Human Gene WNT8B (uc001krb.3)
  Description: Homo sapiens wingless-type MMTV integration site family, member 8B (WNT8B), mRNA.
RefSeq Summary (NM_003393): The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq, Jul 2008].
Transcript (Including UTRs)
   Position: hg19 chr10:102,222,812-102,243,399 Size: 20,588 Total Exon Count: 6 Strand: +
Coding Region
   Position: hg19 chr10:102,222,926-102,242,573 Size: 19,648 Coding Exon Count: 6 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:102,222,812-102,243,399)mRNA (may differ from genome)Protein (351 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCHPRDLynxMGIneXtProt
OMIMPubMedReactomeTreefamUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WNT8B_HUMAN
DESCRIPTION: RecName: Full=Protein Wnt-8b; Flags: Precursor;
FUNCTION: Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.
SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
TISSUE SPECIFICITY: Expression is restricted to the brain, and more specifically to the forebrain.
SIMILARITY: Belongs to the Wnt family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): WNT8B
CDC HuGE Published Literature: WNT8B

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 1.05 RPKM in Testis
Total median expression: 3.99 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -33.20114-0.291 Picture PostScript Text
3' UTR -269.74826-0.327 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR005817 - Wnt
IPR013301 - Wnt8
IPR018161 - Wnt_CS

Pfam Domains:
PF00110 - wnt family

ModBase Predicted Comparative 3D Structure on Q93098
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologGenome BrowserNo orthologNo orthologNo ortholog
Gene Details     
Gene Sorter     
  Ensembl   
  Protein Sequence   
  Alignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005102 receptor binding
GO:0005109 frizzled binding
GO:0048018 receptor agonist activity

Biological Process:
GO:0007165 signal transduction
GO:0007275 multicellular organism development
GO:0007369 gastrulation
GO:0007399 nervous system development
GO:0010469 regulation of receptor activity
GO:0016055 Wnt signaling pathway
GO:0030182 neuron differentiation
GO:0032355 response to estradiol
GO:0032526 response to retinoic acid
GO:0045165 cell fate commitment
GO:0048263 determination of dorsal identity
GO:0060070 canonical Wnt signaling pathway
GO:0071300 cellular response to retinoic acid
GO:1904886 beta-catenin destruction complex disassembly

Cellular Component:
GO:0005576 extracellular region
GO:0005615 extracellular space


-  Descriptions from all associated GenBank mRNAs
  Y11094 - H.sapiens mRNA for WNT-8B protein.
AB073637 - Homo sapiens mRNA for WNT8B, complete cds.
GQ891471 - Homo sapiens clone HEL-S-192 epididymis secretory sperm binding protein mRNA, complete cds.
BC156632 - Synthetic construct Homo sapiens clone IMAGE:100062136, MGC:190176 wingless-type MMTV integration site family, member 8B (WNT8B) mRNA, encodes complete protein.
X91940 - H.sapiens mRNA for WNT-8B protein.
JD204331 - Sequence 185355 from Patent EP1572962.
JD235608 - Sequence 216632 from Patent EP1572962.
JD148671 - Sequence 129695 from Patent EP1572962.
JD311055 - Sequence 292079 from Patent EP1572962.
JD310778 - Sequence 291802 from Patent EP1572962.
JD154611 - Sequence 135635 from Patent EP1572962.
JD357009 - Sequence 338033 from Patent EP1572962.
JD549831 - Sequence 530855 from Patent EP1572962.
JD500401 - Sequence 481425 from Patent EP1572962.
JD172815 - Sequence 153839 from Patent EP1572962.
JD433344 - Sequence 414368 from Patent EP1572962.
JD298424 - Sequence 279448 from Patent EP1572962.
JD262977 - Sequence 244001 from Patent EP1572962.
JD279594 - Sequence 260618 from Patent EP1572962.
JD489545 - Sequence 470569 from Patent EP1572962.
JD555388 - Sequence 536412 from Patent EP1572962.
JD521036 - Sequence 502060 from Patent EP1572962.
JD332959 - Sequence 313983 from Patent EP1572962.
JD486166 - Sequence 467190 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04310 - Wnt signaling pathway
hsa04340 - Hedgehog signaling pathway
hsa04916 - Melanogenesis
hsa05200 - Pathways in cancer
hsa05217 - Basal cell carcinoma

BioCarta from NCI Cancer Genome Anatomy Project
h_wnt-lrp6Pathway - Wnt/LRP6 Signalling

Reactome (by CSHL, EBI, and GO)

Protein Q93098 (Reactome details) participates in the following event(s):

R-HSA-201708 Frizzled receptors bind Wnts
R-HSA-3238694 PORCN palmitoleoylates N-glycosyl WNTs
R-HSA-1458875 WNT binds to FZD and LRP5/6
R-HSA-3247843 secretion of WNT ligands
R-HSA-3247840 WLS binds WNT ligands in the Golgi
R-HSA-1504188 FZD recruits DVL to the receptor complex
R-HSA-201691 Phosphorylation of LRP5/6 cytoplasmic domain by CSNKI
R-NUL-1458902 frog CK1gamma phosphorylates LRP5/6
R-HSA-201677 Phosphorylation of LRP5/6 cytoplasmic domain by membrane-associated GSK3beta
R-HSA-1504186 DVL recruits GSK3beta:AXIN1 to the receptor complex
R-HSA-373080 Class B/2 (Secretin family receptors)
R-HSA-3238698 WNT ligand biogenesis and trafficking
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-500792 GPCR ligand binding
R-HSA-195721 Signaling by WNT
R-HSA-4641262 Disassembly of the destruction complex and recruitment of AXIN to the membrane
R-HSA-372790 Signaling by GPCR
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: NM_003393, NP_003384, O00771, Q5VX55, Q8WYK9, Q93098, WNT8B_HUMAN
UCSC ID: uc001krb.3
RefSeq Accession: NM_003393
Protein: Q93098 (aka WNT8B_HUMAN or WN8B_HUMAN)
CCDS: CCDS7494.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_003393.3
exon count: 6CDS single in 3' UTR: no RNA size: 1996
ORF size: 1056CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 2312.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.