Human Gene HSPA4 (uc003kyj.3)
  Description: Homo sapiens heat shock 70kDa protein 4 (HSPA4), mRNA.
Transcript (Including UTRs)
   Position: hg19 chr5:132,387,662-132,440,709 Size: 53,048 Total Exon Count: 19 Strand: +
Coding Region
   Position: hg19 chr5:132,387,943-132,440,128 Size: 52,186 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsMalaCards
CTDGene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein Structure
Other SpeciesGO AnnotationsmRNA DescriptionsPathwaysOther NamesModel Information
Methods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr5:132,387,662-132,440,709)mRNA (may differ from genome)Protein (840 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
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MGIneXtProtOMIMPubMedReactomeTreefam
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: HSP74_HUMAN
DESCRIPTION: RecName: Full=Heat shock 70 kDa protein 4; AltName: Full=HSP70RY; AltName: Full=Heat shock 70-related protein APG-2;
SUBUNIT: Interacts with TJP1/ZO-1 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (Probable).
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the heat shock protein 70 family.
SEQUENCE CAUTION: Sequence=AAA02807.1; Type=Frameshift; Positions=567, 586, 697, 715, 722;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): HSPA4
CDC HuGE Published Literature: HSPA4
Positive Disease Associations: preterm delivery
Related Studies:
  1. preterm delivery
    Kalish, R. B. et al. 2004, Polymorphisms in the tumor necrosis factor-alpha gene at position -308 and the inducible 70 kd heat shock protein gene at position +1267 in multifetal pregnancies and preterm premature rupture of fetal membranes., American journal of obstetrics and gynecology. 2004 Oct;191(4):1368-74. [PubMed 15507967]
    Tumor necrosis factor-alpha allele 2 and/or heat shock protein-70 allele 2 carriage by the first-born fetus is associated with preterm premature rupture of membranes in multifetal pregnancies.

-  MalaCards Disease Associations
  MalaCards Gene Search: HSPA4
Diseases sorted by gene-association score: ischemia (5), transient cerebral ischemia (4), leishmaniasis (4), salpingitis isthmica nodosa (2), auditory system disease (2), macrocytic anemia, refractory, due to 5q deletion, somatic (2), retinitis pigmentosa 70 (2), retinitis pigmentosa 43 (2), stroke, ischemic (1), retinitis pigmentosa 35 (1), internal hemorrhoid (1), malaria (1), parasitic protozoa infectious disease (1), hypomyelinating leukoencephalopathy (1), retinitis pigmentosa 22 (1), leber congenital amaurosis (1), cogan syndrome (1), photokeratitis (1), acute vascular insufficiency of intestine (1), spinocerebellar ataxia 14 (1), chronic salpingitis (1), amyotrophic lateral sclerosis 1 (0)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 42.26 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 961.29 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -126.70281-0.451 Picture PostScript Text
3' UTR -175.88581-0.303 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR018181 - Heat_shock_70_CS
IPR013126 - Hsp_70_fam

Pfam Domains:
PF00012 - Hsp70 protein
PF06723 - MreB/Mbl protein

SCOP Domains:
100934 - Heat shock protein 70kD (HSP70), C-terminal subdomain
100920 - Heat shock protein 70kD (HSP70), peptide-binding domain
53067 - Actin-like ATPase domain

ModBase Predicted Comparative 3D Structure on P34932
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0005515 protein binding
GO:0005524 ATP binding

Biological Process:
GO:0006986 response to unfolded protein
GO:0045040 protein import into mitochondrial outer membrane
GO:0051131 chaperone-mediated protein complex assembly

