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PDE4D — PRKAR2A
Text-mined interactions from Literome
Beard et al., J Biol Chem 2000
:
Mutation of the PKA substrate site in UCR1 ( Ser-54 ) to aspartic acid, which mimics the
activation of
PDE4D3 by
PKA , profoundly reduced the interaction between UCR1 and UCR2
Dodge et al., EMBO J 2001
:
Disruption of
PKA- mAKAP interaction
prevents this enhancement of
PDE4D3 activity, suggesting that the proximity of both enzymes in the mAKAP signaling complex forms a negative feedback loop to restore basal cAMP levels
Ang et al., J Neurochem 2002
(Second Messenger Systems) :
Together, these data show reciprocal
regulation of PDE1C and
PDE4D by
PKA , which represents a novel scheme for plasticity in intracellular signalling
Carlisle Michel et al., Biochem J 2004
:
In vitro and cellular experiments demonstrate that
PKA-phosphorylation of PDE4D3 on Ser-13
increases the affinity of
PDE4D3 for mAKAP
Dodge-Kafka et al., Nature 2005
(Hypertrophy) :
Anchored
PKA stimulates
PDE4D3 to reduce local cAMP concentrations, whereas an mAKAP associated ERK5 kinase module suppresses PDE4D3
Millen et al., Eur J Cell Biol 2006
(Anoxia...) :
Despite the hypoxia induced increases in the expression of PDE4A10/11, PDE4B2 and
PDE4D5 and
activation of certain of these long PDE4 isoforms through
PKA phosphorylation, we suggest that the failure to see any overall increase in PDE4 activity is due to ERK mediated phosphorylation and inhibition of particular PDE4 long isoforms
Bolger et al., Biochem J 2006
:
siRNA ( small interfering RNA ) -mediated knockdown of RACK1 in HEK-293 ( human embryonic kidney ) B2 cells increased beta-arrestin scaffolded PDE4D5 approx. 5-fold, increased
PDE4D5 recruited to the beta2AR ( beta2-adrenergic receptor ) upon isoproterenol challenge approx. 4-fold and severely
attenuated ( approx. 4-5 fold ) both isoproterenol stimulated
PKA ( protein kinase A ) phosphorylation of the beta2AR and activation of ERK ( extracellular-signal regulated kinase )
Levallet et al., Biol Reprod 2007
:
Resensitization of the cAMP response to FSH in 20-day-old Sertoli cells was also associated with the highest FSH induced transient increase in both soluble and particulate PDE4 activities, which suggests developmental changes in the
PKA mediated upregulation of the catalytic activities of long
PDE4D
Murthy et al., Am J Physiol Gastrointest Liver Physiol 2008
:
We have previously shown that cAMP levels are regulated by
PKA mediated phosphorylation of cAMP-specific phosphodiesterase 3A (PDE3A) and
PDE4D5 ; the latter is the only PDE4D isoform expressed in smooth muscle
Bruss et al., J Biol Chem 2008
:
This effect of PDE4D ablation was in large part due to inactivation of a negative feedback mechanism consisting of the
PKA mediated
activation of
PDE4D in response to elevated cAMP levels, as indicated by experiments using the cAMP dependent protein kinase inhibitors H89 and PKI
Dodge-Kafka et al., J Biol Chem 2010
:
Accordingly, expression of a B56delta mutant that can not be phosphorylated by
PKA results in increased
PDE4D3 phosphorylation
Oliveira et al., PloS one 2010
:
Simulations further demonstrate that generation of the cAMP microdomain requires a pool of PDE4D anchored in the cytosol and also requires
PKA mediated phosphorylation of
PDE4D which increases its activity
Sakakibara et al., Neuroendocrinology 1998
:
We hypothesize that
activation of
PDE4D3 by
PKA may constitute a negative feedback signaling pathway which participates in the regulation of cAMP levels