mmu04062 - Chemokine signaling pathway
mmu04360 - Axon guidance
mmu04510 - Focal adhesion
mmu04650 - Natural killer cell mediated cytotoxicity
mmu04660 - T cell receptor signaling pathway
mmu04666 - Fc gamma R-mediated phagocytosis
mmu04810 - Regulation of actin cytoskeleton
mmu05211 - Renal cell carcinoma

BioCarta from NCI Cancer Genome Anatomy Project
m_akapCentrosomePathway - Protein Kinase A at the Centrosome

Reactome (by CSHL, EBI, and GO)

Protein G5E884 (Reactome details) participates in the following event(s):

R-MMU-445072 Interaction of PAK1 with Rac1-GTP
R-MMU-2029456 RAC1 and CDC42 activate PAK1
R-MMU-5669240 FLNA binds PAK1
R-MMU-2029454 Autophosphorylation of PAK1
R-MMU-5669250 PAK1 phosphorylates FLNA
R-MMU-376123 DSCAM associates with Rac1-GTP:pPAK1
R-MMU-3928619 PAK1 binds RAC1:GTP
R-MMU-5669158 NF2 binds PAK1
R-MMU-430183 NCK binds PAK
R-MMU-389788 Activation of PAKs by RAC1 and CDC42
R-MMU-399930 Activation of PAK by Rac1
R-MMU-3928641 PAK binds RAC and bPIX
R-MMU-5357472 PAK1-3 autophosphorylates
R-MMU-5627775 Autophosphorylation of PAK1,2,3
R-MMU-2730856 Autophosphorylation of PAK
R-MMU-2029460 PAK1 phosphorylates LIMK1
R-MMU-5668947 PAK1 phosphorylates myosin phosphatase
R-MMU-3928620 PAK1 autophosphorylates
R-MMU-5357483 RAC1 binds PAK1-3
R-MMU-2730889 Recruitment of PAK to the membrane by binding active RAC1
R-MMU-399939 Autophosphorylation of PAK
R-MMU-5357445 PAK1-3 dimer disassociates
R-MMU-5624492 PAK phosphorylates p21 RAF1 on S338
R-MMU-3928625 PAKs autophosphorylate
R-MMU-5357477 PAK1-3 phosphorylates VE-cadherin
R-MMU-5687086 PAK1,2,3 phosphorylates MAPK6,4
R-MMU-5668984 PAK1 or PAK2 phosphorylates MYLK
R-MMU-451347 Activation of JNK by DSCAM
R-MMU-399952 Phosphorylation of LIMK-1 by PAK
R-MMU-3928640 PAKs phosphorylate MLC
R-MMU-445144 Signal transduction by L1
R-MMU-2029482 Regulation of actin dynamics for phagocytic cup formation
R-MMU-5627123 RHO GTPases activate PAKs
R-MMU-376172 DSCAM interactions
R-MMU-3928662 EPHB-mediated forward signaling
R-MMU-202433 Generation of second messenger molecules
R-MMU-389359 CD28 dependent Vav1 pathway
R-MMU-5687128 MAPK6/MAPK4 signaling
R-MMU-399954 Sema3A PAK dependent Axon repulsion
R-MMU-3928664 Ephrin signaling
R-MMU-373760 L1CAM interactions
R-MMU-2029480 Fcgamma receptor (FCGR) dependent phagocytosis
R-MMU-195258 RHO GTPase Effectors
R-MMU-5218920 VEGFR2 mediated vascular permeability
R-MMU-2871796 FCERI mediated MAPK activation
R-MMU-373752 Netrin-1 signaling
R-MMU-2682334 EPH-Ephrin signaling
R-MMU-202403 TCR signaling
R-MMU-389356 CD28 co-stimulation
R-MMU-5683057 MAPK family signaling cascades
R-MMU-373755 Semaphorin interactions
R-MMU-5621575 CD209 (DC-SIGN) signaling
R-MMU-422475 Axon guidance
R-MMU-168249 Innate Immune System
R-MMU-194315 Signaling by Rho GTPases
R-MMU-4420097 VEGFA-VEGFR2 Pathway
R-MMU-2454202 Fc epsilon receptor (FCERI) signaling
R-MMU-1280218 Adaptive Immune System
R-MMU-388841 Costimulation by the CD28 family
R-MMU-162582 Signal Transduction
R-MMU-5621481 C-type lectin receptors (CLRs)
R-MMU-1266738 Developmental Biology
R-MMU-168256 Immune System
R-MMU-194138 Signaling by VEGF
R-MMU-9006934 Signaling by Receptor Tyrosine Kinases

-  Other Names for This Gene
  Alternate Gene Symbols: G5E884, G5E884_MOUSE, mCG_7049, NM_011035, uc009ijv.1, uc009ijv.2
UCSC ID: uc009ijv.2
RefSeq Accession: NM_011035
Protein: G5E884 CCDS: CCDS21464.1

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.