ID:NOS1_MOUSE DESCRIPTION: RecName: Full=Nitric oxide synthase, brain; EC=1.14.13.39; AltName: Full=Constitutive NOS; AltName: Full=NC-NOS; AltName: Full=NOS type I; AltName: Full=Neuronal NOS; Short=N-NOS; Short=nNOS; AltName: Full=Peptidyl-cysteine S-nitrosylase NOS1; AltName: Full=bNOS; FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR. Isoform NNOS Mu may be an effector enzyme for the dystrophin complex. CATALYTIC ACTIVITY: 2 L-arginine + 3 NADPH + 4 O(2) = 2 L- citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O. COFACTOR: Heme group (By similarity). COFACTOR: Binds 1 FAD (By similarity). COFACTOR: Binds 1 FMN (By similarity). COFACTOR: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity). ENZYME REGULATION: Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP (By similarity). SUBUNIT: Homodimer. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Interacts with HTR4. Forms a ternary complex with CAPON and RASD1. Interacts with VAC14 (By similarity). Interacts with SLC6A4. SUBCELLULAR LOCATION: Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine (By similarity). Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). TISSUE SPECIFICITY: Widely expressed in the nervous system: expressed in cerebrum, olfactory bulb, hippocampus, midbrain, cerebellum, pons, medulla oblongata, and spinal cord. Also found in skeletal muscle, where it is localized beneath the sarcolemma of fast twitch muscle fibers, and in spleen, heart, kidney, and liver. N-NOS-1 and N-NOS-2 are found in all parts of the nervous system. NNOS beta and gamma occur in a region-specific manner in the brain and NNOS beta expression is developmentally regulated. NNOS Mu is only found in mature skeletal and cardiac muscles. INDUCTION: By cholinergic agonists acting at inositol phosphate- linked muscarinic receptors in cardiac myocytes. DOMAIN: The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity). PTM: Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro) (By similarity). DISEASE: Note=In MDX mice (mouse model of dystrophinopathy) the dystrophin complex is disrupted and nNOS is displaced from sarcolemma and accumulates in the cytosol. SIMILARITY: Belongs to the NOS family. SIMILARITY: Contains 1 FAD-binding FR-type domain. SIMILARITY: Contains 1 flavodoxin-like domain. SIMILARITY: Contains 1 PDZ (DHR) domain.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9Z0J4
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
Biological Process: GO:0001666 response to hypoxia GO:0002028 regulation of sodium ion transport GO:0006527 arginine catabolic process GO:0006809 nitric oxide biosynthetic process GO:0006941 striated muscle contraction GO:0007263 nitric oxide mediated signal transduction GO:0008016 regulation of heart contraction GO:0008285 negative regulation of cell proliferation GO:0009408 response to heat GO:0018119 peptidyl-cysteine S-nitrosylation GO:0031284 positive regulation of guanylate cyclase activity GO:0033137 negative regulation of peptidyl-serine phosphorylation GO:0033555 multicellular organismal response to stress GO:0035066 positive regulation of histone acetylation GO:0042311 vasodilation GO:0042738 exogenous drug catabolic process GO:0043066 negative regulation of apoptotic process GO:0043267 negative regulation of potassium ion transport GO:0043434 response to peptide hormone GO:0043627 response to estrogen GO:0045184 establishment of protein localization GO:0045776 negative regulation of blood pressure GO:0045822 negative regulation of heart contraction GO:0045893 positive regulation of transcription, DNA-templated GO:0045906 negative regulation of vasoconstriction GO:0045944 positive regulation of transcription from RNA polymerase II promoter GO:0046676 negative regulation of insulin secretion GO:0048148 behavioral response to cocaine GO:0050767 regulation of neurogenesis GO:0051346 negative regulation of hydrolase activity GO:0051481 negative regulation of cytosolic calcium ion concentration GO:0051612 negative regulation of serotonin uptake GO:0051926 negative regulation of calcium ion transport GO:0051930 regulation of sensory perception of pain GO:0055114 oxidation-reduction process GO:0071260 cellular response to mechanical stimulus GO:0071363 cellular response to growth factor stimulus GO:0071872 cellular response to epinephrine stimulus GO:0098735 positive regulation of the force of heart contraction GO:0098924 retrograde trans-synaptic signaling by nitric oxide GO:1900273 positive regulation of long-term synaptic potentiation GO:1901216 positive regulation of neuron death GO:1902307 positive regulation of sodium ion transmembrane transport
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