J Biol Chem 2005,
PMID: 16183647
Sarbassov, Dos D; Sabatini, David M
The raptor-mTOR protein complex is a key component of a nutrient-sensitive signaling pathway that regulates cell size by controlling the accumulation of cellular mass. How nutrients regulate signaling through the raptor-mTOR complex is not well known. Here we show that a redox-sensitive mechanism regulates the phosphorylation of the raptor-mTOR effector S6K1, the interaction between raptor and mTOR, and the kinase activity of the raptor-mTOR complex. In cells treated with the oxidizing agents diamide or phenylarsine oxide, S6K1 phosphorylation increased and became insensitive to nutrient deprivation. Conversely, the reducing reagent BAL (British anti-Lewisite, also known as 2,3-dimercapto-1-propanol) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient-deprived conditions. Our findings suggest that a redox-based signaling mechanism may participate in regulating the nutrient-sensitive raptor-mTOR complex and pathway.
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Text Mining Data
S6K1 ⊣ BAL: "
Conversely, the reducing reagent
BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol )
inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions
"
raptor → BAL: "
Conversely, the reducing reagent BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol ) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions
"
mTOR → BAL: "
Conversely, the reducing reagent BAL ( British anti-Lewisite, also known as 2,3-dimercapto-1-propanol ) inhibits S6K1 phosphorylation and stabilizes the interaction of mTOR and raptor to mimic the state of the complex under nutrient deprived conditions
"
Manually curated Databases
-
IRef Biogrid Interaction:
RICTOR
—
MTOR
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
MLST8
—
MTOR
(physical association, affinity chromatography technology)
-
IRef Biogrid Interaction:
RPS6KB1
—
MTOR
(direct interaction, enzymatic study)
-
IRef Biogrid Interaction:
RPTOR
—
MTOR
(physical association, affinity chromatography technology)
-
IRef Hprd Interaction:
RPTOR
—
RPS6KB1
(in vitro)
In total, 5 gene pairs are associated to this article in curated databases