Human Gene CLASP1 (uc002tnc.3)
  Description: Homo sapiens cytoplasmic linker associated protein 1 (CLASP1), transcript variant 1, mRNA.
RefSeq Summary (NM_015282): CLASPs, such as CLASP1, are nonmotor microtubule-associated proteins that interact with CLIPs (e.g., CLIP170; MIM 179838). CLASP1 is involved in the regulation of microtubule dynamics at the kinetochore and throughout the spindle (Maiato et al., 2003 [PubMed 12837247]).[supplied by OMIM, Mar 2008].
Transcript (Including UTRs)
   Position: hg19 chr2:122,095,352-122,407,052 Size: 311,701 Total Exon Count: 39 Strand: -
Coding Region
   Position: hg19 chr2:122,098,437-122,363,471 Size: 265,035 Coding Exon Count: 38 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:122,095,352-122,407,052)mRNA (may differ from genome)Protein (1538 aa)
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-  Comments and Description Text from UniProtKB
  ID: CLAP1_HUMAN
DESCRIPTION: RecName: Full=CLIP-associating protein 1; AltName: Full=Cytoplasmic linker-associated protein 1; AltName: Full=Multiple asters homolog 1; AltName: Full=Protein Orbit homolog 1; Short=hOrbit1;
FUNCTION: Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle.
SUBUNIT: Interacts with CLIP2, ERC1, MAPRE1, MAPRE3, microtubules, PHLDB2 and RSN. The interaction with ERC1 may be mediated by PHLDB2. Interacts with GCC2; recruits CLASP1 to Golgi membranes. Interacts with MACF1 (By similarity).
INTERACTION: Q9JK25:Clip1 (xeno); NbExp=2; IntAct=EBI-913476, EBI-908338; O55156:Clip2 (xeno); NbExp=3; IntAct=EBI-913476, EBI-349416;
SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, centrosome. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Golgi apparatus, trans-Golgi network (Probable). Note=Localizes to microtubule plus ends. Localizes to centrosomes, kinetochores and the mitotic spindle from prometaphase. Subsequently localizes to the spindle midzone from anaphase and to the midbody from telophase. In migrating cells localizes to the plus ends of microtubules within the cell body and to the entire microtubule lattice within the lamella. Localizes to the cell cortex and this requires ERC1 and PHLDB2.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the CLASP family.
SIMILARITY: Contains 7 HEAT repeats.
SEQUENCE CAUTION: Sequence=AAH32563.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=AAX88872.1; Type=Erroneous gene model prediction;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): CLASP1
CDC HuGE Published Literature: CLASP1
Positive Disease Associations: Coronary Disease
Related Studies:
  1. Coronary Disease
    , , . [PubMed 0]

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 23.32 RPKM in Brain - Cerebellar Hemisphere
Total median expression: 501.10 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -105.41390-0.270 Picture PostScript Text
3' UTR -947.113085-0.307 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011989 - ARM-like
IPR016024 - ARM-type_fold
IPR024395 - CLASP_N_dom
IPR000357 - HEAT
IPR021133 - HEAT_type_2

Pfam Domains:
PF02985 - HEAT repeat
PF12348 - CLASP N terminal

SCOP Domains:
48371 - ARM repeat
48431 - Lipovitellin-phosvitin complex, superhelical domain

ModBase Predicted Comparative 3D Structure on Q7Z460
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-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologGenome BrowserGenome BrowserNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGDEnsembl   
 Protein SequenceProtein Sequence   
 AlignmentAlignment   

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0002162 dystroglycan binding
GO:0005515 protein binding
GO:0008017 microtubule binding
GO:0043515 kinetochore binding
GO:0051010 microtubule plus-end binding

