Gene interactions and pathways from curated databases and text-mining
J Biol Chem 2005, PMID: 16183647

Redox regulation of the nutrient-sensitive raptor-mTOR pathway and complex.

Sarbassov, Dos D; Sabatini, David M

The raptor-mTOR protein complex is a key component of a nutrient-sensitive signaling pathway that regulates cell size by controlling the accumulation of cellular mass. How nutrients regulate signaling through the raptor-mTOR complex is not well known. Here we show that a redox-sensitive mechanism regulates the phosphorylation of the raptor-mTOR effector S6K1, the interaction between raptor and mTOR, and the kinase activity of the raptor-mTOR complex. In cells treated with the oxidizing agents diamide or phenylarsine oxide, S6K1 phosphorylation increased and became insensitive to nutrient deprivation. Conversely, the reducing reagent BAL (British anti-Lewisite, also known as 2,3-dimercapto-1-propanol) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient-deprived conditions. Our findings suggest that a redox-based signaling mechanism may participate in regulating the nutrient-sensitive raptor-mTOR complex and pathway.

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Text Mining Data

S6K1 ⊣ BAL: " Conversely, the reducing reagent BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol ) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions "

raptor → BAL: " Conversely, the reducing reagent BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol ) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions "

mTOR → BAL: " Conversely, the reducing reagent BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol ) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions "

Manually curated Databases

  • IRef Biogrid Interaction: RICTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MLST8 — MTOR (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS6KB1 — MTOR (direct interaction, enzymatic study)
  • IRef Biogrid Interaction: RPTOR — MTOR (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: RPTOR — RPS6KB1 (in vitro)
In total, 5 gene pairs are associated to this article in curated databases