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AB023420 - Homo sapiens mRNA for heat shock protein apg-2, complete cds.
FJ224293 - Homo sapiens epididymis secretory sperm binding protein Li 5a (HEL-S-5a) mRNA, complete cds.
BC126122 - Homo sapiens heat shock 70kDa protein 4, mRNA (cDNA clone MGC:161400 IMAGE:8991838), complete cds.
BC126124 - Homo sapiens heat shock 70kDa protein 4, mRNA (cDNA clone MGC:161402 IMAGE:8991840), complete cds.
AB209151 - Homo sapiens mRNA for heat shock 70kDa protein 4 isoform a variant protein.
BC002526 - Homo sapiens heat shock 70kDa protein 4, mRNA (cDNA clone IMAGE:3139645), complete cds.
AK294859 - Homo sapiens cDNA FLJ50510 complete cds, highly similar to Heat shock 70 kDa protein 4.
BC110861 - Homo sapiens heat shock 70kDa protein 4, mRNA (cDNA clone MGC:131852 IMAGE:6169048), complete cds.
L12723 - Homo sapiens heat shock protein 70 (hsp70) mRNA, complete cds.
AK311097 - Homo sapiens cDNA, FLJ18139.
X67643 - H.sapiens HS24/p52 mRNA.
HQ258526 - Synthetic construct Homo sapiens clone IMAGE:100072955 heat shock 70kDa protein 4 (HSPA4) gene, encodes complete protein.
KJ901498 - Synthetic construct Homo sapiens clone ccsbBroadEn_10892 HSPA4 gene, encodes complete protein.
BT007375 - Homo sapiens heat shock protein, 110 kDa mRNA, complete cds.
AK300770 - Homo sapiens cDNA FLJ50691 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK311483 - Homo sapiens cDNA, FLJ18525.
AK295847 - Homo sapiens cDNA FLJ52593 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK311471 - Homo sapiens cDNA, FLJ18513.
AK300050 - Homo sapiens cDNA FLJ54507 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK311224 - Homo sapiens cDNA, FLJ18266.
AK311135 - Homo sapiens cDNA, FLJ18177.
AK304578 - Homo sapiens cDNA FLJ55293 complete cds, highly similar to Heat shock 70 kDa protein 4.
DL492554 - Novel nucleic acids.
AK311299 - Homo sapiens cDNA, FLJ18341.
AK303382 - Homo sapiens cDNA FLJ52584 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK311466 - Homo sapiens cDNA, FLJ18508.
AK311615 - Homo sapiens cDNA, FLJ18657.
AK311632 - Homo sapiens cDNA, FLJ18674.
AB074275 - Homo sapiens primary neuroblastoma cDNA, clone:Nbla10949, full insert sequence.
AK311422 - Homo sapiens cDNA, FLJ18464.
AK311465 - Homo sapiens cDNA, FLJ18507.
AK311125 - Homo sapiens cDNA, FLJ18167.
AK311457 - Homo sapiens cDNA, FLJ18499.
AK299710 - Homo sapiens cDNA FLJ50643 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK315887 - Homo sapiens cDNA, FLJ79536 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK311316 - Homo sapiens cDNA, FLJ18358.
AK315862 - Homo sapiens cDNA, FLJ79511 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK315871 - Homo sapiens cDNA, FLJ79520 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK315875 - Homo sapiens cDNA, FLJ79524 complete cds, highly similar to Heat shock 70 kDa protein 4.
AK297762 - Homo sapiens cDNA FLJ50587 complete cds, highly similar to Heat shock 70 kDa protein 4.
JD438719 - Sequence 419743 from Patent EP1572962.
JD349862 - Sequence 330886 from Patent EP1572962.
JD020093 - Sequence 1117 from Patent EP1572962.
JD032753 - Sequence 13777 from Patent EP1572962.
JD532581 - Sequence 513605 from Patent EP1572962.
JD057655 - Sequence 38679 from Patent EP1572962.
JD271933 - Sequence 252957 from Patent EP1572962.
JD365901 - Sequence 346925 from Patent EP1572962.
JD131518 - Sequence 112542 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04612 - Antigen processing and presentation

Reactome (by CSHL, EBI, and GO)

Protein P34932 (Reactome details) participates in the following event(s):

R-HSA-5251942 Hikeshi binds HSP70s:ATP
R-HSA-5251959 HSP40s activate intrinsic ATPase activity of HSP70s in the cytosol
R-HSA-5252079 HSP110s exchange ATP for ADP on HSP70s:ADP
R-HSA-5251955 HSP40s activate intrinsic ATPase activity of HSP70s in the nucleoplasm
R-HSA-5252041 NPC transports Hikeshi:HSP70s:ATP from cytosol to nucleoplasm
R-HSA-3371453 Regulation of HSF1-mediated heat shock response
R-HSA-3371556 Cellular response to heat stress
R-HSA-2262752 Cellular responses to stress
R-HSA-8953897 Cellular responses to external stimuli

-  Other Names for This Gene
  Alternate Gene Symbols: APG2, HSP74_HUMAN, NM_002154, NP_002145, O95756, P34932, Q2TAL4
UCSC ID: uc003kyj.3
RefSeq Accession: NM_002154
Protein: P34932 (aka HSP74_HUMAN)
CCDS: CCDS4166.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_002154.3
exon count: 19CDS single in 3' UTR: no RNA size: 3385
ORF size: 2523CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 4778.00frame shift in genome: no % Coverage: 100.00
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.