Biological Process:
GO:0000086 G2/M transition of mitotic cell cycle
GO:0000226 microtubule cytoskeleton organization
GO:0001578 microtubule bundle formation
GO:0006903 vesicle targeting
GO:0007020 microtubule nucleation
GO:0007026 negative regulation of microtubule depolymerization
GO:0007030 Golgi organization
GO:0007049 cell cycle
GO:0007052 mitotic spindle organization
GO:0007163 establishment or maintenance of cell polarity
GO:0010389 regulation of G2/M transition of mitotic cell cycle
GO:0010458 exit from mitosis
GO:0010470 regulation of gastrulation
GO:0010634 positive regulation of epithelial cell migration
GO:0010717 regulation of epithelial to mesenchymal transition
GO:0030953 astral microtubule organization
GO:0031023 microtubule organizing center organization
GO:0031111 negative regulation of microtubule polymerization or depolymerization
GO:0031116 positive regulation of microtubule polymerization
GO:0034453 microtubule anchoring
GO:0040001 establishment of mitotic spindle localization
GO:0045921 positive regulation of exocytosis
GO:0051294 establishment of spindle orientation
GO:0051301 cell division
GO:0051497 negative regulation of stress fiber assembly
GO:0051893 regulation of focal adhesion assembly
GO:0070507 regulation of microtubule cytoskeleton organization
GO:0090091 positive regulation of extracellular matrix disassembly
GO:0090162 establishment of epithelial cell polarity
GO:0090307 mitotic spindle assembly
GO:0097711 ciliary basal body docking
GO:1903690 negative regulation of wound healing, spreading of epidermal cells
GO:1904261 positive regulation of basement membrane assembly involved in embryonic body morphogenesis

Cellular Component:
GO:0000775 chromosome, centromeric region
GO:0000776 kinetochore
GO:0000777 condensed chromosome kinetochore
GO:0005694 chromosome
GO:0005737 cytoplasm
GO:0005794 Golgi apparatus
GO:0005813 centrosome
GO:0005815 microtubule organizing center
GO:0005819 spindle
GO:0005828 kinetochore microtubule
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005874 microtubule
GO:0005876 spindle microtubule
GO:0005881 cytoplasmic microtubule
GO:0005938 cell cortex
GO:0016020 membrane
GO:0030981 cortical microtubule cytoskeleton
GO:0031592 centrosomal corona
GO:0035371 microtubule plus-end
GO:0045180 basal cortex
GO:0005925 focal adhesion


