Schema for Euarch Chain/Net - Euarchontoglire Genomes, Chain and Net Alignments
  Database: mm10    Primary Table: chainHg38    Row Count: 3,678,476   Data last updated: 2015-04-10
Format description: Summary info about a chain of alignments
On download server: MariaDB table dump directory
fieldexampleSQL type description
bin 607smallint(5) unsigned Indexing field to speed chromosome range queries.
score 13770double score of chain
tName chr1varchar(255) Target sequence name
tSize 195471971int(10) unsigned Target sequence size
tStart 3007004int(10) unsigned Alignment start position in target
tEnd 3007923int(10) unsigned Alignment end position in target
qName chr8varchar(255) Query sequence name
qSize 145138636int(10) unsigned Query sequence size
qStrand -char(1) Query strand
qStart 119522013int(10) unsigned Alignment start position in query
qEnd 119523064int(10) unsigned Alignment end position in query
id 1254259int(10) unsigned chain id

Connected Tables and Joining Fields
        mm10.chainHg38Link.chainId (via chainHg38.id)
      mm10.netHg38.chainId (via chainHg38.id)

Sample Rows
 
binscoretNametSizetStarttEndqNameqSizeqStrandqStartqEndid
60713770chr119547197130070043007923chr8145138636-1195220131195230641254259
60714535chr119547197130070043007985chr2146709983-21473602214746831135269
6079580chr119547197130071993007781chrX156040895-72123866721243922024146
60718839chr119547197130073383008058chr8145138636-8948854889489248706484
6088462chr119547197130741483074332chr8145138636-89515947895161312225134
916099613chr119547197130945547172812chr8145138636-8952805793321134188
60814825chr119547197131451073145396chr11135086622+1193006361193009701093909
60928052chr119547197131468963147383chr5181538259+6238618062386707337613
60924445chr119547197132160153216979chr2064444167-3244670432447674442100
60927087chr119547197132161143217015chr8145138636+1089804710898978362362

Note: all start coordinates in our database are 0-based, not 1-based. See explanation here.

Euarch Chain/Net (euarchontoglireChainNet) Track Description
 

Description

Chain Track

The chain track shows alignments of mouse (Dec. 2011 (GRCm38/mm10)/mm10) to other genomes using a gap scoring system that allows longer gaps than traditional affine gap scoring systems. It can also tolerate gaps in both mouse and the other genome simultaneously. These "double-sided" gaps can be caused by local inversions and overlapping deletions in both species.

The chain track displays boxes joined together by either single or double lines. The boxes represent aligning regions. Single lines indicate gaps that are largely due to a deletion in the other assembly or an insertion in the mouse assembly. Double lines represent more complex gaps that involve substantial sequence in both species. This may result from inversions, overlapping deletions, an abundance of local mutation, or an unsequenced gap in one species. In cases where multiple chains align over a particular region of the other genome, the chains with single-lined gaps are often due to processed pseudogenes, while chains with double-lined gaps are more often due to paralogs and unprocessed pseudogenes.

In the "pack" and "full" display modes, the individual feature names indicate the chromosome, strand, and location (in thousands) of the match for each matching alignment.

Net Track

The net track shows the best mouse/other chain for every part of the other genome. It is useful for finding orthologous regions and for studying genome rearrangement. The mouse sequence used in this annotation is from the Dec. 2011 (GRCm38/mm10) (mm10) assembly.

Display Conventions and Configuration

Multiple species are grouped together in a composite track. In the display and on the configuration page, an effort was made to group them loosely into "clades." These groupings are based on the taxonomic classification at NCBI, using the CommonTree tool. Some organisms may be pulled from a larger group into a subgroup, to emphasize a relationship. For example, members of an Order may be listed together, while other organisms in the same Superorder may be grouped separately under the Superorder name.

Chain Track

By default, the chains to chromosome-based assemblies are colored based on which chromosome they map to in the aligning organism. To turn off the coloring, check the "off" button next to: Color track based on chromosome.

To display only the chains of one chromosome in the aligning organism, enter the name of that chromosome (e.g. chr4) in box next to: Filter by chromosome.

Net Track

In full display mode, the top-level (level 1) chains are the largest, highest-scoring chains that span this region. In many cases gaps exist in the top-level chain. When possible, these are filled in by other chains that are displayed at level 2. The gaps in level 2 chains may be filled by level 3 chains and so forth.

In the graphical display, the boxes represent ungapped alignments; the lines represent gaps. Click on a box to view detailed information about the chain as a whole; click on a line to display information about the gap. The detailed information is useful in determining the cause of the gap or, for lower level chains, the genomic rearrangement.

Individual items in the display are categorized as one of four types (other than gap):

  • Top - the best, longest match. Displayed on level 1.
  • Syn - line-ups on the same chromosome as the gap in the level above it.
  • Inv - a line-up on the same chromosome as the gap above it, but in the opposite orientation.
  • NonSyn - a match to a chromosome different from the gap in the level above.

Methods

Chain track

Transposons that have been inserted since the mouse/other split were removed from the assemblies. The abbreviated genomes were aligned with lastz, and the transposons were added back in. The resulting alignments were converted into axt format using the lavToAxt program. The axt alignments were fed into axtChain, which organizes all alignments between a single mouse chromosome and a single chromosome from the other genome into a group and creates a kd-tree out of the gapless subsections (blocks) of the alignments. A dynamic program was then run over the kd-trees to find the maximally scoring chains of these blocks.

See also: lastz parameters and other details (e.g., update time) and chain minimum score and gap parameters used in these alignments.

Net track

Chains were derived from lastz alignments, using the methods described on the chain tracks description pages, and sorted with the highest-scoring chains in the genome ranked first. The program chainNet was then used to place the chains one at a time, trimming them as necessary to fit into sections not already covered by a higher-scoring chain. During this process, a natural hierarchy emerged in which a chain that filled a gap in a higher-scoring chain was placed underneath that chain. The program netSyntenic was used to fill in information about the relationship between higher- and lower-level chains, such as whether a lower-level chain was syntenic or inverted relative to the higher-level chain. The program netClass was then used to fill in how much of the gaps and chains contained Ns (sequencing gaps) in one or both species and how much was filled with transposons inserted before and after the two organisms diverged.

Credits

Lastz (previously known as blastz) was developed at Pennsylvania State University by Minmei Hou, Scott Schwartz, Zheng Zhang, and Webb Miller with advice from Ross Hardison.

Lineage-specific repeats were identified by Arian Smit and his RepeatMasker program.

The axtChain program was developed at the University of California at Santa Cruz by Jim Kent with advice from Webb Miller and David Haussler.

The browser display and database storage of the chains and nets were created by Robert Baertsch and Jim Kent.

The chainNet, netSyntenic, and netClass programs were developed at the University of California Santa Cruz by Jim Kent.

References

Chiaromonte F, Yap VB, Miller W. Scoring pairwise genomic sequence alignments. Pac Symp Biocomput. 2002:115-26. PMID: 11928468

Kent WJ, Baertsch R, Hinrichs A, Miller W, Haussler D. Evolution's cauldron: duplication, deletion, and rearrangement in the mouse and human genomes. Proc Natl Acad Sci U S A. 2003 Sep 30;100(20):11484-9. PMID: 14500911; PMC: PMC208784

Schwartz S, Kent WJ, Smit A, Zhang Z, Baertsch R, Hardison RC, Haussler D, Miller W. Human-mouse alignments with BLASTZ. Genome Res. 2003 Jan;13(1):103-7. PMID: 12529312; PMC: PMC430961