Human Gene CDC7 (uc001dof.3)
  Description: Homo sapiens cell division cycle 7 (CDC7), transcript variant 2, mRNA.
RefSeq Summary (NM_001134419): This gene encodes a cell division cycle protein with kinase activity that is critical for the G1/S transition. The yeast homolog is also essential for initiation of DNA replication as cell division occurs. Overexpression of this gene product may be associated with neoplastic transformation for some tumors. Multiple alternatively spliced transcript variants that encode the same protein have been detected. [provided by RefSeq, Aug 2008].
Transcript (Including UTRs)
   Position: hg19 chr1:91,966,404-91,991,321 Size: 24,918 Total Exon Count: 12 Strand: +
Coding Region
   Position: hg19 chr1:91,967,274-91,989,992 Size: 22,719 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersGenetic AssociationsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2013-06-14

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:91,966,404-91,991,321)mRNA (may differ from genome)Protein (574 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
GeneNetworkHGNCLynxMGIneXtProtOMIM
PubMedReactomeTreefamUniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: CDC7_HUMAN
DESCRIPTION: RecName: Full=Cell division cycle 7-related protein kinase; Short=CDC7-related kinase; Short=HsCdc7; Short=huCdc7; EC=2.7.11.1;
FUNCTION: Seems to phosphorylate critical substrates that regulate the G1/S phase transition and/or DNA replication. Can phosphorylates MCM2 and MCM3.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
SUBUNIT: Forms a complex with either DBF4/DBF4A or DBF4B, leading to the activation of the kinase activity.
SUBCELLULAR LOCATION: Nucleus.
SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. CDC7 subfamily.
SIMILARITY: Contains 1 protein kinase domain.
WEB RESOURCE: Name=NIEHS-SNPs; URL="http://egp.gs.washington.edu/data/cdc7/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Genetic Association Studies of Complex Diseases and Disorders
  Genetic Association Database (archive): CDC7
CDC HuGE Published Literature: CDC7
Positive Disease Associations: Blood Pressure , Body Mass Index , Body Weight
Related Studies:
  1. Blood Pressure
    Daniel Levy et al. BMC medical genetics 2007, Framingham Heart Study 100K Project: genome-wide associations for blood pressure and arterial stiffness., BMC medical genetics. [PubMed 17903302]
    These results of genome-wide association testing for blood pressure and arterial stiffness phenotypes in an unselected community-based sample of adults may aid in the identification of the genetic basis of hypertension and arterial disease, help identify high risk individuals, and guide novel therapies for hypertension. Additional studies are needed to replicate any associations identified in these analyses.
  2. Body Mass Index
    , , . [PubMed 0]
  3. Body Weight
    , , . [PubMed 0]
           more ... click here to view the complete list

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene
  • D001564 Benzo(a)pyrene
  • D013749 Tetrachlorodibenzodioxin
  • C006780 bisphenol A
  • C029497 2,3-bis(3'-hydroxybenzyl)butyrolactone
  • C532162 2-(1H-indazol-4-yl)-6-(4-methanesulfonylpiperazin-1-ylmethyl)-4-morpholin-4-ylthieno(3,2-d)pyrimidine
  • C009505 4,4'-diaminodiphenylmethane
  • D015112 4-Nitroquinoline-1-oxide
  • D015123 7,8-Dihydro-7,8-dihydroxybenzo(a)pyrene 9,10-oxide
  • C553817 7-(benzylamino)-1,3,4,8-tetrahydropyrrolo(4,3,2-de)quinolin-8(1H)-one
  • C547126 AZM551248
          more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 11.65 RPKM in Testis
Total median expression: 117.09 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -41.02131-0.313 Picture PostScript Text
3' UTR -327.221329-0.246 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR011009 - Kinase-like_dom
IPR000719 - Prot_kinase_cat_dom
IPR017441 - Protein_kinase_ATP_BS
IPR008271 - Ser/Thr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF07714 - Protein tyrosine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on O00311
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0046872 metal ion binding

Biological Process:
GO:0000082 G1/S transition of mitotic cell cycle
GO:0000727 double-strand break repair via break-induced replication
GO:0006260 DNA replication
GO:0006270 DNA replication initiation
GO:0006468 protein phosphorylation
GO:0006909 phagocytosis
GO:0007049 cell cycle
GO:0008284 positive regulation of cell proliferation
GO:0008360 regulation of cell shape
GO:0010571 positive regulation of nuclear cell cycle DNA replication
GO:0010971 positive regulation of G2/M transition of mitotic cell cycle
GO:0016310 phosphorylation
GO:0018105 peptidyl-serine phosphorylation
GO:0044770 cell cycle phase transition
GO:0051301 cell division
GO:0070317 negative regulation of G0 to G1 transition

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0045171 intercellular bridge
GO:0072686 mitotic spindle