-  Descriptions from all associated GenBank mRNAs
  CR749627 - Homo sapiens mRNA; cDNA DKFZp686D1968 (from clone DKFZp686D1968).
BX648933 - Homo sapiens mRNA; cDNA DKFZp686A1844 (from clone DKFZp686A1844).
LF383633 - JP 2014500723-A/191136: Polycomb-Associated Non-Coding RNAs.
AB014522 - Homo sapiens mRNA for KIAA0622 protein, partial cds.
AF347693 - Homo sapiens multiple asters 1 (MAST1) mRNA, complete cds.
AK074338 - Homo sapiens cDNA FLJ23758 fis, clone HEP18066.
AK091140 - Homo sapiens cDNA FLJ33821 fis, clone CTONG2003293, weakly similar to Microtubule associated-protein orbit.
AX746802 - Sequence 327 from Patent EP1308459.
BC132723 - Homo sapiens cytoplasmic linker associated protein 1, mRNA (cDNA clone MGC:164354 IMAGE:40146745), complete cds.
BC144107 - Homo sapiens cytoplasmic linker associated protein 1, mRNA (cDNA clone MGC:177644 IMAGE:9052627), complete cds.
AK294922 - Homo sapiens cDNA FLJ55778 complete cds, highly similar to CLIP-associating protein 1.
BC112940 - Homo sapiens cytoplasmic linker associated protein 1, mRNA (cDNA clone MGC:131895 IMAGE:5501463), complete cds.
AK299331 - Homo sapiens cDNA FLJ61355 complete cds, highly similar to CLIP-associating protein 1.
AK297259 - Homo sapiens cDNA FLJ55338 complete cds, highly similar to CLIP-associating protein 1.
CR933722 - Homo sapiens mRNA; cDNA DKFZp686H2039 (from clone DKFZp686H2039).
AK302393 - Homo sapiens cDNA FLJ53242 complete cds, highly similar to CLIP-associating protein 1.
AB384508 - Synthetic construct DNA, clone: pF1KA0622, Homo sapiens CLASP1 gene for CLIP-associating protein 1, complete cds, without stop codon, in Flexi system.
AJ288057 - Homo sapiens partial mRNA for CLIP-associating protein CLASP1, alternatively spliced.
DQ200867 - Homo sapiens CLIP-associated protein 1 beta mRNA, partial cds.
MA619210 - JP 2018138019-A/191136: Polycomb-Associated Non-Coding RNAs.
BC032563 - Homo sapiens cytoplasmic linker associated protein 1, mRNA (cDNA clone IMAGE:5579440), complete cds.
JD239180 - Sequence 220204 from Patent EP1572962.
JD545859 - Sequence 526883 from Patent EP1572962.
JD090945 - Sequence 71969 from Patent EP1572962.
JD280525 - Sequence 261549 from Patent EP1572962.
JD141437 - Sequence 122461 from Patent EP1572962.
JD422614 - Sequence 403638 from Patent EP1572962.
JD134711 - Sequence 115735 from Patent EP1572962.
JD423997 - Sequence 405021 from Patent EP1572962.
JD209552 - Sequence 190576 from Patent EP1572962.
JD195025 - Sequence 176049 from Patent EP1572962.
JD452004 - Sequence 433028 from Patent EP1572962.
JD186282 - Sequence 167306 from Patent EP1572962.
JD039846 - Sequence 20870 from Patent EP1572962.
JD433625 - Sequence 414649 from Patent EP1572962.
JD458626 - Sequence 439650 from Patent EP1572962.
JD263891 - Sequence 244915 from Patent EP1572962.
JD329436 - Sequence 310460 from Patent EP1572962.
JD329435 - Sequence 310459 from Patent EP1572962.
JD405992 - Sequence 387016 from Patent EP1572962.
JD061535 - Sequence 42559 from Patent EP1572962.
JD457724 - Sequence 438748 from Patent EP1572962.
JD314253 - Sequence 295277 from Patent EP1572962.
JD431156 - Sequence 412180 from Patent EP1572962.
JD290203 - Sequence 271227 from Patent EP1572962.
JD328388 - Sequence 309412 from Patent EP1572962.
JD127364 - Sequence 108388 from Patent EP1572962.
JD122466 - Sequence 103490 from Patent EP1572962.
JD468924 - Sequence 449948 from Patent EP1572962.
JD274140 - Sequence 255164 from Patent EP1572962.
JD392205 - Sequence 373229 from Patent EP1572962.
JD316766 - Sequence 297790 from Patent EP1572962.
JD284372 - Sequence 265396 from Patent EP1572962.
JD375715 - Sequence 356739 from Patent EP1572962.
JD180740 - Sequence 161764 from Patent EP1572962.
JD507601 - Sequence 488625 from Patent EP1572962.
JD172073 - Sequence 153097 from Patent EP1572962.
JD528486 - Sequence 509510 from Patent EP1572962.
CQ873825 - Sequence 244 from Patent WO2004076622.
DD413662 - Regulation of Mammalian Cells.
JD095831 - Sequence 76855 from Patent EP1572962.
JD380429 - Sequence 361453 from Patent EP1572962.
JD439789 - Sequence 420813 from Patent EP1572962.
JD111967 - Sequence 92991 from Patent EP1572962.
JD387046 - Sequence 368070 from Patent EP1572962.
JD165918 - Sequence 146942 from Patent EP1572962.
JD441592 - Sequence 422616 from Patent EP1572962.
JD538402 - Sequence 519426 from Patent EP1572962.
AK301814 - Homo sapiens cDNA FLJ56898 complete cds, highly similar to CLIP-associating protein 1.
JD362004 - Sequence 343028 from Patent EP1572962.
AK123216 - Homo sapiens cDNA FLJ41222 fis, clone BRAMY2029204.
AK297420 - Homo sapiens cDNA FLJ55790 complete cds, highly similar to CLIP-associating protein 1.
JD115872 - Sequence 96896 from Patent EP1572962.
JD090196 - Sequence 71220 from Patent EP1572962.
JD183035 - Sequence 164059 from Patent EP1572962.
KJ902326 - Synthetic construct Homo sapiens clone ccsbBroadEn_11720 CLASP1 gene, encodes complete protein.
JD321509 - Sequence 302533 from Patent EP1572962.
JD141506 - Sequence 122530 from Patent EP1572962.
JD250537 - Sequence 231561 from Patent EP1572962.
JD058870 - Sequence 39894 from Patent EP1572962.
BX648523 - Homo sapiens mRNA; cDNA DKFZp686G1385 (from clone DKFZp686G1385).
JD277234 - Sequence 258258 from Patent EP1572962.
JD400183 - Sequence 381207 from Patent EP1572962.
JD444989 - Sequence 426013 from Patent EP1572962.
JD048660 - Sequence 29684 from Patent EP1572962.
JD500565 - Sequence 481589 from Patent EP1572962.
JD058346 - Sequence 39370 from Patent EP1572962.
JD309509 - Sequence 290533 from Patent EP1572962.
JD551926 - Sequence 532950 from Patent EP1572962.
JD131460 - Sequence 112484 from Patent EP1572962.
JD115353 - Sequence 96377 from Patent EP1572962.
JD091729 - Sequence 72753 from Patent EP1572962.
JD037372 - Sequence 18396 from Patent EP1572962.
JD567110 - Sequence 548134 from Patent EP1572962.
JD043388 - Sequence 24412 from Patent EP1572962.
JD241685 - Sequence 222709 from Patent EP1572962.
AY425956 - Homo sapiens MAST1 mRNA, partial cds, alternatively spliced.
JD150167 - Sequence 131191 from Patent EP1572962.
JD433487 - Sequence 414511 from Patent EP1572962.
JD146021 - Sequence 127045 from Patent EP1572962.
JD349948 - Sequence 330972 from Patent EP1572962.
JD299326 - Sequence 280350 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q7Z460 (Reactome details) participates in the following event(s):

