Gene interactions and pathways from curated databases and text-mining
Science signaling 2010, PMID: 20371770

AAA+ proteins RUVBL1 and RUVBL2 coordinate PIKK activity and function in nonsense-mediated mRNA decay.

Izumi, Natsuko; Yamashita, Akio; Iwamatsu, Akihiro; Kurata, Rie; Nakamura, Hiroki; Saari, Bonnie; Hirano, Hisashi; Anderson, Philip; Ohno, Shigeo

Phosphatidylinositol 3-kinase-related protein kinase (PIKK) family proteins play essential roles in DNA-based and RNA-based processes, such as the response to DNA damage, messenger RNA (mRNA) quality control, transcription, and translation, where they contribute to the maintenance of genome integrity and accurate gene expression. The adenosine triphosphatases associated with diverse cellular activities (AAA+) family proteins RuvB-like 1 (RUVBL1) and RUVBL2 are involved in various cellular processes, including transcription, RNA modification, DNA repair, and telomere maintenance. We show that RUVBL1 and RUVBL2 associate with each PIKK family member. We also show that RUVBL1 and RUVBL2 control PIKK abundance at least at the mRNA level. Knockdown of RUVBL1 or RUVBL2 decreased PIKK abundance and impaired PIKK-mediated signaling. Analysis of SMG-1, a PIKK family member involved in nonsense-mediated mRNA decay (NMD), revealed an essential role for RUVBL1 and RUVBL2 in NMD. RUVBL1 and RUVBL2 associated with SMG-1 and the messenger ribonucleoproteins in the cytoplasm and promoted the formation of mRNA surveillance complexes during NMD. Thus, RUVBL1 and RUVBL2 regulate PIKK functions on two different levels: They control the abundance of PIKKs, and they stimulate the formation of PIKK-containing molecular complexes, such as those involved in NMD.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: RBM8A — UPF3B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — PIH1D1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — TELO2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — TTI1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF1 — UPF3B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — PIH1D1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF4A3 — UPF1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF1 — SMG7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — TRRAP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — TRRAP (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL2 — POLR2E (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMG8 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMG9 — RBM8A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF2 — RBM8A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — UPF1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — SMG8 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — RPAP3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — POLR2E (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMG9 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — SMG7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — PABPC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAGOH — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF4A3 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — SMG9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — PABPC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBG1 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF1 — RUVBL2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — URI1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — RPAP3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: POLR2E — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF2 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — TUBB (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — POLR2E (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — RBM8A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — SMG5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — TUBA1A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMG1 — HSP90AA1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL2 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — PRKDC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — HSPA4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1A — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — UPF1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — NCBP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — RBM8A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — RBM8A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — URI1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — SMG8 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — RUVBL2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TUBA1A — TTI1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF1 — SMG5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HSPA4 — TTI1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — PRKDC (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF2 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — RUVBL2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM8A — EIF4A3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — RUVBL1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF2 — UPF1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: UPF1 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — RUVBL2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TTI1 — SMG1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — TELO2 (physical association, affinity chromatography technology)
  • IRef Intact Interaction: Complex of SMG8-TTI1-POLR2E-SMG9-SMG1-RUVBL2-RUVBL1 (association, pull down)
  • IRef Intact Interaction: Complex of 11 proteins (association, pull down)
  • IRef Intact Interaction: Complex of POLR2E-RUVBL1-TTI1 (association, pull down)
  • IRef Intact Interaction: Complex of 14 proteins (association, pull down)
  • IRef Intact Interaction: Complex of RUVBL2-TTI1-RUVBL1 (association, pull down)
  • IRef Intact Interaction: Complex of SMG1-RUVBL1-TTI1-RUVBL2 (association, pull down)
  • IRef Intact Interaction: Complex of 21 proteins (association, pull down)
  • IRef Intact Interaction: Complex of RUVBL1-TTI1-POLR2E-RUVBL2-SMG1 (association, anti bait coimmunoprecipitation)
  • IRef Intact Interaction: Complex of RUVBL2-POLR2E-TTI1 (association, anti bait coimmunoprecipitation)
In total, 163 gene pairs are associated to this article in curated databases