S. cerevisiae Gene HIS7 (YBR248C)
  Description: Imidazole glycerol phosphate synthase (glutamine amidotransferase:cyclase), catalyzes the fifth and sixth steps of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
Transcript (Including UTRs)
   Position: sacCer3 chrII:714,807-716,465 Size: 1,659 Total Exon Count: 1 Strand: -
Coding Region
   Position: sacCer3 chrII:714,807-716,465 Size: 1,659 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersProtein StructureOther Species
GO AnnotationsOther NamesMethods
Data last updated at UCSC: 2011-08-29

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chrII:714,807-716,465)mRNAProtein (552 aa)
Gene SorterGenome BrowserOther Species FASTAAlphaFoldSGDUniProtKB

-  Comments and Description Text from UniProtKB
  ID: HIS5_YEAST
DESCRIPTION: RecName: Full=Imidazole glycerol phosphate synthase hisHF; Short=IGP synthase; Short=IGPS; Short=ImGP synthase; Includes: RecName: Full=Glutamine amidotransferase; EC=2.4.2.-; Includes: RecName: Full=Cyclase; EC=4.1.3.-;
FUNCTION: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The glutamine amidotransferase domain provides the ammonia necessary to the cyclase domain to produce IGP and AICAR from PRFAR.
CATALYTIC ACTIVITY: 5-[(5-phospho-1-deoxyribulos-1- ylamino)methylideneamino]-1-(5-phosphoribosyl)imidazole-4- carboxamide + L-glutamine = imidazole-glycerol phosphate + 5- aminoimidazol-4-carboxamide ribonucleotide + L-glutamate + H(2)O.
PATHWAY: Amino-acid biosynthesis; L-histidine biosynthesis; L- histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9.
SUBUNIT: Monomer.
INDUCTION: By amino acid starvation. It has a GCN4-dependent and a GCN4-independent (basal) expression.
MISCELLANEOUS: Present with 11800 molecules/cell in log phase SD medium.
SIMILARITY: In the C-terminal section; belongs to the HisA/HisF family.
SIMILARITY: Contains 1 glutamine amidotransferase type-1 domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR013785 - Aldolase_TIM
IPR017926 - GATASE_1
IPR006062 - His_biosynth
IPR004651 - HisF
IPR014640 - IGPS_HisHF
IPR010139 - Imidazole-glycPsynth_HisH
IPR011060 - RibuloseP-bd_barrel

Pfam Domains:
PF00117 - Glutamine amidotransferase class-I
PF00977 - Histidine biosynthesis protein

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1JVN - X-ray 1OX4 - X-ray 1OX5 - X-ray 1OX6 - X-ray


ModBase Predicted Comparative 3D Structure on P33734
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
HumanMouseRatZebrafishD. melanogasterC. elegans
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000107 imidazoleglycerol-phosphate synthase activity
GO:0003824 catalytic activity
GO:0004359 glutaminase activity
GO:0016740 transferase activity
GO:0016763 transferase activity, transferring pentosyl groups
GO:0016829 lyase activity
GO:0016833 oxo-acid-lyase activity

Biological Process:
GO:0000105 histidine biosynthetic process
GO:0006541 glutamine metabolic process
GO:0008152 metabolic process
GO:0008652 cellular amino acid biosynthetic process

Cellular Component:
GO:0005622 intracellular
GO:0005737 cytoplasm


-  Other Names for This Gene
  UCSC ID: YBR248C
Protein: P33734 (aka HIS5_YEAST)

-  SGD Genes Methods, Credits, and Data Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.