-  Descriptions from all associated GenBank mRNAs
  AK298963 - Homo sapiens cDNA FLJ51946 complete cds, highly similar to Cell division cycle 7-related protein kinase (EC 2.7.11.1).
BC111044 - Homo sapiens cell division cycle 7 homolog (S. cerevisiae), mRNA (cDNA clone MGC:117361 IMAGE:5518616), complete cds.
GQ900903 - Homo sapiens clone HEL-T-15 epididymis secretory sperm binding protein mRNA, complete cds.
AB209337 - Homo sapiens mRNA for CDC7 cell division cycle 7 variant protein.
AF015592 - Homo sapiens Cdc7 (CDC7) mRNA, complete cds.
BC110526 - Homo sapiens cell division cycle 7 homolog (S. cerevisiae), mRNA (cDNA clone MGC:126237 IMAGE:40034178), complete cds.
BC110527 - Homo sapiens cell division cycle 7 homolog (S. cerevisiae), mRNA (cDNA clone MGC:126238 IMAGE:40034179), complete cds.
AB003698 - Homo sapiens mRNA for Cdc7-related kinase, complete cds.
JD439464 - Sequence 420488 from Patent EP1572962.
AK312726 - Homo sapiens cDNA, FLJ93132, highly similar to Homo sapiens CDC7 cell division cycle 7 (S. cerevisiae) (CDC7), mRNA.
AB590547 - Synthetic construct DNA, clone: pFN21AE0979, Homo sapiens CDC7 gene for cell division cycle 7 homolog, without stop codon, in Flexi system.
KJ897825 - Synthetic construct Homo sapiens clone ccsbBroadEn_07219 CDC7 gene, encodes complete protein.
AF005209 - Homo sapiens HsCdc7 mRNA, complete cds.
AY312583 - Homo sapiens cell division cycle 7-like protein 1 (CDC7) mRNA, partial cds, alternatively spliced.
JD237829 - Sequence 218853 from Patent EP1572962.
JD503323 - Sequence 484347 from Patent EP1572962.
JD291896 - Sequence 272920 from Patent EP1572962.
JD181307 - Sequence 162331 from Patent EP1572962.
JD552012 - Sequence 533036 from Patent EP1572962.
JD294515 - Sequence 275539 from Patent EP1572962.
JD528395 - Sequence 509419 from Patent EP1572962.
JD060602 - Sequence 41626 from Patent EP1572962.
JD276498 - Sequence 257522 from Patent EP1572962.
JD164260 - Sequence 145284 from Patent EP1572962.
JD168449 - Sequence 149473 from Patent EP1572962.
JD314641 - Sequence 295665 from Patent EP1572962.
JD176612 - Sequence 157636 from Patent EP1572962.
JD170816 - Sequence 151840 from Patent EP1572962.
JD208154 - Sequence 189178 from Patent EP1572962.
JD225763 - Sequence 206787 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  KEGG - Kyoto Encyclopedia of Genes and Genomes
hsa04110 - Cell cycle

Reactome (by CSHL, EBI, and GO)

Protein O00311 (Reactome details) participates in the following event(s):

R-HSA-68918 CDK and DDK associate with the Mcm10:pre-replicative complex
R-HSA-68917 Cdc45 associates with the pre-replicative complex at the origin
R-HSA-68954 Mcm2-7 is phosphorylated by DDK
R-HSA-68916 DNA Replication Factor A (RPA) associates with the pre-replicative complex at the origin
R-HSA-176318 Loading of claspin onto DNA during replication origin firing
R-HSA-176298 Activation of claspin
R-HSA-8953750 Transcriptional Regulation by E2F6
R-HSA-68962 Activation of the pre-replicative complex
R-HSA-212436 Generic Transcription Pathway
R-HSA-69002 DNA Replication Pre-Initiation
R-HSA-69206 G1/S Transition
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-176187 Activation of ATR in response to replication stress
R-HSA-68874 M/G1 Transition
R-HSA-453279 Mitotic G1-G1/S phases
R-HSA-74160 Gene expression (Transcription)
R-HSA-69481 G2/M Checkpoints
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-69306 DNA Replication
R-HSA-69620 Cell Cycle Checkpoints
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: CDC7L1, CDC7_HUMAN, D3DT31, NM_001134419, NP_003494, O00311, O00558, Q5T5U5
UCSC ID: uc001dof.3
RefSeq Accession: NM_001134419
Protein: O00311 (aka CDC7_HUMAN)
CCDS: CCDS734.1

-  Gene Model Information
 
category: coding nonsense-mediated-decay: no RNA accession: NM_001134419.1
exon count: 12CDS single in 3' UTR: no RNA size: 3188
ORF size: 1725CDS single in intron: no Alignment % ID: 100.00
txCdsPredict score: 3650.00frame shift in genome: no % Coverage: 99.91
has start codon: yes stop codon in genome: no # of Alignments: 1
has end codon: yes retained intron: no # AT/AC introns 0
selenocysteine: no end bleed into intron: 0# strange splices: 0
Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.