R-HSA-141409 Mad1 binds kinetochore
R-HSA-375302 Kinetochore capture of astral microtubules
R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores
R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2
R-HSA-380272 Plk1-mediated phosphorylation of Nlp
R-HSA-380283 Recruitment of additional gamma tubulin/ gamma TuRC to the centrosome
R-HSA-380294 Loss of C-Nap-1 from centrosomes
R-HSA-380311 Recruitment of Plk1 to centrosomes
R-HSA-380455 Recruitment of CDK11p58 to the centrosomes
R-HSA-380303 Dissociation of Phospho-Nlp from the centrosome
R-HSA-5626220 C2CD3 binds the mother centriole
R-NUL-428884 Activation of Clasp
R-HSA-428885 Activation of CLASP
R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex
R-HSA-141439 Release of activated MAD2 from kinetochores
R-HSA-2467811 Separation of sister chromatids
R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin
R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores
R-HSA-380508 Translocation of NuMA to the centrosomes
R-HSA-2574845 AJUBA binds centrosome-associated AURKA
R-HSA-8853405 TPX2 binds AURKA at centrosomes
R-HSA-3000319 BORA binds PLK1 and AURKA
R-HSA-2574840 AJUBA facilitates AURKA autophosphorylation
R-HSA-3000310 AURKA phosphorylates PLK1
R-HSA-5626223 C2CD3 and OFD1 recruit 5 distal appendage proteins to the centriole
R-HSA-5626681 Recruitment of transition zone proteins
R-HSA-5626227 CP110 and CEP97 dissociate from the centriole
R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1
R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin
R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres
R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres
R-HSA-380316 Association of NuMA with microtubules
R-HSA-8853419 TPX2 promotes AURKA autophosphorylation
R-HSA-5626228 The distal appendage proteins recruit TTBK2
R-HSA-5638009 CEP164 recruits RAB3IP-carrying Golgi-derived vesicles to the basal body
R-HSA-5626699 MARK4 binds ODF2 in the centriole
R-HSA-5617816 RAB3IP stimulates nucleotide exchange on RAB8A
R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-HSA-68877 Mitotic Prometaphase
R-HSA-5663220 RHO GTPases Activate Formins
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-428890 Role of ABL in ROBO-SLIT signaling
R-HSA-2500257 Resolution of Sister Chromatid Cohesion
R-HSA-2467813 Separation of Sister Chromatids
R-HSA-141424 Amplification of signal from the kinetochores
R-HSA-68886 M Phase
R-HSA-195258 RHO GTPase Effectors
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-8854518 AURKA Activation by TPX2
R-HSA-380287 Centrosome maturation
R-HSA-5617833 Cilium Assembly
R-HSA-376176 Signaling by ROBO receptors
R-HSA-68882 Mitotic Anaphase
R-HSA-69618 Mitotic Spindle Checkpoint
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-194315 Signaling by Rho GTPases
R-HSA-69275 G2/M Transition
R-HSA-1852241 Organelle biogenesis and maintenance
R-HSA-422475 Axon guidance
R-HSA-2555396 Mitotic Metaphase and Anaphase
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-1640170 Cell Cycle
R-HSA-162582 Signal Transduction
R-HSA-453274 Mitotic G2-G2/M phases
R-HSA-1266738 Developmental Biology

-  Other Names for This Gene
  Alternate Gene Symbols: CLAP1_HUMAN, KIAA0622, MAST1, NM_015282, NP_056097, O75118, Q2KHQ9, Q5H9P0, Q7Z460, Q8N5B8, Q9BQT5
UCSC ID: uc002tnc.3
RefSeq Accession: NM_015282
Protein: Q7Z460 (aka CLAP1_HUMAN)

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_015282.2
exon count: 39CDS single in 3' UTR: no RNA size: 8118
ORF size: 4618CDS single in intron: no Alignment % ID: 99.98
txCdsPredict score: 5164.00frame shift in genome: no % Coverage: 99.64
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.