◀ Back to STAT3
IL12A — STAT3
Pathways - manually collected, often from reviews:
-
NCI Pathway Database IL12-mediated signaling events:
STAT3 (STAT3)
→
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
(modification, collaborate)
Jacobson et al., J Exp Med 1995, Zou et al., J Biol Chem 1997
Evidence: assay, other species
-
NCI Pathway Database IL12-mediated signaling events:
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
→
STAT3 (dimer)-active complex (STAT3)
(modification, activates)
Jacobson et al., J Exp Med 1995, Zou et al., J Biol Chem 1997
Evidence: assay, other species
-
NCI Pathway Database IL12-mediated signaling events:
STAT3 (STAT3)
→
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
(modification, collaborate)
Kusaba et al., J Biol Chem 2005
Evidence: mutant phenotype, assay, physical interaction
-
NCI Pathway Database IL27-mediated signaling events:
STAT1-3 (dimer) (STAT1/STAT3)
→
IL12A (IL12A)
(transcription, activates)
Pflanz et al., J Immunol 2004
-
NCI Pathway Database IL12-mediated signaling events:
STAT3 (STAT3)
→
IL12/IL12R/TYK2/JAK2 complex (IL12A-IL12B-IL12RB1-IL12RB2-TYK2-JAK2)
(modification, collaborate)
Gollob et al., J Immunol 1999
Evidence: mutant phenotype, assay
Text-mined interactions from Literome
Gollob et al., J Immunol 1999
:
A specific inhibitor of p38 MAP kinase completely inhibits the serine phosphorylation of STAT1 and
STAT3 induced by
IL-12 and IL-2 and abrogates the functional synergy between IL-12 and IL-2 without affecting STAT tyrosine phosphorylation
Bright et al., J Immunol 1999
(Encephalomyelitis, Autoimmune, Experimental) :
Inhibition of either Jak-2 or Tyk-2 leads to a decrease in the
IL-12 induced tyrosine phosphorylation of
Stat3 , but not of Stat4, protein
Hoentjen et al., Blood 2005
:
Chromatin immunoprecipitation demonstrated enhanced NF-kappaB ( cRel, RelA ) binding to the
IL-12p40 promoter in IL-10 ( -/- ) but not WT BMDCs. Interestingly, LPS
induced STAT3 phosphorylation in WT but not IL-10 ( -/- ) BMDCs, a process blocked by IL-10 receptor blocking antibody
Muthian et al., J Clin Immunol 2004
(Disease Models, Animal...) :
In vitro treatment of activated T cells with quercetin blocks
IL-12 induced tyrosine phosphorylation of JAK2, TYK2,
STAT3 , and STAT4, resulting in a decrease in IL-12 induced T cell proliferation and Th1 differentiation
Shirota et al., J Immunol 2004
(Autoimmune Diseases) :
Findings indicate that these ODN inhibit IFN-gamma induced STAT1 phosphorylation and
IL-12 induced
STAT3 and STAT4 phosphorylation
Kusaba et al., J Biol Chem 2005
:
IL-12 induced phosphorylation of Stat3 on Ser-727 was affected by rapamycin, which may be due to the effect of rapamycin on the
IL-12 induced interaction between mammalian target of rapamycin (mTOR) and
Stat3
Molenda et al., Mol Immunol 2006
:
In keratinocytes,
IL-12 activates
STAT3 and STAT4 ; surprisingly, despite activating these transcription factors, the transcriptional effects of IL-12 did not rise above background levels
Muthian et al., J Neurosci Res 2006
(Disease Models, Animal...) :
Treatment of activated T cells with 1,25 ( OH ) 2D3 also inhibited the
IL-12 induced tyrosine phosphorylation of JAK2, TYK2,
STAT3 , and STAT4 in association with a decrease in T cell proliferation in vitro
Li et al., J Immunol 2006
(Encephalomyelitis, Autoimmune, Experimental) :
Indeed, in the present study we found that bone marrow derived DCs transduced with SOCS-3 significantly inhibited
IL-12 induced
activation of Stat4 and IL-23 induced activation of
Stat3
Stewart et al., Cancer Cell 2009
(Neoplasms) :
In this issue of Cancer Cell, Kortylewski et al. show that in the tumor microenvironment,
STAT3 enhances the expression of the protumor cytokine IL-23 in macrophages but
inhibits the antitumor cytokine
IL-12 in dendritic cells
Kortylewski et al., Cancer Cell 2009
(Neoplasms) :
Furthermore,
Stat3 inhibits NF-kappaB/c-Rel dependent
IL-12/p35 gene expression in tumor associated dendritic cells
Shanmugarajan et al., J Cell Biochem 2009
:
Chromatin immunoprecipitation ( ChIP ) assay confirmed
STAT-3 , but not STAT-1 binding to the OIP-1 gene promoter in
response to
IL-12 stimulation
Jacobson et al., J Exp Med 1995
:
IL-12 selectively
induced nuclear DNA binding complexes that contained
Stat3 and Stat4, recently cloned members of the family of signal transducers and activators of transcription ( STATs )
Yu et al., J Immunol 1996
:
In contrast to IL-2 and IFN-alpha,
IL-12 induced strong tyrosine phosphorylation of STAT4 and variable weak phosphorylation of
STAT3 ... However, supershift analyses using the c-fos promoter sis-inducible element probe showed that
IL-12 activated STAT4, STAT1 alpha, and
STAT3 , and induced complexes containing STAT4 only, STAT4 with STAT1 alpha, STAT3 with STAT1 alpha, or STAT1 alpha only in preactivated primary NK cells