J Biol Chem 1997,
PMID: 9162082
Reif, K; Burgering, B M; Cantrell, D A
Phosphatidylinositol 3-kinase (PI 3-kinase) is activated by the cytokine interleukin-2 (IL-2). We have used a constitutively active PI 3-kinase to identify IL-2-mediated signal transduction pathways directly regulated by PI 3-kinase in lymphoid cells. The serine/threonine protein kinase B (PKB)/Akt can act as a powerful oncogene in T cells, but its positioning in normal T cell responses has not been explored. Herein, we demonstrate that PKB is activated by IL-2 in a PI 3-kinase-dependent fashion. Importantly, PI 3-kinase signals are sufficient for PKB activation in IL-2-dependent T cells, and PKB is a target for PI 3-kinase signals in IL-2 activation pathways. The present study establishes also that PI 3-kinase signals or PKB signals are sufficient for activation of p70 S6 kinase in T cells. PI 3-kinase can contribute to, but is not sufficient for, activation of extracellular signal-regulated kinases (Erks) and Erk effector pathways. Therefore, PI 3-kinase is a selective regulator of serine/threonine kinase signal transduction pathways in T lymphocytes, and this enzyme provides a crucial link between the interleukin-2 receptor, the protooncogene PKB, and p70 S6 kinase.
Document information provided by NCBI PubMed
Text Mining Data
PI 3-kinase → interleukin-2 (IL-2): "
Phosphatidylinositol 3-kinase (
PI 3-kinase ) is
activated by the cytokine
interleukin-2 (IL-2)
"
Phosphatidylinositol 3-kinase → interleukin-2 (IL-2): "
Phosphatidylinositol 3-kinase ( PI 3-kinase ) is activated by the cytokine interleukin-2 (IL-2)
"
PKB → IL-2: "
Herein, we demonstrate that PKB is activated by IL-2 in a PI 3-kinase dependent fashion
"
Manually curated Databases
-
NCI Pathway Database IL2 signaling events mediated by PI3K:
None
→
AKT1 (AKT1)
(modification, activates)
Evidence: mutant phenotype, assay, other species
-
NCI Pathway Database IL2 signaling events mediated by PI3K:
None
→
IL2/IL2R alpha/beta/gamma/JAK1/LCK/JAK3/SHC/GRB2/SOS1/GAB2/SHP2/PI3K complex (IL2RA-IL2RB-IL2RG-IL2-JAK1-LCK-JAK3-SHC1-GRB2-SOS1-GAB2-PTPN11-PIK3CA-PIK3R1)
(modification, activates)
Evidence: mutant phenotype, assay, other species
-
NCI Pathway Database IL2 signaling events mediated by PI3K:
mTOR (MTOR)
→
p70S6K (RPS6KB1)
(modification, activates)
Evidence: mutant phenotype, other species
-
NCI Pathway Database IL2 signaling events mediated by PI3K:
mTOR (MTOR)
→
None
(modification, activates)
Evidence: mutant phenotype, other species
In total, 92 gene pairs are associated to this article in curated databases